Results 21 to 30 of about 80,061 (261)

An HflX-type GTPase from Sulfolobus solfataricus binds to the 50S ribosomal subunit in all nucleotide-bound states [PDF]

open access: yes, 2011
HflX GTPases are found in all three domains of life, Bacteria, Archaea, and Eukaryotes. HflX from Escherichia coli has been shown to bind to the 50S ribosomal subunit in a nucleotide-dependent manner and this interaction strongly stimulates its GTPase ...
Fabian Blombach   +27 more
core   +1 more source

A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity [PDF]

open access: yes, 2012
Background: The 16S rRNA gene is the gold standard in molecular surveys of bacterial and archaeal diversity, but it has the disadvantages that it is often multiple-copy, has little resolution below the species level and cannot be readily interpreted in ...
Kowalchuk, GA   +30 more
core   +1 more source

Molecular biological methods for studying the gut microbiota : the EU human gut flora project [PDF]

open access: yes, 2002
Seven European laboratories co-operated in a joint project (FAIR CT97-3035) to develop, refine and apply molecular methods towards facilitating elucidation of the complex composition of the human intestinal microflora and to devise robust methodologies ...
Vos, W.M., de   +5 more
core   +1 more source

Role of 16‐S RNA in Ribosome Messenger Recognition [PDF]

open access: yesEuropean Journal of Biochemistry, 1980
The deoxyoctanucleotide (5′‐3′)d(A‐A‐G‐G‐A‐G‐G‐T), which is complementary to the 3′ end of 16‐S RNA, inhibits the formation of the complex between the 30‐S subunit and MS2 RNA described in the preceding paper. If the complex is preformed, the octanucleotide cannot prevent entry of the complex into the ribosome cycle upon supplementation with the ...
C, Backendorf   +5 more
openaire   +2 more sources

Rna-Protein Interactions in 30s Ribosomal Subunits Folding and Function of 16s Ribosomal Rna [PDF]

open access: yes, 1989
Chemical probing methods have been used to "footprint" 16S ribosomal RNA (rRNA) at each step during the in vitro assembly of twenty 30S subunit ribosomal proteins.
Noller, H. F.   +3 more
core   +1 more source

PARK(ing) time–How park deficiency affects the biological clock in a Drosophila model of Parkinson's disease

open access: yesFEBS Letters, EarlyView.
Drosophila park mutants serve as a model for Parkinson's disease. We used this strain to investigate the connection between oxidative stress and the circadian clock mechanism. We showed that increased oxidative stress affects the physiology of pacemaker cells, disrupting their daily structural plasticity. Lack of rhythmic signaling from pacemaker cells
Kamila Zientara   +3 more
wiley   +1 more source

Bakteri ribosoomide uurimus keemilise modifitseerimise meetoditega [PDF]

open access: yes, 2010
Väitekirja elektrooniline versioon ei sisalda publikatsioone.Ribosoom on suur makromolekulaarne kompleks, mis kodeerib päriliku informatsiooni valgulisse olemusse.
Pulk, Arto
core  

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

open access: yesMolecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons [PDF]

open access: yesJournal of Visualized Experiments, 2014
One of the major questions in microbial ecology is "who is there?" This question can be answered using various tools, but one of the long-lasting gold standards is to sequence 16S ribosomal RNA (rRNA) gene amplicons generated by domain-level PCR reactions amplifying from genomic DNA.
Sanschagrin, Sylvie, Yergeau, Étienne
openaire   +2 more sources

Reconstructing phylogeny from RNA secondary structure via simulated evolution [PDF]

open access: yes, 2002
DNA sequences of genes encoding functional RNA molecules (e.g., ribosomal RNAs) are commonly used in phylogenetics (i.e. to infer evolutionary history).
Fischer, Will, Geard, Nicholas
core  

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