Results 81 to 90 of about 1,073,094 (328)

Association between semen microbiome disorder and sperm DNA damage

open access: yesMicrobiology Spectrum
DNA fragmentation index (DFI), a new biomarker to diagnose male infertility, is closely associated with poor reproductive outcomes. Previous research reported that seminal microbiome correlated with sperm DNA integrity, suggesting that the microbiome may
Junxian He   +7 more
doaj   +1 more source

Influence of 16S Ribosomal RNA Gene Polymerase Chain Reaction and Sequencing on Antibiotic Management of Bone and Joint Infections

open access: yesCanadian Journal of Infectious Diseases and Medical Microbiology, 2013
INTRODUCTION: Amplification of the 16S ribosomal RNA gene by polymerase chain reaction (PCR) followed by analysis of generated sequences can be an important adjunct to conventional cultures.
B Alraddadi, S Al-Azri, KR Forward
doaj   +1 more source

In Silico and Experimental Evaluation of Primer Sets for Species-Level Resolution of the Vaginal Microbiota Using 16S Ribosomal RNA Gene Sequencing

open access: yesJournal of Infectious Diseases, 2018
Background Identification of bacteria in human vaginal specimens is commonly performed using 16S ribosomal RNA (rRNA) gene sequences. However, studies utilize different 16S primer sets, sequence databases, and parameters for sample and database ...
William J. van der Pol   +7 more
semanticscholar   +1 more source

Roles of the HEAT repeat proteins Utp10 and Utp20 in 40S ribosome maturation [PDF]

open access: yes, 2007
A family of HEAT-repeat containing ribosome synthesis factors was previously identified in Saccharomyces cerevisiae. We report the detailed characterization of two of these factors, Utp10 and Utp20, which were initially identified as components of the ...
Tollervey, David   +2 more
core   +1 more source

p190A/ARHGAP35 and p190B/ARHGAP5 proteins in endometrial cancer: a novel cancer‐relevant paralog interplay

open access: yesMolecular Oncology, EarlyView.
This study identifies ARHGAP5, in addition to the frequently mutated ARHGAP35, as significantly mutated in endometrial cancer. Mutations in both genes co‐occur and are associated with their correlated downregulation. Functional CRISPR studies show that both paralogs regulate similar pathways, including actin cytoskeleton organization.
Mathilde Pinault   +12 more
wiley   +1 more source

Reconstructing phylogeny from RNA secondary structure via simulated evolution

open access: yes, 2002
DNA sequences of genes encoding functional RNA molecules (e.g., ribosomal RNAs) are commonly used in phylogenetics (i.e. to infer evolutionary history).
Fischer, Will, Geard, Nicholas
core   +1 more source

The complete mitochondrial genome of Glossaulax reiniana (Littorinimorpha: Naticidae)

open access: yesMitochondrial DNA. Part B. Resources, 2018
In the present study, the complete mitochondrial genome of Glossaulax reiniana was determined using the next-generation sequencing. The circular genome was found to be 15,254 bp in length and had an overall nucleotide composition of 30.6% A, 14.1% C, 15 ...
Peng-Yu Li   +3 more
doaj   +1 more source

16S Ribosomal RNA-Based Analysis of Thermophilic Bacteria in Gedongsongo Hot Spring

open access: yesMicrobiology Indonesia, 2010
Denaturing gradient gel electrophoresis was used to identify the bacterial community at the Gedongsongo (WGS-2) hot spring. The bacterial samples were obtained from both culture dependent and independent strategies.
AGUSTINA LULUSTYANINGATI NURUL AMININ1   +3 more
doaj   +1 more source

RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components [PDF]

open access: yes, 2004
Sequential events in macromolecular biosynthesis are often elegantly coordinated. The small ribosomal subunit (SSU) processome is a large ribonucleoprotein (RNP) required for processing of precursors to the small subunit RNA, the 18S, of the ribosome. We
Granneman, Sander; id_orcid   +6 more
core   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

open access: yesFEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro   +1 more
wiley   +1 more source

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