Results 91 to 100 of about 246,967 (290)

Species-Specific Identification of Campylobacters by Partial 16S rRNA Gene Sequencing [PDF]

Journal of Clinical Microbiology, 2003
ABSTRACT Species-specific identification of campylobacters is problematic, primarily due to the absence of suitable biochemical assays and the existence of atypical strains. 16S rRNA gene (16S rDNA)-based identification of bacteria offers a possible alternative when phenotypic tests fail.
Gregor, Gorkiewicz, Gebhard, Feierl, Caroline, Schober, Franz, Dieber, Josef, Köfer, Rudolf, Zechner, Ellen L, Zechner   +6 more
openaire   +2 more sources

High‐Throughput 3D Matrigel‐based Droplet Microfluidics for Single‐Cell Function‐to‐Omics Analysis of Cytotoxic Immune Cells in Solid Tumor Interactions

Advanced Materials Technologies, EarlyView.
A 3D Matrigel‐based droplet microfluidics platform that allows physiologically relevant analysis of single immune‐tumor interactions in solid tumors. By integrating real‐time functional imaging with fluorescence‐activated droplet sorting and RNA sequencing, the system links immune cytotoxic behavior to transcriptomic profiles, providing a scalable ...
Christina Sharkey, Harry Akligoh, Michael Finocchiaro, Abhishek Srihari Rampelli, Zongwei Wang, Aria F. Olumi, Tania Konry   +6 more
wiley   +1 more source

Taxonomic classification of bacterial 16S rRNA genes using short sequencing reads: evaluation of effective study designs. [PDF]

PLoS ONE, 2013
Massively parallel high throughput sequencing technologies allow us to interrogate the microbial composition of biological samples at unprecedented resolution.
Orna Mizrahi-Man, Emily R Davenport, Yoav Gilad   +2 more
doaj   +1 more source

Ovine pedomics : the first study of the ovine foot 16S rRNA-based microbiome [PDF]

, 2011
We report the first study of the bacterial microbiome of ovine interdigital skin based on 16S rRNA by pyrosequencing and conventional cloning with Sanger-sequencing.
A Dhawi, AL Cogen, AP Escayg, Atiya Ul-Hassan, BF Langworth, BH Jost, Brian B Oakley, C Lozupone, Claire L Russell, DA Murdoch, DJ Lane, DJ Stewart, DL Emery, DS Roberts, E Pruesse, E Zaura, EA Grice, EA Grice, Elizabeth MH Wellington, G Bennett, G Conrads, G Jones, G Muyzer, G Sayers, GC Baker, GR Carter, Graham F Medley, H Ulbegi-Mohyla, H Zhou, I Demirkan, IH Davies, J Kaler, J Kaler, J Kaler, JA Frank, Jasmeet Kaler, JC Chin, JP Galkiewicz, JR Egerton, K Klitgaard, Laura E Green, LE Green, Leo A Calvo-Bado, LJ Moore, Luci Witcomb, M Chrino-Trejo, MC Liu, Miloslav Kocur, NJ Evans, NJ Evans, PA Lawson, PD Schloss, PD Schloss, RD Naylor, RE Ley, RJ Collighan, Rose Grogono-Thomas, RS Howell-Jones, S La Fontaine, S Lavín, S Piriz Duran, S Tadepalli, Scot E Dowd, SE Dowd, SJ Billington, TG Nagaraja, TM Skerman, TZ DeSantis, TZ DeSantis Jr, Vicky Bateman, W Li, W Ludwing, WE Kloos, WG Weisburg, WIB Beveridge, William Gaze   +75 more
core   +2 more sources

Dual‐Functional Ingestible Passive Capsules for High‐Throughput Intestinal Sampling with Sealed Containment and Targeted Drug Delivery

Advanced Robotics Research, EarlyView.
To address challenges in high‐throughput intestinal sampling with sealed containment and target drug delivery, we developed a dual‐functional ingestible passive capsule with a dual‐triggered control system based on pH‐response and mechanical actuation.
Libing Huang, Houyu Ling, Xizong Chen, Yufei Duan, Yu Liu, Shuntian Cai, Yandan Ren, Wenlong Li, Lei Ren, Miao Wang   +9 more
wiley   +1 more source

Ion torrent-based transcriptional assessment of a Corynebacterium pseudotuberculosis equi strain reveals denaturing high-performance liquid chromatography a promising rRNA depletion method [PDF]

, 2013
Corynebacterium pseudotuberculosis equi is a Gram-positive pathogenic bacterium which affects a variety of hosts. Besides the great economic losses it causes to horse-breeders, this organism is also known to be an important infectious agent to cattle and
Azarani, Azarani, Baird, Barakat, Biberstein, Braverman, Bustin, Chen, Chen, Colbert, Conesa, Efstratiou, Gaspar, Hantke, Jones, Jünemann, Lukashin, Martin, Meldrum, Muto, Nguyen, Nolden, Pacheco, Papp-Wallace, Patel, Paton, Pfaffl, Piddington, Pinho, Pinto, Ramos, Ramos, Richardson, Ruiz, Sambrook, Schulz, Sorek, Spier, Swiercz, Trost, Vliet, Weinberg, Westermann, Wong, Zerbino   +44 more
core   +1 more source

Phylogenetic Analysis of Erwinia Species Based on 16S rRNA Gene Sequences [PDF]

International Journal of Systematic Bacteriology, 1997
The phylogenetic relationships of the type strains of 16 Erwinia species were investigated by performing a comparative analysis of the sequences of the 16S rRNA genes of these organisms. The sequence data were analyzed by the neighbor-joining method, and each branch was supported by moderate bootstrap values. The phylogenetic tree and sequence analyses
S W, Kwon, S J, Go, H W, Kang, J C, Ryu, J K, Jo   +4 more
openaire   +2 more sources

Microbial Contribution to Soiling and Its Impact on Photovoltaic Module Soiling in Arid Zones of the Atacama Desert

Advanced Sustainable Systems, EarlyView.
Microorganisms colonizing photovoltaic surfaces in the Atacama desert form biofilms that enhance particle adhesion and reduce energy yield. This study identifies UV‐resistant bacteria and carotenoid‐producing strains that interfere with PV performance.
Douglas Olivares, Ricardo Ortíz, Luis Rojas‐Araya, María Teresa Mata, Felipe Galleguillos, Jaime Llanos, Carlos Portillo, Valentina Escobar, Aitor Marzo   +8 more
wiley   +1 more source

Bacteria and Fungi Synergistically Reprogram Flavonoid Metabolites in the Pericarp of Citrus Reticulata 'Chachi' During Storage

Advanced Science, EarlyView.
The authors employed widely targeted metabolomics, microbial amplicon sequencing, and fermentation assays to investigate the microbiome 's influence on PCRC 's (pericarp of Citrus reticulata ' Chachi ') flavonoid profile over 0–19 years of storage. Based on the correlation analysis, solid‐state and liquid‐state fermentation, and catalytic activity ...
Jianmu Su, Sisheng Zhang, Lidi Liang, Haiyi Bai, Mei Bai, Hanjun He, Yayu Wang, Huan Liu, Xiangxiu Liang, Yu Sun, Hong Wu   +10 more
wiley   +1 more source

The gyrB gene is a useful phylogenetic marker for exploring the diversity of flavobacterium strains isolated from terrestrial and aquatic habitats in Antarctica [PDF]

, 2011
Within the phylum Bacteroidetes, the gyrB gene, encoding for the B subunit of the DNA gyrase, has been used as phylogenetic marker for several genera closely related to Flavobacterium.
Peeters, Karolien, Willems, Anne
core   +1 more source