Results 51 to 60 of about 253,664 (304)

Identification of bacterial pathogens in sudden unexpected death in infancy and childhood using 16S rRNA gene sequencing

Frontiers in Microbiology, 2023
BackgroundSudden unexpected death in infancy (SUDI) is the most common cause of post-neonatal death in the developed world. Following an extensive investigation, the cause of ~40% of deaths remains unknown.
Lily Gates, Talisa Mistry, Olumide Ogunbiyi, Kerry-Anne Kite, Nigel J. Klein, Neil J. Sebire, Dagmar G. Alber   +6 more
doaj   +1 more source

Generation of Comprehensive Ecosystem-Specific Reference Databases with Species-Level Resolution by High-Throughput Full-Length 16S rRNA Gene Sequencing and Automated Taxonomy Assignment (AutoTax)

mBio, 2019
High-throughput 16S rRNA gene amplicon sequencing is an essential method for studying the diversity and dynamics of microbial communities. However, this method is presently hampered by the lack of high-identity reference sequences for many environmental ...
M. Dueholm, K. S. Andersen, S. McIlroy, J. M. Kristensen, E. Yashiro, S. Karst, M. Albertsen, P. Nielsen   +7 more
semanticscholar   +1 more source

Phylogenetic inference of Coxiella burnetii by 16S rRNA gene sequencing. [PDF]

PLoS ONE, 2017
Coxiella burnetii is a human pathogen that causes the serious zoonotic disease Q fever. It is ubiquitous in the environment and due to its wide host range, long-range dispersal potential and classification as a bioterrorism agent, this microorganism is ...
Heather P McLaughlin, Blake Cherney, Janetta R Hakovirta, Rachael A Priestley, Andrew Conley, Andrew Carter, David Hodge, Segaran P Pillai, Linda M Weigel, Gilbert J Kersh, David Sue   +10 more
doaj   +1 more source

A working model for cytoplasmic assembly of H/ACA snoRNPs

FEBS Letters, EarlyView.
Dyskerin is the component of nuclear H/ACA ribonucleoproteins (RNPs) endowed with pseudouridine synthase catalytic activity. Two isoforms of human dyskerin have been characterized: the abundant Iso1, mainly nuclear, and the shorter Iso3, mainly cytoplasmic but occasionally imported into nuclei.
Alberto Angrisani, Maria Furia
wiley   +1 more source

Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technology

BMC Microbiology
Background Accurate identification of bacterial communities is crucial for research applications, diagnostics, and clinical interventions. Although 16S ribosomal RNA (rRNA) gene sequencing is a widely employed technique for bacterial taxonomic ...
A. Bertolo, E. Valido, Jivko Stoyanov
semanticscholar   +1 more source

Gut Microbiota Profile in Patients with Type 1 Diabetes Based on 16S rRNA Gene Sequencing: A Systematic Review

Disease Markers, 2020
The gut microbiota has been presumed to have a role in the pathogenesis of type 1 diabetes (T1D). Significant changes in the microbial composition of T1D patients have been reported in several case-control studies.
He Zhou, Xue Zhao, Lin Sun, Yujia Liu, You Lv, X. Gang, Guixia Wang   +6 more
semanticscholar   +1 more source

Gut microbiota diversity is prognostic in metastatic hormone receptor‐positive breast cancer patients receiving chemotherapy and immunotherapy

Molecular Oncology, EarlyView.
In this exploratory study, we investigated the relationship between the gut microbiota and outcome in patients with metastatic hormone receptor‐positive breast cancer, treated in a randomized clinical trial with chemotherapy alone or chemotherapy in combination with immune checkpoint blockade.
Andreas Ullern, Kristian Holm, Nikolai Kragøe Andresen, Andreas Hagen Røssevold, Corinna Bang, Bjørn Naume, Johannes R. Hov, Jon Amund Kyte   +7 more
wiley   +1 more source

Effect of Intragenomic Sequence Heterogeneity among Multiple 16S rRNA Genes on Species Identification of Elizabethkingia

Microbiology Spectrum, 2022
Accurate identification of Elizabethkingia species mostly requires the use of molecular techniques, and 16S rRNA gene sequencing is generally considered the method of choice.
Jiun-Nong Lin, Chung-Hsu Lai, Shang-Yi Lin, Ching-Chi Lee, Nan-Yao Lee, Po-Yu Liu, Chih-Hui Yang, Yi-Han Huang   +7 more
doaj   +1 more source

Microbial profile of the appendix niche in acute appendicitis: a novel sampling approach

FEBS Open Bio, EarlyView.
This study utilized a novel sampling method, ERAT (i.e. endoscopic retrograde appendicitis treatment)‐guided lumen aspiration, to obtain samples from the appendix, and shotgun metagenomic sequencing was performed for in situ characterization of the appendix microbiome in patients with acute appendicitis.
Huimin Ma, Mingfei Wang, Yanhu Feng, Wanqi Zhang, Wenbo Wang, Yanfeng Xie, Guixiang Kong, Jie Feng, Pengfei Wang, Qi Wang, Xiaojun Huang   +10 more
wiley   +1 more source

Erwinia billingiae as Unusual Cause of Septic Arthritis, France, 2017

Emerging Infectious Diseases, 2019
In 2017 in France, we treated a patient with knee septic arthritis caused by Erwinia billingiae after trauma involving a palm tree. This rare pathogen could only be identified through 16S rRNA gene sequencing. For bacterial infections after injuries with
Isabelle Bonnet, Baptiste Bozzi, Eric Fourniols, Stéphane Mitrovic, Olivia Soulier-Escrihuela, Florence Brossier, Wladimir Sougakoff, Jérôme Robert, Stéphane Jauréguiberry, Alexandra Aubry   +9 more
doaj   +1 more source