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Nuclease S1 mapping of 16S ribosomal RNA in ribosomes

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1990
Escherichia coli 16S rRNA and 16S-like rRNAs from other species have several universally conserved sequences which are believed to be single-stranded in ribosomes. The quantitative disposition of these sequences within ribosomes is not known. Here we describe experiments designed to explore the availability of universal 16S rRNA sequences for ...
M A, Rahman, H W, Schaup
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Precursor 16S RNA in Active 30S Ribosomes

Nature, 1974
BIOGENESIS of bacterial ribosomes proceeds through the formation of precursor particles1–4, consisting of an incomplete set of ribosomal protein5 and of RNA molecules that are longer and less methylated than mature RNA4,6,7. RNA maturation is commonly considered to be a part of the ribosome maturation process.
MANGIAROTTI, Giorgio   +3 more
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Conservation of primary structure in 16S ribosomal RNA

Nature, 1975
THERE is no doubt that the large ribosomal RNAs play specific roles in ribosome function1. Yet the thrust of experimentation during the past 5 years indicates clearly that the biologist tends to view these roles as structural, function being reserved by and large for the ribosomal protein components2,3.
C R, Woese   +7 more
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Nucleotide Sequences of Sections of 16S Ribosomal RNA

Nature, 1970
Three large sections of 16S RNA, obtained by partial enzymatic hydrolysis, and together representing nearly one-fifth of the molecule, have been analysed. Knowledge of their primary structure allows features of the secondary structure to be envisaged.
C, Ehresmann, P, Fellner, J P, Ebel
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Secondary Structure of 16 S Ribosomal RNA

Science, 1981
A secondary structure model for 16 S ribosomal RNA which is based on available chemical, enzymatic, and comparative sequence data shows good agreement between constraints dictated by the model and a wide variety of experimental observations.
H F, Noller, C R, Woese
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16S Mitochondrial Ribosomal RNA Degradation Is Associated with Apoptosis

Free Radical Biology and Medicine, 1997
The use of mitochondrial RNA as an indicator of apoptosis was investigated. Exposure of HA-1 fibroblastic cells to 10 micromol H(2)O(2) per 10(7) cells induced nuclear fragmentation, cell shrinkage, and internucleosomal DNA fragmentation, all characteristics of apoptosis.
D R, Crawford   +5 more
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Chromosomal loci for 16S ribosomal RNA in Escherichia coli

Molecular and General Genetics MGG, 1972
Genetic loci for 16S ribosomal RNA (rRNA) on the Escherichia coli chromosome were determined using the K-sequence, a characteristic oligonucleotide of strain K12, as a genetic marker. Oligonucleotide analyses of 16S rRNA from various recombinants between strain K12 and strain B(H) showed that the loci for 16S rRNA containing the K-sequence were near ...
M, Matsubara, R, Takata, S, Osawa
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Images of 16S ribosomal RNA by scanning tunnelling microscopy

Journal of Microscopy, 1991
SUMMARYWe report the use of scanning tunnelling microscopy (STM) to study surface topographies of complex nucleic acid structures. From low‐resolution STM images of uncoated 16S ribosomal RNA, we demonstrate the possibility of determining several objective parameters (molecular mass and radius of gyration) in order to characterize and identify the ...
P J, Flamion   +5 more
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Complementary binding of oligonucleotides with 16S RNA and ribosomal ribonucleoproteins

Molecular Biology Reports, 1975
The accessibility of single-stranded sequences in 16S RNA in free state and in ribonucleoprotein particles (RNP) to complementary binding with isoplith fractions of oligonucleotides was studied. RNP had different protein composition and corresponded to intermediate stages of E. coli 30S subunit assembly in vitro.
A M, Kopylov   +3 more
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Methylation of 16S RNA during ribosome assembly in vitro

Nature, 1974
Methyl deficient 16S RNA from kasugamycin resistant Escherichia coli has been used to study methylation of RNA during ribosome assembly in vitro. Methylation occurs at an intermediate stage and is inhibited at a late stage of assembly. 30S ribosomal proteins required for methylation, and inhibition, have been identified.
P, Thammana, W A, Held
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