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Diagnostic Yield and Impact on Antimicrobial Management of 16S rRNA Testing of Clinical Specimens
Microbiology Spectrum, 2022 16S rRNA gene sequencing is increasingly used in clinical practice for bacterial identification of clinical specimens. However, studies on its applicability to direct clinical specimens are limited. Here, we studied the diagnostic yield and impact of 16S
Pruke Eamsakulrat +2 more
doaj +1 more source
Mycoplasma gallisepticum16S rRNA genes [PDF]
FEMS Microbiology Letters, 1995 The genome of Mycoplasma gallisepticum A5969 contains a truncated pseudogene for 16S rRNA in addition to a single unsplit rRNA-operon and a second discontinuous set of rRNA genes. Other M. gallisepticum strains tested do not possess the truncated gene.
A, Skamrov +3 more
openaire +3 more sources
Comparison of five assays for DNA extraction from bacterial cells in human faecal samples [PDF]
, 2020 Aim To determine the most effective DNA extraction method for bacteria in faecal samples. Materials and Results This study assessed five commercial methods, that is, NucliSens easyMag, QIAamp DNA Stool Mini kit, PureLink Microbiome DNA purification kit ...
Glorieux, Griet +3 more
core +1 more source
F1000Research, 2023 Background: Microscopic, biochemical, molecular, and computer-based approaches are extensively used to identify and classify bacterial populations.
Bobby Paul
doaj +1 more source
Taxonomy of anaerobic digestion microbiome reveals biases associated with the applied high throughput sequencing strategies [PDF]
, 2018 In the past few years, many studies investigated the anaerobic digestion microbiome by means of 16S rRNA amplicon sequencing. Results obtained from these studies were compared to each other without taking into consideration the followed procedure for ...
Angelidaki, Irini +4 more
core +3 more sources
High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]
, 2012 The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Cogan, Tristan +4 more
core +3 more sources
mSystems, 2022 Analysis of 16S rRNA (rRNA) genes provides a central means of taxonomic classification of bacterial species. Based on presumed sequence identity among species of the Bacillus cereus sensu lato group, the 16S rRNA genes of B.
Peter Braun +7 more
doaj +1 more source
Reconstructing 16S rRNA genes in metagenomic data [PDF]
Bioinformatics, 2015 Abstract Metagenomic data, which contains sequenced DNA reads of uncultured microbial species from environmental samples, provide a unique opportunity to thoroughly analyze microbial species that have never been identified before. Reconstructing 16S ribosomal RNA, a phylogenetic marker gene, is usually required to analyze the composition
Yuan, Cheng +3 more
openaire +2 more sources
Identifying the Dominant Soil Bacterial Taxa in Libraries of 16S rRNA and 16S rRNA Genes [PDF]
Applied and Environmental Microbiology, 2006 From near to far , from here to there , funny things are everywhere. —Dr. Seuss, One Fish Two Fish Red Fish Blue Fish In 1909, H. Joel Conn ([25][1]) expressed the hope that methods would soon be at hand by which the significance of the different bacteria present in any soil could ...
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Utility of 16S rRNA PCR performed on clinical specimens in patient management
International Journal of Infectious Diseases, 2017 Background: Broad-range 16S rRNA PCR can be used for the detection and identification of bacteria from clinical specimens in patients for whom there is a high suspicion of infection and cultures are negative.
A. Akram +4 more
doaj +1 more source