16S ribosomal RNA gene-based metagenomics: A review
Biomedical Research Journal, 2020 With the advent of contemporary molecular tools, the conventional microbiological isolation, enrichment techniques, and approaches have changed considerably.
Asmita Kamble +2 more
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The 16S ribosomal RNA mutation database (16SMDB) [PDF]
Nucleic Acids Research, 1996 The 16S ribosomal RNA mutation database (16SMDB) provides a list of mutated positions in 16S ribosomal RNA from Escherichia coli and the identity of each alteration. Information provided for each mutation includes: (i) a brief description of the phenotype(s) associated with each mutation; (ii) whether a mutant phenotype has been detected by in vivo or ...
Kathleen L. Triman
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16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture in the Investigation of Suspected Bacterial Keratitis. [PDF]
Transl Vis Sci Technol, 2020 Purpose The purpose of this study was to compare conventional diagnostic culture (CDC) to 16S ribosomal RNA polymerase chain reaction (PCR) analysis for diagnosing bacterial keratitis. Methods Samples collected from 100 consecutive patients presenting to
Somerville TF +4 more
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Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene
BMC Bioinformatics, 2018 Background Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between ...
Francesco Sambo +9 more
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Classification of methanogenic bacteria by 16S ribosomal RNA characterization [PDF]
Proceedings of the National Academy of Sciences, 1977 The 16S ribosomal RNAs from 10 species of methanogenic bacteria have been characterized in terms of the oligonucleotides produced by T 1 RNase digestion. Comparative analysis of these data reveals the methanogens to constitute a distinct phylogenetic group containing two major divisions. These organisms appear to be
George E. Fox +4 more
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Comparison of 16S ribosomal RNA hypervariable regions in microbiome studies of anorexia nervosa [PDF]
Frontiers in MicrobiologyIntroductionShort read sequencing of the 16S ribosomal RNA (rRNA) gene targeting specific hypervariable regions is widely employed to study the human gut microbiota.
Arunabh Sharma +10 more
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A systematic algorithm using 16S ribosomal RNA for accurate diagnosis of pneumonia pathogens [PDF]
Scientific ReportsOver half of community-acquired pneumonia cases are caused by a few dozen bacterial species, and accurate identification of these pathogens is essential for effective treatment. In this study, we developed a reliable diagnostic method using 16S ribosomal
Ferry Dwi Kurniawan +5 more
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Detection of Pathogenic Bacteria From Septic Patients Using 16S Ribosomal RNA Gene-Targeted Metagenomic Sequencing. [PDF]
Clin Infect Dis, 2021 Fida M +8 more
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Selection of Peptides Targeting Helix 31 of Bacterial 16S Ribosomal RNA by Screening M13 Phage-Display Libraries [PDF]
Molecules, 2011 Ribosomal RNA is the catalytic portion of ribosomes, and undergoes a variety of conformational changes during translation. Structural changes in ribosomal RNA can be facilitated by the presence of modified nucleotides. Helix 31 of bacterial 16S ribosomal
Christine S. Chow +4 more
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Therapeutic intervention based on gene sequencing analysis of microbial 16S ribosomal RNA of the intrauterine microbiome improves pregnancy outcomes in IVF patients: a prospective cohort study. [PDF]
J Assist Reprod Genet, 2023 Iwami N +7 more
europepmc +3 more sources