Results 1 to 10 of about 403,409 (349)

A comparison of 16S rRNA-gene and 16S rRNA-transcript derived microbial communities in bulk and rhizosphere soils [PDF]

Frontiers in Microbiology
Root exudates in a plant’s rhizosphere alters microbial community membership and activity, which can in turn alter a plant’s health and fitness. In this study we characterized bacterial community composition, using 16S rRNA-gene (DNA) sequencing to ...
Alessandra Ceretto, Alessandra Ceretto, Cynthia Weinig, Cynthia Weinig, Cynthia Weinig   +4 more
doaj   +2 more sources

Variable Region Sequences Influence 16S rRNA Performance

Microbiology Spectrum, 2023
16S rRNA gene sequences are commonly analyzed for taxonomic and phylogenetic studies because they contain variable regions that can help distinguish different genera. However, intra-genus distinction using variable region homology is often impossible due
Nikhil Bose, Sean D. Moore
doaj   +3 more sources

Long-read MinION™ sequencing of 16S and 16S-ITS-23S rRNA genes provides species-level resolution of Lactobacillaceae in mixed communities

Frontiers in Microbiology, 2023
The Lactobacillaceae are lactic acid bacteria harnessed to deliver important outcomes across numerous industries, and their unambiguous, species-level identification from mixed community environments is an important endeavor.
Sandra A. Olivier, Sandra A. Olivier, Michelle K. Bull, Michelle K. Bull, Mikael Lenz Strube, Robert Murphy, Tom Ross, John P. Bowman, Belinda Chapman, Belinda Chapman   +9 more
doaj   +1 more source

Species Identification and Profiling of Complex Microbial Communities Using Shotgun Illumina Sequencing of 16S rRNA Amplicon Sequences [PDF]

, 2013
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Hibberd, Martin Lloyd, Ho, Eliza Xin Pei, Kukkillaya, Vinutha Uppoor, Lay, Christophe, Low, Louie, Nagarajan, Niranjan, Ong, Swee Hoe, Wilm, Andreas   +7 more
core   +13 more sources

Gallstone and bile microbiota: A case-control study based on 16S rRNA gene sequencing. [PDF]

World J Gastrointest Surg
Lu ZX, Jiang YQ, Wang DS, Song YT, Jiang XM, Xu FJ, Tang J, Li B, Huang WH.   +8 more
europepmc   +2 more sources

OTU analysis using metagenomic shotgun sequencing data. [PDF]

PLoS ONE, 2012
Because of technological limitations, the primer and amplification biases in targeted sequencing of 16S rRNA genes have veiled the true microbial diversity underlying environmental samples. However, the protocol of metagenomic shotgun sequencing provides
Xiaolin Hao, Ting Chen
doaj   +1 more source

Latitudinal variation in the potential activity of Atlantic Ocean bacterioplankton revealed through 16S rRNA and 16S rRNA gene metabarcoding

Frontiers in Marine Science, 2023
The activities of bacterioplankton sustain open ocean biogeochemical and ecological processes, however, little is known about the activity of specific bacterioplankton, especially related to their biogeography across oceanic scales.
Ro Allen, Kimberley E. Bird, Kimberley E. Bird, J. Colin Murrell, Michael Cunliffe, Michael Cunliffe   +5 more
doaj   +1 more source

Engineering CRISPR/Cas9 to mitigate abundant host contamination for 16S rRNA gene-based amplicon sequencing

Microbiome, 2020
Background High-throughput sequencing of bacterial 16S rRNA gene (16S-seq) is a useful and common method for studying bacterial community structures. However, contamination of the 16S rRNA genes from the mitochondrion and plastid hinders the sensitive ...
Luyang Song, Kabin Xie
doaj   +1 more source

Strategy for the identification of micro-organisms producing food and feed products : bacteria producing food enzymes as study case [PDF]

, 2020
Recent European regulations require safety assessments of food enzymes (FE) before their commercialization. FE are mainly produced by micro-organisms, whose viable strains nor associated DNA can be present in the final products.
De Keersmaecker, Sigrid C. J., Deckers, Marie, Deforce, Dieter, Denayer, Sarah, Fraiture, Marie-Alice, Heyndrickx, Marc, Roosens, Nancy H. C., Vanneste, Kevin, Winand, Raf   +8 more
core   +2 more sources

Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets

Data in Brief, 2021
The potential for aerobic and anaerobic microbial natural attenuation of PCBs in freshwater sediments is described by PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets generated, in duplicate, from 27 sediment samples ...
Timothy E. Mattes, Jessica M. Ewald, Yi Liang, Andres Martinez, Andrew M. Awad, Keri C. Hornbuckle, Jerald L. Schnoor   +6 more
doaj   +1 more source