Results 21 to 30 of about 417,138 (346)

Engineering CRISPR/Cas9 to mitigate abundant host contamination for 16S rRNA gene-based amplicon sequencing

open access: yesMicrobiome, 2020
Background High-throughput sequencing of bacterial 16S rRNA gene (16S-seq) is a useful and common method for studying bacterial community structures. However, contamination of the 16S rRNA genes from the mitochondrion and plastid hinders the sensitive ...
Luyang Song, Kabin Xie
doaj   +1 more source

Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets

open access: yesData in Brief, 2021
The potential for aerobic and anaerobic microbial natural attenuation of PCBs in freshwater sediments is described by PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets generated, in duplicate, from 27 sediment samples ...
Timothy E. Mattes   +6 more
doaj   +1 more source

Combination of 16S rRNA and procalcitonin in diagnosis of neonatal clinically suspected sepsis

open access: yesJournal of International Medical Research, 2020
Objective To investigate the application of 16S rRNA in diagnosing patients with neonatal sepsis. Methods We studied 60 consecutive neonatal patients with clinically suspected sepsis and 20 non-infective cases as controls.
Renqiang Yu   +4 more
doaj   +1 more source

Linking 16S rRNA Gene Classification to amoA Gene Taxonomy Reveals Environmental Distribution of Ammonia-Oxidizing Archaeal Clades in Peatland Soils

open access: yesmSystems, 2021
A highly resolved taxonomy for ammonia-oxidizing archaea (AOA) based on the alpha subunit of ammonia monooxygenase (amoA) was recently established, which uncovered novel environmental patterns of AOA, challenging previous generalizations.
Haitao Wang   +6 more
doaj   +1 more source

Diagnostic Yield and Impact on Antimicrobial Management of 16S rRNA Testing of Clinical Specimens

open access: yesMicrobiology Spectrum, 2022
16S rRNA gene sequencing is increasingly used in clinical practice for bacterial identification of clinical specimens. However, studies on its applicability to direct clinical specimens are limited. Here, we studied the diagnostic yield and impact of 16S
Pruke Eamsakulrat   +2 more
doaj   +1 more source

Reconstructing 16S rRNA genes in metagenomic data [PDF]

open access: yesBioinformatics, 2015
Abstract Metagenomic data, which contains sequenced DNA reads of uncultured microbial species from environmental samples, provide a unique opportunity to thoroughly analyze microbial species that have never been identified before. Reconstructing 16S ribosomal RNA, a phylogenetic marker gene, is usually required to analyze the composition
James R. Cole   +3 more
openaire   +2 more sources

Molecular cloning of the rRNA genes of Prevotella bryantii B14

open access: yesActa Agriculturae Slovenica, 2000
Parts of the rRNA operons of anaerobic rumen bacteria Prevotella bryantii B14 were cloned. 350 colonies carrying pBluescriptII with HindIII DNA fragments were screened with 16S rDNA specific probe and six positive clones were obtained.
Matjaž PETERKA, Gorazd AVGUŠTIN
doaj   +1 more source

Concatenated 16S rRNA sequence analysis improves bacterial taxonomy [version 3; peer review: 2 approved]

open access: yesF1000Research, 2023
Background: Microscopic, biochemical, molecular, and computer-based approaches are extensively used to identify and classify bacterial populations.
Bobby Paul
doaj   +1 more source

Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies

open access: yesInternational Journal of Systematic and Evolutionary Microbiology, 2017
The recent advent of DNA sequencing technologies facilitates the use of genome sequencing data that provide means for more informative and precise classification and identification of members of the Bacteria and Archaea.
Seok-Hwan Yoon   +6 more
semanticscholar   +1 more source

Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences

open access: yesNature Biotechnology, 2013
Profiling phylogenetic marker genes, such as the 16S rRNA gene, is a key tool for studies of microbial communities but does not provide direct evidence of a community's functional capabilities.
M. Langille   +11 more
semanticscholar   +1 more source

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