Results 61 to 70 of about 694,582 (359)

Ion torrent-based transcriptional assessment of a Corynebacterium pseudotuberculosis equi strain reveals denaturing high-performance liquid chromatography a promising rRNA depletion method [PDF]

, 2013
Corynebacterium pseudotuberculosis equi is a Gram-positive pathogenic bacterium which affects a variety of hosts. Besides the great economic losses it causes to horse-breeders, this organism is also known to be an important infectious agent to cattle and
Azarani, Azarani, Baird, Barakat, Biberstein, Braverman, Bustin, Chen, Chen, Colbert, Conesa, Efstratiou, Gaspar, Hantke, Jones, Jünemann, Lukashin, Martin, Meldrum, Muto, Nguyen, Nolden, Pacheco, Papp-Wallace, Patel, Paton, Pfaffl, Piddington, Pinho, Pinto, Ramos, Ramos, Richardson, Ruiz, Sambrook, Schulz, Sorek, Spier, Swiercz, Trost, Vliet, Weinberg, Westermann, Wong, Zerbino   +44 more
core   +1 more source

Euglena gracilis chloroplast ribosomal RNA transcription units. II. Nucleotide sequence homology between the 16 S–23 S ribosomal RNA spacer and the 16 S ribosomal RNA leader regions. [PDF]

Journal of Biological Chemistry, 1980
The DNA sequences of two segments of the ribosomal RNA transcription units of Euglena gracilis Pringsheim strain Z chloroplast DNA have been determined. The first is from the 16 S to 23 S rRNA spacer region. The nucleotide sequence determined includes 64 bp from the 3'-end of the 16 S rRNA gene, the adjacent 87-bp spacer containing 68A-T base pairs, a ...
J R Dodd, E M Orozco, Richard B. Hallick, K E Rushlow   +3 more
openaire   +2 more sources

Surfaceome: a new era in the discovery of immune evasion mechanisms of circulating tumor cells

Molecular Oncology, EarlyView.
In the era of immunotherapies, many patients either do not respond or eventually develop resistance. We propose to pave the way for proteomic analysis of surface‐expressed proteins called surfaceome, of circulating tumor cells. This approach seeks to identify immune evasion mechanisms and discover potential therapeutic targets. Circulating tumor cells (
Doryan Masmoudi, Jérome Vialaret, Christophe Hirtz, Catherine Alix‐Panabières   +3 more
wiley   +1 more source

Influence of 16S Ribosomal RNA Gene Polymerase Chain Reaction and Sequencing on Antibiotic Management of Bone and Joint Infections

Canadian Journal of Infectious Diseases and Medical Microbiology, 2013
INTRODUCTION: Amplification of the 16S ribosomal RNA gene by polymerase chain reaction (PCR) followed by analysis of generated sequences can be an important adjunct to conventional cultures.
B Alraddadi, S Al-Azri, KR Forward
doaj   +1 more source

Metaxa : a software tool for automated detection and discrimination among ribosomal small subunit (12S/16S/18S) sequences of archaea, bacteria, eukaryotes, mitochondria, and chloroplasts in metagenomes and environmental sequencing datasets [PDF]

, 2011
Peer ...
Abarenkov, Kessy, Bengtsson, Johan, Eriksson, K. Martin, Grelet, Gwen-Aelle, Hartmann, Martin, Nilsson, R. Henrik, Petri, Anna, Rosenblad, Magnus Alm, Shenoy, Belle Damodara, Wang, Zheng   +9 more
core   +1 more source

Correcting a SHAPE-directed RNA structure by a mutate-map-rescue approach [PDF]

, 2014
The three-dimensional conformations of non-coding RNAs underpin their biochemical functions but have largely eluded experimental characterization. Here, we report that integrating a classic mutation/rescue strategy with high-throughput chemical mapping enables rapid RNA structure inference with unusually strong validation. We revisit a paradigmatic 16S
arxiv   +1 more source

Models of protein production along the cell cycle: an investigation of possible sources of noise [PDF]

PLOS ONE 15, no. 1 (January 16, 2020): e0226016, 2017
In this article, we quantitatively study, through stochastic models, the efects of several intracellular phenomena, such as cell volume growth, cell division, gene replication as well as fuctuations of available RNA polymerases and ribosomes. These phenomena are indeed rarely considered in classic models of protein production and no relative ...
arxiv   +1 more source

Combined spatially resolved metabolomics and spatial transcriptomics reveal the mechanism of RACK1‐mediated fatty acid synthesis

Molecular Oncology, EarlyView.
The authors analyzed the spatial distributions of gene and metabolite profiles in cervical cancer through spatial transcriptomic and spatially resolved metabolomic techniques. Pivotal genes and metabolites within these cases were then identified and validated.
Lixiu Xu, Jinqiu Li, Junqi Ma, Ayshamgul Hasim   +3 more
wiley   +1 more source

Association between semen microbiome disorder and sperm DNA damage

Microbiology Spectrum
DNA fragmentation index (DFI), a new biomarker to diagnose male infertility, is closely associated with poor reproductive outcomes. Previous research reported that seminal microbiome correlated with sperm DNA integrity, suggesting that the microbiome may
Junxian He, Menghui Ma, Zhenhan Xu, Jintao Guo, Haicheng Chen, Xing Yang, Peigen Chen, Guihua Liu   +7 more
doaj   +1 more source

The complete mitochondrial genome of Anas penelope (Anatidae: Anas)

Mitochondrial DNA. Part B. Resources, 2020
The complete mitochondrial DNA genome of the Eurasian Wigeon, Anas penelope, was mapped by the next-generation sequencing and Mega 7.0. The circular mitogenome (16,596 bp in length) contains 13 protein-coding genes, 2 rRNA genes (12S ribosomal RNA and ...
Xiaoping Sun, Chaochao Hu, Shuang Li, Mengfan Zhai, Wei Liu, Yinlong Zhang   +5 more
doaj   +1 more source