Results 1 to 10 of about 417,138 (346)
A comparison of 16S rRNA-gene and 16S rRNA-transcript derived microbial communities in bulk and rhizosphere soils [PDF]
Frontiers in MicrobiologyRoot exudates in a plant’s rhizosphere alters microbial community membership and activity, which can in turn alter a plant’s health and fitness. In this study we characterized bacterial community composition, using 16S rRNA-gene (DNA) sequencing to ...
Alessandra Ceretto +4 more
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Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing
MSphere, 2021 In 16S rRNA gene sequencing, certain bacterial genera were found to be underrepresented or even missing in taxonomic profiles when using unsuitable primer combinations, outdated reference databases, or inadequate pipeline settings.
Monica S Matchado +2 more
exaly +2 more sources
Clinical Microbiology Reviews, 2020 This review provides a state-of-the-art description of the performance of Sanger cycle sequencing of the 16S rRNA gene for routine identification of bacteria in the clinical microbiology laboratory.
Deirdre L Church
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Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations [PDF]
Applied and Environmental Microbiology, 1990 R. Amann +5 more
semanticscholar +2 more sources
PLoS ONE, 2013 16S ribosomal RNA currently represents the most important target of study in bacterial ecology. Its use for the description of bacterial diversity is, however, limited by the presence of variable copy numbers in bacterial genomes and sequence variation ...
Tomáš Větrovský, Petr Baldrian
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Frontiers in Microbiology, 2023 The Lactobacillaceae are lactic acid bacteria harnessed to deliver important outcomes across numerous industries, and their unambiguous, species-level identification from mixed community environments is an important endeavor.
Sandra A. Olivier +9 more
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Variable Region Sequences Influence 16S rRNA Performance
Microbiology Spectrum, 2023 16S rRNA gene sequences are commonly analyzed for taxonomic and phylogenetic studies because they contain variable regions that can help distinguish different genera. However, intra-genus distinction using variable region homology is often impossible due
Nikhil Bose, Sean D. Moore
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OTU analysis using metagenomic shotgun sequencing data. [PDF]
PLoS ONE, 2012 Because of technological limitations, the primer and amplification biases in targeted sequencing of 16S rRNA genes have veiled the true microbial diversity underlying environmental samples. However, the protocol of metagenomic shotgun sequencing provides
Xiaolin Hao, Ting Chen
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Frontiers in Marine Science, 2023 The activities of bacterioplankton sustain open ocean biogeochemical and ecological processes, however, little is known about the activity of specific bacterioplankton, especially related to their biogeography across oceanic scales.
Ro Allen +5 more
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