Ion torrent-based transcriptional assessment of a Corynebacterium pseudotuberculosis equi strain reveals denaturing high-performance liquid chromatography a promising rRNA depletion method [PDF]
Corynebacterium pseudotuberculosis equi is a Gram-positive pathogenic bacterium which affects a variety of hosts. Besides the great economic losses it causes to horse-breeders, this organism is also known to be an important infectious agent to cattle and
Azarani+44 more
core +1 more source
Euglena gracilis chloroplast ribosomal RNA transcription units. II. Nucleotide sequence homology between the 16 S–23 S ribosomal RNA spacer and the 16 S ribosomal RNA leader regions. [PDF]
The DNA sequences of two segments of the ribosomal RNA transcription units of Euglena gracilis Pringsheim strain Z chloroplast DNA have been determined. The first is from the 16 S to 23 S rRNA spacer region. The nucleotide sequence determined includes 64 bp from the 3'-end of the 16 S rRNA gene, the adjacent 87-bp spacer containing 68A-T base pairs, a ...
J R Dodd+3 more
openaire +2 more sources
Surfaceome: a new era in the discovery of immune evasion mechanisms of circulating tumor cells
In the era of immunotherapies, many patients either do not respond or eventually develop resistance. We propose to pave the way for proteomic analysis of surface‐expressed proteins called surfaceome, of circulating tumor cells. This approach seeks to identify immune evasion mechanisms and discover potential therapeutic targets. Circulating tumor cells (
Doryan Masmoudi+3 more
wiley +1 more source
INTRODUCTION: Amplification of the 16S ribosomal RNA gene by polymerase chain reaction (PCR) followed by analysis of generated sequences can be an important adjunct to conventional cultures.
B Alraddadi, S Al-Azri, KR Forward
doaj +1 more source
Metaxa : a software tool for automated detection and discrimination among ribosomal small subunit (12S/16S/18S) sequences of archaea, bacteria, eukaryotes, mitochondria, and chloroplasts in metagenomes and environmental sequencing datasets [PDF]
Peer ...
Abarenkov, Kessy+9 more
core +1 more source
Correcting a SHAPE-directed RNA structure by a mutate-map-rescue approach [PDF]
The three-dimensional conformations of non-coding RNAs underpin their biochemical functions but have largely eluded experimental characterization. Here, we report that integrating a classic mutation/rescue strategy with high-throughput chemical mapping enables rapid RNA structure inference with unusually strong validation. We revisit a paradigmatic 16S
arxiv +1 more source
Models of protein production along the cell cycle: an investigation of possible sources of noise [PDF]
In this article, we quantitatively study, through stochastic models, the efects of several intracellular phenomena, such as cell volume growth, cell division, gene replication as well as fuctuations of available RNA polymerases and ribosomes. These phenomena are indeed rarely considered in classic models of protein production and no relative ...
arxiv +1 more source
The authors analyzed the spatial distributions of gene and metabolite profiles in cervical cancer through spatial transcriptomic and spatially resolved metabolomic techniques. Pivotal genes and metabolites within these cases were then identified and validated.
Lixiu Xu+3 more
wiley +1 more source
Association between semen microbiome disorder and sperm DNA damage
DNA fragmentation index (DFI), a new biomarker to diagnose male infertility, is closely associated with poor reproductive outcomes. Previous research reported that seminal microbiome correlated with sperm DNA integrity, suggesting that the microbiome may
Junxian He+7 more
doaj +1 more source
The complete mitochondrial genome of Anas penelope (Anatidae: Anas)
The complete mitochondrial DNA genome of the Eurasian Wigeon, Anas penelope, was mapped by the next-generation sequencing and Mega 7.0. The circular mitogenome (16,596 bp in length) contains 13 protein-coding genes, 2 rRNA genes (12S ribosomal RNA and ...
Xiaoping Sun+5 more
doaj +1 more source