Results 111 to 120 of about 3,149,654 (297)

Using short-read 16S rRNA sequencing of multiple variable regions to generate high-quality results to a species level

Frontiers in Bioinformatics
IntroductionShort-read amplicon sequencing studies have typically focused on 1-2 variable regions of the 16S rRNA gene. Species-level resolution is limited in these studies, as each variable region enables the characterisation of a different subsection ...
Amy S. Graham, Amy S. Graham, Fadheela Patel, Francesca Little, Andre van der Kouwe, Andre van der Kouwe, Mamadou Kaba, Martha J. Holmes, Martha J. Holmes, Martha J. Holmes, Martha J. Holmes   +10 more
doaj   +1 more source

Lactiplantibacillus Plantarum GUANKE Alleviates Allergic Asthma by Restricting Monocyte‐to‐DC Differentiation Via Microbiota‐Associated IAA

Advanced Science, EarlyView.
Lactiplantibacillus plantarum GUANKE remodels the indigenous gut microbiota and is associated with microbiota‐dependent restoration of circulating IAA. IAA treatment attenuates JAK3‐STAT3 and Jag1‐Notch4‐Hey1 signaling and reduces CX3CR1+Ly6C− monocytic precursor generation and pulmonary CD11b+ DC accumulation, together with reduced Th2 inflammation ...
Yujia He, Jielan Mi, Zhiping Zhao, Xiao Zhang, Zhihan Yang, Kun Yue, Yuanming Huang, Liqiong Song, Xiaoting Wen, Jianguo Xu, Zhihong Ren   +10 more
wiley   +1 more source

Molecular biological methods for studying the gut microbiota : the EU human gut flora project [PDF]

, 2002
Seven European laboratories co-operated in a joint project (FAIR CT97-3035) to develop, refine and apply molecular methods towards facilitating elucidation of the complex composition of the human intestinal microflora and to devise robust methodologies ...
Vos, W.M., de, Collins, M.D., Welling, G.W., Loo, J., van, Blaut, M., Dore, J.   +5 more
core   +1 more source

Polychip‐A High‐Throughput Droplet Microfluidics Platform for Interrogating Microbial Interactions

Advanced Science, EarlyView.
Polychip, a fully integrated droplet microfluidics platform, enables high‐throughput, single‐cell resolution screening of polymicrobial interactions. By seamlessly combining six automated microfluidics operations on a single chip, the system accelerates antimicrobial discovery by 11 to 14 times compared to traditional robotic methods.
Jeong Jae Han, Adrian Ryan Guzman, Aifen Zhou, Han Zhang, Rohit Gupte, Haemin Jung, Kaylee Delgado, Sini Skariah, Ali Sultan, Arul Jayaraman, Paul de Figueiredo, Arum Han   +11 more
wiley   +1 more source

Evaluation of the colorimetric VITEK 2 card for identification of gram-negative nonfermentative rods: comparison to 16S rRNA gene sequencing

, 2007
Ninety strains of a collection of well-identified clinical isolates of gram-negative nonfermentative rods collected over a period of 5 years were evaluated using the new colorimetric VITEK 2 card.
Böttger, E C, Bosshard, P P, Zbinden, R, Zbinden, A   +3 more
core   +1 more source

Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification. [PDF]

, 2016
BACKGROUND: Currently, bacterial 16S rRNA gene analyses are based on sequencing of individual variable regions of the 16S rRNA gene (Kozich, et al Appl Environ Microbiol 79:5112-5120, 2013).This short read approach can introduce biases. Thus, full-length
Suzanna C. Francis, Josef Wagner, Hilary P. Browne, Francis, Suzanna C, Trevor D. Lawley, Browne, Hilary P, Lawley, Trevor D, Coupland, Paul, Parkhill, Julian, Paul Coupland, Wagner, Josef, Julian Parkhill   +11 more
core   +3 more sources

Gut microbiota-16S rRNA gene sequencing data

, 2022
To explore whether liraglutide could improve dyslipidemia by modulating the gut microbiota, the C57BL/6 mice were fed ahigh-fat diet to establish an animal model of dyslipidemia, and then administered with liraglutide or normal saline. After intervention
Li Zhao (14300624)
core   +1 more source

RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops

Advanced Science, EarlyView.
This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Xiong Zhang, Yupo Wu, Jiachang Hou, Lixia Gao, Longqi Pan, Cuicui Shen, Chuanji Zhao, Feng Gao, Yi Zhang, Li Ren, Xiaoping Fang, Xiaoling Dun, Shengyi Liu, Lijiang Liu   +13 more
wiley   +1 more source

Preliminary detection of haemoplasma in Thai cat blood samples using universal primers: identifying ‘ Mycoplasma haemominutum’ and closely related species

Journal of Feline Medicine and Surgery
Objectives This study examined feline haemoplasmas ( Mycoplasma haemofelis , ‘ Candidatus Mycoplasma haemominutum’ [ C Mhm] and ‘ Candidatus Mycoplasma turicensis’) infecting Thai domestic cats, using the 16S and 23S rRNA genes as genetic markers ...
Thuong Thi Huyen Bui, Kritsada Thongmeesee, Wittawat Wechtaisong, Sonthaya Tiawsirisup   +3 more
doaj   +1 more source

Targeted Delivery of Indole‐3‐Pyruvic Acid Suppresses Macrophage Ferroptosis to Enhance CD8+ T Cell‐Mediated Immunotherapy Response in Bladder Cancer

Advanced Science, EarlyView.
A microbiota‐derived metabolite, indole‐3‐pyruvic acid, suppresses macrophage ferroptosis through the AHR–NF‐κB–SLC7A11 axis. This preserves CD8+ T cell function in bladder cancer. Macrophage‐targeted nanoparticles enhance indole‐3‐pyruvic acid delivery and overcome resistance to PD‐1 blockade.
Jianwen Lao, Xinhao Yuan, Shuai Liang, Kai Deng, Daqin Wu, Longhao Xu, Kun Zheng, Yi Lin, Peicong Cai, Haoran Zheng, Junyu Chen, Mingli Luo, Yan Chen, Xiong Chen, Chunhui Wang, Wenlong Zhong   +15 more
wiley   +1 more source