Results 11 to 20 of about 1,073,094 (328)

Reading canonical and modified nucleobases in 16S ribosomal RNA using nanopore native RNA sequencing.

PLoS ONE, 2019
The ribosome small subunit is expressed in all living cells. It performs numerous essential functions during translation, including formation of the initiation complex and proofreading of base-pairs between mRNA codons and tRNA anticodons.
Andrew M Smith, Miten Jain, Logan Mulroney, Daniel R Garalde, Mark Akeson   +4 more
doaj   +2 more sources

16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture in the Investigation of Suspected Bacterial Keratitis. [PDF]

Transl Vis Sci Technol, 2020
Purpose The purpose of this study was to compare conventional diagnostic culture (CDC) to 16S ribosomal RNA polymerase chain reaction (PCR) analysis for diagnosing bacterial keratitis. Methods Samples collected from 100 consecutive patients presenting to
Somerville TF, Corless CE, Sueke H, Neal T, Kaye SB.   +4 more
europepmc   +2 more sources

Aptazyme-Mediated Regulation of 16S Ribosomal RNA [PDF]

Chemistry & Biology, 2010
Developing artificial genetic switches in order to control gene expression via an external stimulus is an important aim in chemical and synthetic biology. Here, we expand the application range of RNA switches to the regulation of 16S rRNA function in Escherichia coli.
Wieland, Markus, Hartig, Jörg S., Berschneider, Barbara, Erlacher, Matthias D.   +3 more
openaire   +4 more sources

The essential nature of YqfG, a YbeY homologue required for 3′ maturation of Bacillus subtilis 16S ribosomal RNA is suppressed by deletion of RNase R

Nucleic Acids Research, 2018
Ribosomal RNAs are processed from primary transcripts containing 16S, 23S and 5S rRNAs in most bacteria. Maturation generally occurs in a two-step process, consisting of a first crude separation of the major species by RNase III during transcription ...
Ciaran Condon, Laetitia Gilet
exaly   +2 more sources

A functional pseudoknot in 16S ribosomal RNA. [PDF]

The EMBO Journal, 1991
Several lines of evidence indicate that the universally conserved 530 loop of 16S ribosomal RNA plays a crucial role in translation, related to the binding of tRNA to the ribosomal A site. Based upon limited phylogenetic sequence variation, Woese and Gutell (1989) have proposed that residues 524-526 in the 530 hairpin loop are base paired with residues
T. Powers, H. Noller
semanticscholar   +3 more sources

Comparison of 16S ribosomal RNA hypervariable regions in microbiome studies of anorexia nervosa [PDF]

Frontiers in Microbiology
IntroductionShort read sequencing of the 16S ribosomal RNA (rRNA) gene targeting specific hypervariable regions is widely employed to study the human gut microbiota.
Arunabh Sharma, Nadia Andrea Andreani, Nadia Andrea Andreani, Lara Keller, Lara Keller, Beate Herpertz-Dahlmann, Jochen Seitz, Jochen Seitz, John F. Baines, John F. Baines, Astrid Dempfle   +10 more
doaj   +2 more sources

A systematic algorithm using 16S ribosomal RNA for accurate diagnosis of pneumonia pathogens [PDF]

Scientific Reports
Over half of community-acquired pneumonia cases are caused by a few dozen bacterial species, and accurate identification of these pathogens is essential for effective treatment. In this study, we developed a reliable diagnostic method using 16S ribosomal
Ferry Dwi Kurniawan, Dina Alia, Mamoru Shiraishi, Megumi Higo, Yoshiaki Inoue, Koichi Hagiwara   +5 more
doaj   +2 more sources

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons [PDF]

Journal of Visualized Experiments, 2014
One of the major questions in microbial ecology is “who is there?” This question can be answered using various tools, but one of the long-lasting gold standards is to sequence 16S ribosomal RNA (rRNA) gene amplicons generated by domain-level PCR ...
Sylvie Sanschagrin, É. Yergeau
semanticscholar   +3 more sources

A detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria

Journal of Microbiological Methods, 2007
David Alland, Soumitesh Chakravorty, Nancy Connell   +2 more
exaly   +2 more sources

Functional Modification of 16S Ribosomal RNA by Kethoxal [PDF]

Proceedings of the National Academy of Sciences, 1972
Kethoxal reacts with 30S ribosomal subunits to give totally inactive particles, as measured by in vitro protein synthesis. It is postulated that functional modification occurs at the binding site for transfer RNA since ( a ) loss of specific binding of ...
H F, Noller, J B, Chaires
openaire   +2 more sources