Results 91 to 100 of about 245,723 (333)

Rapid and reliable species-level identification from clinical samples using 16S rRNA gene nanopore sequencing analysis

Scientific Reports
The detection and identification of bacterial species in clinical samples are crucial for patient management and antibiotic treatment. When culture-based identification methods fail, 16S rRNA gene next-generation sequencing (NGS) serves as a valuable ...
Marco J. R. Ivens, Sergio Kamminga, Kawtar Benchamach, Chaimae Akile, Els Wessels, Eric C. J. Claas, Stefan A. Boers   +6 more
doaj   +1 more source

Limitations of 16S rRNA Gene Sequencing to Characterize Lactobacillus Species in the Upper Genital Tract

Frontiers in Cell and Developmental Biology, 2021
The endometrial cavity is an upper genital tract site previously thought as sterile, however, advances in culture-independent, next-generation sequencing technology have revealed that this low-biomass site harbors a rich microbial community which ...
Jessica L. O’Callaghan, Jessica L. O’Callaghan, Dana Willner, Dana Willner, Melissa Buttini, Flavia Huygens, Flavia Huygens, Elise S. Pelzer, Elise S. Pelzer   +8 more
doaj   +1 more source

Capturing Greater 16S rRNA Gene Sequence Diversity within the Domain Bacteria [PDF]

Applied and Environmental Microbiology, 2012
ABSTRACT A large proportion of “universal” 16S PCR primers lack sequence homology to many of the “candidate” divisions, severely limiting bacterial diversity assessments. We designed a primer set that offers a 50% increase in silico in coverage of the domain Bacteria over the ...
T, Winsley, J M, van Dorst, M V, Brown, B C, Ferrari   +3 more
openaire   +2 more sources

Membrane Vesicles from Lacticaseibacillus Casei BL23 Exhibit Antimicrobial Activity Against Escherichia coli and Immunostimulatory Effects on Human Peripheral Blood Mononuclear Cells

Advanced Healthcare Materials, EarlyView.
Membrane vesicles derived from the probiotic Lacticaseibacillus casei BL23 demonstrate antimicrobial properties against Escherichia coli and a potential biological effect in improving the overall survival of C. elegans infected with Pseudomonas aeruginosa. These vesicles stimulated immune responses in primary cells without causing toxicity. Our results
Cecilia L. D'Antoni, Leila Pourtalebi Jahromi, Lorenzo Sana, Ana Paula Domínguez Rubio, Maja Dorfner, Jennifer Munkert, Heike Danzer, Philipp Arnold, Mikhail Lebedev, Esther Zanin, Oscar E. Pérez, Gregor Fuhrmann   +11 more
wiley   +1 more source

Ovine pedomics : the first study of the ovine foot 16S rRNA-based microbiome [PDF]

, 2011
We report the first study of the bacterial microbiome of ovine interdigital skin based on 16S rRNA by pyrosequencing and conventional cloning with Sanger-sequencing.
A Dhawi, AL Cogen, AP Escayg, Atiya Ul-Hassan, BF Langworth, BH Jost, Brian B Oakley, C Lozupone, Claire L Russell, DA Murdoch, DJ Lane, DJ Stewart, DL Emery, DS Roberts, E Pruesse, E Zaura, EA Grice, EA Grice, Elizabeth MH Wellington, G Bennett, G Conrads, G Jones, G Muyzer, G Sayers, GC Baker, GR Carter, Graham F Medley, H Ulbegi-Mohyla, H Zhou, I Demirkan, IH Davies, J Kaler, J Kaler, J Kaler, JA Frank, Jasmeet Kaler, JC Chin, JP Galkiewicz, JR Egerton, K Klitgaard, Laura E Green, LE Green, Leo A Calvo-Bado, LJ Moore, Luci Witcomb, M Chrino-Trejo, MC Liu, Miloslav Kocur, NJ Evans, NJ Evans, PA Lawson, PD Schloss, PD Schloss, RD Naylor, RE Ley, RJ Collighan, Rose Grogono-Thomas, RS Howell-Jones, S La Fontaine, S Lavín, S Piriz Duran, S Tadepalli, Scot E Dowd, SE Dowd, SJ Billington, TG Nagaraja, TM Skerman, TZ DeSantis, TZ DeSantis Jr, Vicky Bateman, W Li, W Ludwing, WE Kloos, WG Weisburg, WIB Beveridge, William Gaze   +75 more
core   +2 more sources

New Insights into Atonic Postpartum Hemorrhage: Animal Model Construction Based on Placental Nanodelivery Systems

Advanced Healthcare Materials, EarlyView.
This study develops a placenta‐targeted nanodelivery system co‐loading HMGB1 protein and the NLRP3 agonist nigericin to establish an animal model of atonic postpartum hemorrhage. The model accurately recapitulates clinical phenotypes, including prolonged labor and uterine contractility dysfunction, while revealing inflammatory activation in placental ...
Jiangxue Qu, Ruiqi Wu, Hai Jiang, Lian Chen, Yingying Tian, Shuai Zeng, Tongmin Liu, Cheng Zhao, Huifeng Shi, Xiaolong Liang, Yangyu Zhao   +10 more
wiley   +1 more source

Species-Specific Identification of Campylobacters by Partial 16S rRNA Gene Sequencing [PDF]

Journal of Clinical Microbiology, 2003
ABSTRACT Species-specific identification of campylobacters is problematic, primarily due to the absence of suitable biochemical assays and the existence of atypical strains. 16S rRNA gene (16S rDNA)-based identification of bacteria offers a possible alternative when phenotypic tests fail.
Gregor, Gorkiewicz, Gebhard, Feierl, Caroline, Schober, Franz, Dieber, Josef, Köfer, Rudolf, Zechner, Ellen L, Zechner   +6 more
openaire   +2 more sources

Targeting the ARRDC3–DRP1 Axis via hUMSC‐Derived Exosomal CRYAB for Neuroprotection in Cerebral Ischemia/Reperfusion Injury

Advanced Healthcare Materials, EarlyView.
Intranasally administered hUMSC‐derived exosomes modulate the CRYAB–ARRDC3–Drp1 axis, alleviating mitochondrial dysfunction and ferroptosis, enhancing neuronal survival, reducing oxidative stress, and promoting functional recovery in ischemia‐reperfusion injury, offering a promising therapeutic strategy for ischemic stroke.
Rong ji, Zengyu Zhang, Zhuohang Liu, Kaicheng Yang, Xueyu Mao, Min Chu, Yong Wang, Jing Zhao   +7 more
wiley   +1 more source

Taxonomic classification of bacterial 16S rRNA genes using short sequencing reads: evaluation of effective study designs. [PDF]

PLoS ONE, 2013
Massively parallel high throughput sequencing technologies allow us to interrogate the microbial composition of biological samples at unprecedented resolution.
Orna Mizrahi-Man, Emily R Davenport, Yoav Gilad   +2 more
doaj   +1 more source

3D‐Printed Gastrointestinal Stents: In Vivo Evaluation in a Swine Small Bowel Perforation Model

Advanced Healthcare Materials, EarlyView.
Gastrointestinal fistulae and perforations can lead to severe complications including sepsis and patient death. In this work, the efficacy of 3D‐printed gastrointestinal stents composed of poly‐lactic‐acid (PLA) was evaluated in an in vivo swine model.
Gweniviere Capron, Parinaz Fathi, Tor Wolf Jensen, Derek J. Milner, André J. van der Vlies, Regan L. Moody, Elizabeth A. Bangert, Blair Rowitz, Dipanjan Pan, Matthew B. Wheeler   +9 more
wiley   +1 more source