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Electrochemical biosensing of 16s rRNA gene sequence of Enterococcus faecalis
Biosensors and Bioelectronics, 2019Some of microorganisms are potential pathogens that can be infectious agents under some circumstances, and development of new detection methods of the pathogens is of high interest. In the present study, an Enterococcus faecalis (E. faecalis) DNA biosensor (ef-biosensor) was fabricated to quantify the bacterium genome. A specific E.
R, Nazari-Vanani +4 more
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Targeted 16S rRNA Gene Sequencing for Water Samples
The choice of variable region to amplify in 16S rRNA-targeted amplicon sequencing has long been a matter of debate. Here, we describe a method for sequencing multiple variable regions with the Ion 16S Metagenomics kit, which amplifies six different amplicons covering seven 16S variable regions.Mélanie Pimenta +2 more
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16S rRNA gene sequencing for bacterial pathogen identification in the clinical laboratory
Molecular Diagnosis, 2001For many years, sequencing of the 16S ribosomal RNA (rRNA) gene has served as an important tool for determining phylogenetic relationships between bacteria. The features of this molecular target that make it a useful phylogenetic tool also make it useful for bacterial detection and identification in the clinical laboratory. Sequence analysis of the 16S
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Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates
Veterinary Microbiology, 2011Ureaplasma diversum infection in bulls may result in seminal vesiculitis, balanoposthitis and alterations in spermatozoids. In cows, it can cause placentitis, fetal alveolitis, abortion and the birth of weak calves. U. diversum ATCC 49782 (serogroups A), ATCC 49783 (serogroup C) and 34 field isolates were used for this study.
L M, Marques +8 more
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Topographic Map of Gammaproteobacteria using 16S rRNA gene sequence
2007Microbial identification is crucial for the study of infec- tious diseases. The classical method to attribute a specific name to a bacterial isolate to be identified is based on the comparison of morphologic and phenotypic characters to those described for type or typical strains.
Giuseppe Di Fatta +5 more
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[Identification of pathogenic microorganism by sequencing 16S rRNA gene].
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 2015To identify 14 bacteria by sequencing the 16S rRNA gene and establish the basis for clinical application in the future.DNA samples of the 14 bacteria were extracted. The 16S rRNA genes were amplified by PCR and sequenced with common primers. The sequences of the 16S rRNA genes were aligned by online software Blastn in nucleotide database.
Feizhou, Zhu, Liyu, Chen, Hanchun, Chen
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16S rRNA gene sequencing for HIV negative MSM
The fecal samples of 95 HIV negative men who have sex with men (MSM) were collected and subjected to 16S rRNA gene sequecing.openaire +1 more source
Samail Ophiolite 16S rRNA gene amplicon sequencing
This is a central repository for 16S rRNA gene amplicon sequencing data generated from samples of biomass from the Samail Ophiolite, Oman. Further information on processing of this data is available on Github and is archived via Zenodo: Nothaft, D. B., Rempfert, K. R. & Kraus, E. A. danote/Samail_16S_compilation: First release of Samail 16S dataopenaire +1 more source