Results 31 to 40 of about 245,723 (333)

Amplicon Sequence Variants Artificially Split Bacterial Genomes into Separate Clusters

mSphere, 2021
16S rRNA gene sequencing has engendered significant interest in studying microbial communities. There has been tension between trying to classify 16S rRNA gene sequences to increasingly lower taxonomic levels and the reality that those levels were ...
Patrick D. Schloss
doaj   +1 more source

Evaluating the Effect of Dietary Protein-Energy Ratios on Yak Intestinal Microbiota Using High-Throughput 16S rRNA Gene Sequencing. [PDF]

Vet Sci
Zhu Y, Cidan Y, Ali M, Lu S, Javed U, Cisang Z, Gusang D, Danzeng Q, Li K, Basang W.   +9 more
europepmc   +3 more sources

Quantifying dominant bacterial genera detected in metagenomic data from fish eggs and larvae using genus‐specific primers

MicrobiologyOpen, 2022
The goal of this study was to design genus‐specific primers for rapid evaluation of the most abundant bacterial genera identified using amplicon‐based sequencing of the 16S rRNA gene in fish‐related samples and surrounding water. Efficient genus‐specific
Babak Najafpour, Patricia I. S. Pinto, A. V. M. Canario, Deborah M. Power   +3 more
doaj   +1 more source

16S-ITS-23S rRNA operon segment sequencing provides necessary and sufficient conditions for bacterial species-specific identification

Инфекция и иммунитет, 2022
Introduction. Sequencing of the 16S rRNA gene is the predominant method for assessing microbial communities and strain molecular identification. The short reads (2nd generation sequencing)-based technology does not allow analysis beyond the 16S rRNA gene.
O. B. Ogarkov, S. N. Zhdanova, E. A. Orlova, P. A. Khromova, N. L. Belkova, V. V. Sinkov, I. G. Kondratov   +6 more
doaj   +1 more source

Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling

Frontiers in Cellular and Infection Microbiology, 2022
Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies.
Vitor Heidrich, Vitor Heidrich, Lilian T. Inoue, Paula F. Asprino, Fabiana Bettoni, Antonio C. H. Mariotti, Diogo A. Bastos, Denis L. F. Jardim, Marco A. Arap, Anamaria A. Camargo   +9 more
doaj   +1 more source

Progress in the 16S rRNA Gene Sequencing in Forensic Science [PDF]

Fayixue Zazhi, 2018
Forensic microorganism is one of the hotspots of forensic science research. Due to its conservatism and specificity, the 16S rRNA gene is found to be an ideal marker for forensic identification.
SONG Guo-qing, CAO Yu, LI Hui，et al.
doaj   +1 more source

Taxonomy of anaerobic digestion microbiome reveals biases associated with the applied high throughput sequencing strategies [PDF]

, 2018
In the past few years, many studies investigated the anaerobic digestion microbiome by means of 16S rRNA amplicon sequencing. Results obtained from these studies were compared to each other without taking into consideration the followed procedure for ...
Angelidaki, Irini, Campanaro, Stefano, Kougias, Panagiotis G., Treu, Laura, Zhu, Xinyu   +4 more
core   +3 more sources

16S rRNA gene and 16S-23S rRNA gene internal transcribed spacer sequences analysis of the genus Myxococcus [PDF]

FEMS Microbiology Letters, 2008
Phylogenetic relationships of the species belonging to the genus Myxococcus were elucidated based on the sequences of 16S rRNA genes and 16S-23S rRNA gene internal transcribed spacer (ITS) regions. The Myxococcus species were consequently classified into four distinct groups.
Mika Miyashita, Takeshi Sakane, Ken-ichiro Suzuki, Yasuyoshi Nakagawa   +3 more
openaire   +2 more sources

Engineering CRISPR/Cas9 to mitigate abundant host contamination for 16S rRNA gene-based amplicon sequencing

Microbiome, 2020
Background High-throughput sequencing of bacterial 16S rRNA gene (16S-seq) is a useful and common method for studying bacterial community structures. However, contamination of the 16S rRNA genes from the mitochondrion and plastid hinders the sensitive ...
Luyang Song, Kabin Xie
doaj   +1 more source

Concatenated 16S rRNA sequence analysis improves bacterial taxonomy [version 3; peer review: 2 approved]

F1000Research, 2023
Background: Microscopic, biochemical, molecular, and computer-based approaches are extensively used to identify and classify bacterial populations.
Bobby Paul
doaj   +1 more source