Results 11 to 20 of about 151,383 (289)
16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture in the Investigation of Suspected Bacterial Keratitis [PDF]
Transl Vis Sci Technol, 2020 Purpose The purpose of this study was to compare conventional diagnostic culture (CDC) to 16S ribosomal RNA polymerase chain reaction (PCR) analysis for diagnosing bacterial keratitis. Methods Samples collected from 100 consecutive patients presenting to
Tobi F Somerville +4 more
semanticscholar +2 more sources
Updating the 97% identity threshold for 16S ribosomal RNA OTUs [PDF]
Bioinformatics, 2017 Abstract The 16S ribosomal RNA (rRNA) gene is widely used to survey microbial communities. Sequences are often clustered into Operational Taxonomic Units (OTUs) as proxies for species. The canonical clustering threshold is 97% identity, which was proposed in 1994 when few 16S rRNA sequences were available, motivating a reassessment on ...
Robert C. Edgar
semanticscholar +3 more sources
Comparison of 16S ribosomal RNA hypervariable regions in microbiome studies of anorexia nervosa [PDF]
Frontiers in MicrobiologyIntroductionShort read sequencing of the 16S ribosomal RNA (rRNA) gene targeting specific hypervariable regions is widely employed to study the human gut microbiota.
Arunabh Sharma +10 more
doaj +2 more sources
A functional pseudoknot in 16S ribosomal RNA. [PDF]
The EMBO Journal, 1991 Several lines of evidence indicate that the universally conserved 530 loop of 16S ribosomal RNA plays a crucial role in translation, related to the binding of tRNA to the ribosomal A site. Based upon limited phylogenetic sequence variation, Woese and Gutell (1989) have proposed that residues 524-526 in the 530 hairpin loop are base paired with residues
T. Powers, H. Noller
semanticscholar +3 more sources
A systematic algorithm using 16S ribosomal RNA for accurate diagnosis of pneumonia pathogens [PDF]
Scientific ReportsOver half of community-acquired pneumonia cases are caused by a few dozen bacterial species, and accurate identification of these pathogens is essential for effective treatment. In this study, we developed a reliable diagnostic method using 16S ribosomal
Ferry Dwi Kurniawan +5 more
doaj +2 more sources
Nucleic Acids Research, 2018 Ribosomal RNAs are processed from primary transcripts containing 16S, 23S and 5S rRNAs in most bacteria. Maturation generally occurs in a two-step process, consisting of a first crude separation of the major species by RNase III during transcription ...
Ciarán Condon, Laetitia Gilet
exaly +2 more sources
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons [PDF]
Journal of Visualized Experiments, 2014 One of the major questions in microbial ecology is “who is there?” This question can be answered using various tools, but one of the long-lasting gold standards is to sequence 16S ribosomal RNA (rRNA) gene amplicons generated by domain-level PCR ...
Sylvie Sanschagrin, É. Yergeau
semanticscholar +3 more sources
A detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria
Journal of Microbiological Methods, 2007 David Alland +2 more
exaly +2 more sources
The expanding role of 16s ribosomal RNA PCR in the management of patients with infective endocarditis undergoing cardiac surgery [PDF]
Frontiers in Cardiovascular MedicinePiergiorgio Bruno +2 more
exaly +2 more sources
Frontiers in Microbiology, 2022 Ribosome assembly is a complex fundamental cellular process that involves assembling multiple ribosomal proteins and several ribosomal RNA species in a highly coordinated yet flexible and resilient manner.
Vignesh M. P. Babu +3 more
doaj +1 more source