Results 271 to 280 of about 2,798,395 (304)
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Copy number of the 16S rRNA gene in Coxiella burnetii
European Journal of Epidemiology, 1997Coxiella burnetii is an obligate intracellular bacterium with a doubling time of 5-7 hours. Chromosomal DNA from C. burnetii was digested with various restriction enzymes previously determined to not cut within the genomic 16S rRNA gene, or with a combination of these noncutting enzymes in conjunction with AflIII, a restriction enzyme that cuts twice ...
G, Afseth, L P, Mallavia
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Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosa
The Lancet, 2003Bacteria develop resistance to aminoglycosides by producing aminoglycoside-modifying enzymes such as acetyltransferase, phosphorylase, and adenyltransferase. These enzymes, however, cannot confer consistent resistance to various aminoglycosides because of their substrate specificity.
Keiko, Yokoyama +8 more
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Intravaginal microbial flora by the 16S rRNA gene sequencing
American Journal of Obstetrics and Gynecology, 2011Conventional diagnosis of bacterial vaginosis contains some controversial points. To understand accurately the relationship between clinical stages and the microbiotas, the intravaginal microbial flora was analyzed by the clone library method.Vaginal fluid samples from 31 patients were examined. Lactobacillary grade, Nugent score, culture-based method,
Kazuaki, Yoshimura +6 more
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Diversity of 16S rRNA gene, ITS region and aclB gene of the Aquificales
Extremophiles, 2006The Aquificales are prevalent members of the microbial communities inhabiting many marine and terrestrial hydrothermal systems. Numerous new strains were obtained from deep-sea and terrestrial hydrothermal systems. In order to resolve the phylogenetic relationships within this group, three different phylogenetic datasets were used, namely the 16S rRNA ...
I, Ferrera +5 more
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Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of E. Coli
Cell, 1976We have identified genes for tRNAGLU/2 on the transducing phages o80d3ilvsu7+ (see Ohtsubo et al., 1974) and lambdarifd18 (Kirschbaum and Konrad, 1973), and a gene for tRNAlle/1 on the transducing phage o80rifr (Konrad, Kirschbaum, and Austin, 1973). All these phages have previously been shown to carry genes for rRNA (Ohtsubo et al., 1974; Lindahl et ...
E, Lund +5 more
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16S rRNA Gene Probes for Deinococcus species
Systematic and Applied Microbiology, 1996Summary Oligonucleotide probes specific for members of the unusually radiation-resistant genus Deinococcus were designed and tested. Putative probes were identified by selecting 16S rRNA gene sequences unique to Deinococcus radiodurans UWO 298 (ATCC 35073).
Mark G. Wise +2 more
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Nested PCR and RFLP Analysis Based on the 16S rRNA Gene
2012Current phytoplasma detection and identification methods are primarily based on nested polymerase chain reaction followed by restriction fragment length polymorphism analysis and gel electrophoresis. These methods can potentially detect and differentiate all phytoplasmas including those previously not described.
DUDUK, BOJAN +3 more
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Molecular and General Genetics MGG, 1986
In order to study the organization of the ribosomal RNA genes of Mycoplasma hyopneumoniae the rRNA genes were cloned in phage vectors lambda EMBL3 and lambda EMBL4. By subcloning the restriction fragments into various plasmids and analysing the resulting clones by Southern and Northern blot hybridization, a restriction map of the rRNA genes was ...
C, Taschke +3 more
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In order to study the organization of the ribosomal RNA genes of Mycoplasma hyopneumoniae the rRNA genes were cloned in phage vectors lambda EMBL3 and lambda EMBL4. By subcloning the restriction fragments into various plasmids and analysing the resulting clones by Southern and Northern blot hybridization, a restriction map of the rRNA genes was ...
C, Taschke +3 more
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Targeted 16S rRNA Gene Sequencing for Water Samples
The choice of variable region to amplify in 16S rRNA-targeted amplicon sequencing has long been a matter of debate. Here, we describe a method for sequencing multiple variable regions with the Ion 16S Metagenomics kit, which amplifies six different amplicons covering seven 16S variable regions.Mélanie Pimenta +2 more
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