Results 11 to 20 of about 685,892 (317)

Computer modeling 16S ribosomal RNA [PDF]

Journal of Molecular Biology, 1990
A three-dimensional structure for 16 S RNA has been produced with a computer protocol that is not dependent on human intervention. This protocol improves upon traditional modeling techniques by using distance geometry to fold the molecule in an objective and reproducible fashion.
John E. Hearst, John M. Hubbard
core   +8 more sources

Probing complex RNA structures by mechanical force [PDF]

European Physical Journal E 12 (2003) 605-615, 2003
RNA secondary structures of increasing complexity are probed combining single molecule stretching experiments and stochastic unfolding/refolding simulations. We find that force-induced unfolding pathways cannot usually be interpretated by solely invoking successive openings of native helices.
A. Xayaphoummine, Allemand, Cluzel, Cocco, D. Chatenay, Essevaz-Roulet, Evers, Fersht, Fontana, Gerland, Gutell, H. Isambert, Isambert, J-F. Léger, J. Robert, Kellermayer, Liphardt, Lubensky, Léger, Mathews, Montanari, Nowotny, Onoa, Powers, Rief, Rief, Rief, S. Harlepp, Smith, Svoboda, T. Marchal, Treiber, Treiber, Wimberly, Woodson, Xayaphoummine, Yusupov   +36 more
arxiv   +5 more sources

Selection of reference genes for expression analysis using quantitative real-time PCR in the pea aphid, Acyrthosiphon pisum (Harris) (Hemiptera, Aphidiae). [PDF]

PLoS ONE, 2014
To facilitate gene expression study and obtain accurate qRT-PCR analysis, normalization relative to stable expressed housekeeping genes is required. In this study, expression profiles of 11 candidate reference genes, including actin (Actin), elongation ...
Chunxiao Yang, Huipeng Pan, Yong Liu, Xuguo Zhou   +3 more
doaj   +5 more sources

16S-ITGDB: An Integrated Database for Improving Species Classification of Prokaryotic 16S Ribosomal RNA Sequences [PDF]

Frontiers in Bioinformatics, 2022
Analyzing 16S ribosomal RNA (rRNA) sequences allows researchers to elucidate the prokaryotic composition of an environment. In recent years, third-generation sequencing technology has provided opportunities for researchers to perform full-length sequence
Yu-Peng Hsieh, Yuan-Mao Hung, Mong-Hsun Tsai, Mong-Hsun Tsai, Liang-Chuan Lai, Liang-Chuan Lai, Eric Y. Chuang, Eric Y. Chuang, Eric Y. Chuang, Eric Y. Chuang   +9 more
doaj   +2 more sources

Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling [PDF]

Frontiers in Cellular and Infection Microbiology, 2022
Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies.
Vitor Heidrich, Vitor Heidrich, Lilian T. Inoue, Paula F. Asprino, Fabiana Bettoni, Antonio C. H. Mariotti, Diogo A. Bastos, Denis L. F. Jardim, Marco A. Arap, Anamaria A. Camargo   +9 more
doaj   +2 more sources

Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA. [PDF]

PLoS ONE, 2015
Pathogenic Leptospira species cause a prevalent yet neglected zoonotic disease with mild to life-threatening complications in a variety of susceptible animals and humans. Diagnosis of leptospirosis, which primarily relies on antiquated serotyping methods,
Brian T Backstedt, Ozlem Buyuktanir, Janet Lindow, Elsio A Wunder, Mitermayer G Reis, Sahar Usmani-Brown, Michel Ledizet, Albert Ko, Utpal Pal   +8 more
doaj   +6 more sources

16S ribosomal RNA gene-based metagenomics: A review

Biomedical Research Journal, 2020
With the advent of contemporary molecular tools, the conventional microbiological isolation, enrichment techniques, and approaches have changed considerably.
Asmita Kamble, Shriya Sawant, Harinder Singh   +2 more
doaj   +3 more sources

Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca [PDF]

Scientific Reports, 2021
We evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation.
Nadia Darwish, Jonathan Shao, Lori L. Schreier, Monika Proszkowiec-Weglarz   +3 more
doaj   +2 more sources

Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene [PDF]

BMC Bioinformatics, 2018
Background Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between ...
Francesco Sambo, Francesca Finotello, Enrico Lavezzo, Giacomo Baruzzo, Giulia Masi, Elektra Peta, Marco Falda, Stefano Toppo, Luisa Barzon, Barbara Di Camillo   +9 more
doaj   +2 more sources

Novel and Efficient Method for Diagnosing Infective Endocarditis Using 16S Ribosomal RNA Gene Amplicon Sequence [PDF]

JACC: Case Reports, 2023
We present 2 cases that both developed infective endocarditis and underwent mitral valve replacement. In addition to positive blood culture and echocardiographic findings, such as vegetation or mitral valve perforation, the 16S ribosomal RNA gene ...
Akihisa Kataoka, MD, PhD, Takahiko Shiba, DDS, PhD, Sayaka Katagiri, DDS, PhD, Shuhei Koyama, MD, Kento Kito, MD, Tatsuya Ota, MD, Taiga Katayama, MD, PhD, Yujin Ohsugi, DDS, PhD, Takahiko Nagai, DDS, Ryota Kobayashi, DDS, Keiji Komatsu, DDS, PhD, Masahiro Urata, MD, Tomohiro Imazuru, MD, PhD, Takanori Iwata, DDS, PhD, Ken Kozuma, MD, PhD   +14 more
doaj   +2 more sources