Results 31 to 40 of about 505,000 (387)

On the limits of 16S rRNA gene-based metagenome prediction and functional profiling

open access: yesbioRxiv, 2023
Molecular profiling techniques such as metagenomics, metatranscriptomics or metabolomics offer important insights into the functional diversity of the microbiome. In contrast, 16S rRNA gene sequencing, a widespread and cost-effective technique to measure
M. Matchado   +7 more
semanticscholar   +1 more source

Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies

open access: yesInternational Journal of Systematic and Evolutionary Microbiology, 2017
The recent advent of DNA sequencing technologies facilitates the use of genome sequencing data that provide means for more informative and precise classification and identification of members of the Bacteria and Archaea.
Seok-Hwan Yoon   +6 more
semanticscholar   +1 more source

Interpretations of Environmental Microbial Community Studies Are Biased by the Selected 16S rRNA (Gene) Amplicon Sequencing Pipeline

open access: yesFrontiers in Microbiology, 2020
One of the major methods to identify microbial community composition, to unravel microbial population dynamics, and to explore microbial diversity in environmental samples is high-throughput DNA- or RNA-based 16S rRNA (gene) amplicon sequencing in ...
D. Straub   +5 more
semanticscholar   +1 more source

Performance of Conventional Urine Culture Compared to 16S rRNA Gene Amplicon Sequencing in Children with Suspected Urinary Tract Infection

open access: yesMicrobiology Spectrum, 2021
Because some organisms causing urinary tract infection (UTI) may be difficult to culture, examination of bacterial gene sequences in the urine may provide a more accurate view of bacteria present during a UTI.
Christopher W. Marshall   +5 more
doaj   +1 more source

Yersinia spp. Identification Using Copy Diversity in the Chromosomal 16S rRNA Gene Sequence. [PDF]

open access: yesPLoS ONE, 2016
API 20E strip test, the standard for Enterobacteriaceae identification, is not sufficient to discriminate some Yersinia species for some unstable biochemical reactions and the same biochemical profile presented in some species, e.g.
Huijing Hao   +9 more
doaj   +1 more source

Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB

open access: yesApplied and Environmental Microbiology, 2006
A 16S rRNA gene database (http://greengenes.lbl.gov ) addresses limitations of public repositories by providing chimera screening, standard alignment, and taxonomic classification using multiple published taxonomies.
T. DeSantis   +9 more
semanticscholar   +1 more source

Molecular cloning of the rRNA genes of Prevotella bryantii B14

open access: yesActa Agriculturae Slovenica, 2000
Parts of the rRNA operons of anaerobic rumen bacteria Prevotella bryantii B14 were cloned. 350 colonies carrying pBluescriptII with HindIII DNA fragments were screened with 16S rDNA specific probe and six positive clones were obtained.
Matjaž PETERKA, Gorazd AVGUŠTIN
doaj   +1 more source

Strategy for the identification of micro-organisms producing food and feed products : bacteria producing food enzymes as study case [PDF]

open access: yes, 2020
Recent European regulations require safety assessments of food enzymes (FE) before their commercialization. FE are mainly produced by micro-organisms, whose viable strains nor associated DNA can be present in the final products.
De Keersmaecker, Sigrid C. J.   +8 more
core   +2 more sources

Microbial signatures in amniotic fluid at preterm birth and association with bronchopulmonary dysplasia

open access: yesRespiratory Research, 2023
Background Microbiome dysbiosis can have long-lasting effects on our health and induce the development of various diseases. Bronchopulmonary dysplasia (BPD) is a multifactorial disease with pre- and postnatal origins including intra-amniotic infection as
Birte Staude   +7 more
doaj   +1 more source

Effect of Intragenomic Sequence Heterogeneity among Multiple 16S rRNA Genes on Species Identification of Elizabethkingia

open access: yesMicrobiology Spectrum, 2022
Accurate identification of Elizabethkingia species mostly requires the use of molecular techniques, and 16S rRNA gene sequencing is generally considered the method of choice.
Jiun-Nong Lin   +7 more
doaj   +1 more source

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