Results 71 to 80 of about 250,877 (332)

Effect of Intragenomic Sequence Heterogeneity among Multiple 16S rRNA Genes on Species Identification of Elizabethkingia

Microbiology Spectrum, 2022
Accurate identification of Elizabethkingia species mostly requires the use of molecular techniques, and 16S rRNA gene sequencing is generally considered the method of choice.
Jiun-Nong Lin, Chung-Hsu Lai, Shang-Yi Lin, Ching-Chi Lee, Nan-Yao Lee, Po-Yu Liu, Chih-Hui Yang, Yi-Han Huang   +7 more
doaj   +1 more source

Bacteria isolated from Bengal cat (Felis catus × Prionailurus bengalensis) anal sac secretions produce volatile compounds potentially associated with animal signaling. [PDF]

, 2019
In social animals, scent secretions and marking behaviors play critical roles in communication, including intraspecific signals, such as identifying individuals and group membership, as well as interspecific signaling.
Cho, Adrienne W, Coil, David A, Davis, Cristina E, Eisen, Jonathan A, Ganz, Holly H, Jospin, Guillaume, McCartney, Mitchell M, Yamaguchi, Mei S, Zaw, Thant H   +8 more
core   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Application of MinION Amplicon Sequencing to Buccal Swab Samples for Improving Resolution and Throughput of Rumen Microbiota Analysis

Frontiers in Microbiology, 2022
The Illumina MiSeq platform has been widely used as a standard method for studying the rumen microbiota. However, the low resolution of taxonomic identification is the only disadvantage of MiSeq amplicon sequencing, as it targets a part of the 16S rRNA ...
Hiroto Miura, Masayuki Takeda, Megumi Yamaguchi, Yoshihisa Ohtani, Go Endo, Yasuhisa Masuda, Kaede Ito, Yoshio Nagura, Kunihiro Iwashita, Tomohiro Mitani, Yutaka Suzuki, Yasuo Kobayashi, Satoshi Koike   +12 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Erwinia billingiae as Unusual Cause of Septic Arthritis, France, 2017

Emerging Infectious Diseases, 2019
In 2017 in France, we treated a patient with knee septic arthritis caused by Erwinia billingiae after trauma involving a palm tree. This rare pathogen could only be identified through 16S rRNA gene sequencing. For bacterial infections after injuries with
Isabelle Bonnet, Baptiste Bozzi, Eric Fourniols, Stéphane Mitrovic, Olivia Soulier-Escrihuela, Florence Brossier, Wladimir Sougakoff, Jérôme Robert, Stéphane Jauréguiberry, Alexandra Aubry   +9 more
doaj   +1 more source

Ecological and Genomic Attributes of Novel Bacterial Taxa That Thrive in Subsurface Soil Horizons. [PDF]

, 2019
While most bacterial and archaeal taxa living in surface soils remain undescribed, this problem is exacerbated in deeper soils, owing to the unique oligotrophic conditions found in the subsurface.
Arogyaswamy, Keshav, Aronson, Emma L, Billings, Sharon A, Botthoff, Jon K, Brewer, Tess E, Campbell, Ashley N, Dove, Nicholas C, Fairbanks, Dawson, Fierer, Noah, Gallery, Rachel E, Hart, Stephen C, Kaye, Jason, King, Gary, Logan, Geoffrey, Lohse, Kathleen A, Maltz, Mia R, Mayorga, Emilio, O'Neill, Caitlin, Owens, Sarah M, Packman, Aaron, Pett-Ridge, Jennifer, Plante, Alain F, Richter, Daniel D, Silver, Whendee L, Yang, Wendy H   +24 more
core   +2 more sources

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Amniotic fluid from healthy term pregnancies does not harbor a detectable microbial community [PDF]

, 2018
Recent studies have conflicting data regarding the presence of intra-amniotic microbiota. Viral communities are increasingly recognized as important although overlooked components of the human microbiota.
Holtz, Lori R, Lim, Efrem S, Rodriquez, Cynthia   +2 more
core   +2 more sources

Mobile resistome of human gut and pathogen drives anthropogenic bloom of antibiotic resistance [PDF]

, 2020
BACKGROUND:The impact of human activities on the environmental resistome has been documented in many studies, but there remains the controversial question of whether the increased antibiotic resistance observed in anthropogenically impacted environments ...
Bu, Ji-Hye, Cha, Chang-Jun, Cha, Ju-Hee, Hwang, Eun-Mi, Kim, Dae-Wi, Kim, Yong-Seok, Lee, Do-Hoon, Lee, Kihyun, Quince, Christopher, Seong, Hoon Je, Sul, Woo Jun, Thawng, Cung Nawl, Wellington, Elizabeth M. H.   +12 more
core   +1 more source