Zhongshan Daxue xuebao. Yixue kexue ban, 2004 ［目的 l 构建人肝豆状核变性病ATP7B基因野生型及常见突变型的小鼠肝脏特异性表达载体 ALB-ATP7B, 为制备转基因小鼠及相关基因治疗作准备。【方法】定点突变法获取人群中常见的Arg778Leu及 Hisl069Gln两种ATP7B基因突变体，定向克隆将小鼠白蛋白启动子ALB序列及野生和突变的ATP7B基因亚克 隆至真核表达载体kbpala上， 得到可在小鼠肝脏特异性表达的人ATP7B基因正常及突变型真核表达载体 ALB-ATP7B。【结果】经测序及酶切鉴定证实， 真核表达载体ALB ...
doaj
[Transarterial embolization microspheres for liver cancer: current applications and advances]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoLi L, Zhang Y, Zhan Q, Zhao W, Fan J.
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Jianzhu Gangjiegou Jinzhan装配式轻钢复合墙体是一种集承重保温围护一体化的新型墙体。为研究轻钢复合墙体的抗震性能，以轻钢龙骨形式和填充材料类型为变化参数，设计了4个足尺试件进行低周往复荷载试验，得到了各试件的水平承载力、滞回特性和破坏特征，并分析了结构的抗侧刚度、耗能能力和水平承载力。结果表明：墙体轻钢骨架与内填材料协同工作性能良好，墙体的最终破坏表现为钢骨架强度破坏。水平龙骨由C型钢调整为双面扁钢可提高其水平承载力，但会降低墙体的延性和耗能能力；相较于内填泡沫混凝土填充墙体，内填聚苯颗粒泡沫混凝土墙体的水平承载力 ...
王彤彤, 周学军, 王卫东, 王振
doaj
[The mitochondrial protein encoded by circUSP25 promotes colon cancer cell proliferation and migration]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoLi X +6 more
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Jianzhu Gangjiegou Jinzhan平面移动式立体车库容量大、形式多样，对车位分配的要求更高，载荷形式更多样。因此在研究立体车库的载荷谱时，以平面移动式立体车库为代表对其进行研究，运用概率统计方法，基于车辆存取策略建立了适用于立体停车结构的交变载荷谱；进一步采用四点雨流计数法对载荷谱进行统计和边缘分布拟合，结果表明：载荷均值服从均值为9.876，标准差为0.493的正态分布；幅值服从形状参数为1.603，尺度参数为2.048的双参数winbull分布。通过参数外推得到整个生命周期的荷载频次，建立了对应的8级二维载荷谱和一维载荷谱 ...
陈怡芝, 贺拥军
doaj
[CD19-targeted in vivo CAR-T cell therapy for high-risk/refractory B-acute lymphoblastic leukemia: a case report and literature review]. [PDF]
Zhonghua Xue Ye Xue Za ZhiLi Y, Ma FY, Zhang LH, Ren J, Wang SB.
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[A simulation study on the differences in compressive mechanical properties of Voronoi porous structures with two strut-diameter morphologies]. [PDF]
Sheng Wu Yi Xue Gong Cheng Xue Za ZhiMen Y, Wang T, Wu M, Zhang C.
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[Research advances in nanobiotechnology targeting reactive oxygen species for radiation injury repair]. [PDF]
Sheng Wu Yi Xue Gong Cheng Xue Za ZhiGuo C +5 more
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[Research progress on the application of pollen in the biomedical field]. [PDF]
Sheng Wu Yi Xue Gong Cheng Xue Za ZhiBai L, Wang Y, Yang L, Wang Y, Sun Y.
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[Research progress on the application of stem cells and their derivatives in chronic wound repair]. [PDF]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za ZhiWu RX +8 more
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