Results 21 to 30 of about 417,138 (346)
Background High-throughput sequencing of bacterial 16S rRNA gene (16S-seq) is a useful and common method for studying bacterial community structures. However, contamination of the 16S rRNA genes from the mitochondrion and plastid hinders the sensitive ...
Luyang Song, Kabin Xie
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The potential for aerobic and anaerobic microbial natural attenuation of PCBs in freshwater sediments is described by PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets generated, in duplicate, from 27 sediment samples ...
Timothy E. Mattes+6 more
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Combination of 16S rRNA and procalcitonin in diagnosis of neonatal clinically suspected sepsis
Objective To investigate the application of 16S rRNA in diagnosing patients with neonatal sepsis. Methods We studied 60 consecutive neonatal patients with clinically suspected sepsis and 20 non-infective cases as controls.
Renqiang Yu+4 more
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A highly resolved taxonomy for ammonia-oxidizing archaea (AOA) based on the alpha subunit of ammonia monooxygenase (amoA) was recently established, which uncovered novel environmental patterns of AOA, challenging previous generalizations.
Haitao Wang+6 more
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Diagnostic Yield and Impact on Antimicrobial Management of 16S rRNA Testing of Clinical Specimens
16S rRNA gene sequencing is increasingly used in clinical practice for bacterial identification of clinical specimens. However, studies on its applicability to direct clinical specimens are limited. Here, we studied the diagnostic yield and impact of 16S
Pruke Eamsakulrat+2 more
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Reconstructing 16S rRNA genes in metagenomic data [PDF]
Abstract Metagenomic data, which contains sequenced DNA reads of uncultured microbial species from environmental samples, provide a unique opportunity to thoroughly analyze microbial species that have never been identified before. Reconstructing 16S ribosomal RNA, a phylogenetic marker gene, is usually required to analyze the composition
James R. Cole+3 more
openaire +2 more sources
Molecular cloning of the rRNA genes of Prevotella bryantii B14
Parts of the rRNA operons of anaerobic rumen bacteria Prevotella bryantii B14 were cloned. 350 colonies carrying pBluescriptII with HindIII DNA fragments were screened with 16S rDNA specific probe and six positive clones were obtained.
Matjaž PETERKA, Gorazd AVGUŠTIN
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Background: Microscopic, biochemical, molecular, and computer-based approaches are extensively used to identify and classify bacterial populations.
Bobby Paul
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The recent advent of DNA sequencing technologies facilitates the use of genome sequencing data that provide means for more informative and precise classification and identification of members of the Bacteria and Archaea.
Seok-Hwan Yoon+6 more
semanticscholar +1 more source
Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences
Profiling phylogenetic marker genes, such as the 16S rRNA gene, is a key tool for studies of microbial communities but does not provide direct evidence of a community's functional capabilities.
M. Langille+11 more
semanticscholar +1 more source