Results 141 to 150 of about 309,135 (337)

Pre-sequencing assessment of RNA-Seq library quality using real-time qPCR

BioTechniques
RNA sequencing (RNA-Seq) is an essential assay for studying transcriptome profiling. Ribosomal RNA (rRNA) comprises more than 80–90% of total cellular RNA; efficient removal is essential for accurately capturing transcriptomes, particularly to sequence ...
Kavya Kottapalli, Hsu Chao, Qi Jiang, Jimmonique Donelson, Nathanael Emerick, Sravya V Bhamidipati, Zeineen Momin, Tittu Mathew, Ziad M. Khan, Viktoriya Korchina, Marie-Claude Gingras, Donna Muzny, Richard Gibbs, Harsha Doddapaneni   +13 more
doaj   +1 more source

Secondary structure models of 18S and 28S rRNAs of the true bugs based on complete rDNA sequences of Eurydema maracandica Oshanin, 1871 (Heteroptera, Pentatomidae)

ZooKeys, 2013
The sequences of 18S and 28S rDNAs have been used as molecular markers to resolve phylogenetic relationships of Heteroptera for two decades. The complete sequences of 18S rDNAs have been used in many studies, while in most studies only partial sequences ...
Shasha Yu, Yanhui Wang, Dávid Rédei, Qiang Xie, Wenjun Bu   +4 more
doaj   +1 more source

The Protist Ribosomal Reference database (PR2): a catalog of unicellular eukaryote Small Sub-Unit rRNA sequences with curated taxonomy [PDF]

, 2012
The interrogation of genetic markers in environmental meta-barcoding studies is currently seriously hindered by the lack of taxonomically curated reference data sets for the targeted genes.
Probert, Ian, Dolan, John R., Kooistra, Wiebe H.C.F., Guillou, Laure, Vaulot, Daniel, Campo, Javier, Del, Morard, Raphael, Bass, David, Logares, Ramiro, Montresor, Marina, del Campo, Javier, Lara, Enrique, Berney, Cédric, Zimmermann, Pascal, Burgaud, Gaétan, Pawlowski, Jan, Campo, Javier,, Bachar, Dipankar, Boutte, Christophe, Bittner, Lucie, Christen, Richard, Mahe, Frédéric, Dunthorn, Micah, Kooistra, Wiebe H. C. F., ,, Siano, Raffaele, Stoeck, Thorsten, Le Bescot, Noan, Audic, Stéphane, Decelle, Johan, Not, Fabrice, Massana, Ramon, de Vargas, Colomban, Sauvadet, Anne-Laure, Edvardsen, Bente, Holzmann, Maria   +35 more
core   +1 more source

P. falciparum 18S rRNA structure.

, 2022
The canonical structure of the P. falciparum 18S rRNA (from https://crw-site.chemistry.gatech.edu/) was compared with base reactivity data for the assembled sequence of the PF3D7_0725600 gene (encoding blood-stage-expressed 18S rRNA).
Catherine J. Merrick (12117909), Chun Kit Kwok (831586), Franck Dumetz (13570199), Jieyu Zhao (1392445), Anton J. Enright (11331476)   +4 more
core   +1 more source

β‐Elemene Rescues Radiation‐Induced Enteritis by Orchestrating a Host‐Microbiome Circuit That Fuels Epigenetic DNA Repair

Advanced Science, EarlyView.
This study elucidates that β‐elemene promotes cellular uptake of L. gasseri‐derived lactate by enhancing the membrane translocation of MCT1 in a CD147‐dependent manner. Intracellular lactate, through the lactylation of RBBP4 at the K26 site, recruits EP300 to the promoter regions of downstream genes (POLD1/POLD3), catalyzing H3K27ac modification.
Jiancheng He, Jiapeng Bao, Shukang Deng, Weijie Zang, Haoming Yan, Zihao Zhao, Guangze Zhang, Ruiqing Liu, Junjie Chen, Yilin Hu, Wanjiang Xue   +10 more
wiley   +1 more source

PCR-based detection of Plasmodium falciparum in saliva using mitochondrial cox3 and varATS primers

Tropical Medicine and Health, 2018
Background Sampling of saliva for diagnosing Plasmodium falciparum infections is a safe, non-invasive alternative to sampling of blood. However, the use of saliva presents a challenge because lower concentrations of parasite DNA are present in saliva ...
Yukie M. Lloyd, Livo F. Esemu, Jovikka Antallan, Bradley Thomas, Samuel Tassi Yunga, Bekindaka Obase, Nana Christine, Rose G. F. Leke, Richard Culleton, Kenji Obadiah Mfuh, Vivek R. Nerurkar, Diane Wallace Taylor   +11 more
doaj   +1 more source

Groups without cultured representatives dominate eukaryotic picophytoplankton in the oligotrophic South East Pacific Ocean [PDF]

, 2009
Background: Photosynthetic picoeukaryotes (PPE) with a cell size less than 3 µm play a critical role in oceanic primary production. In recent years, the composition of marine picoeukaryote communities has been intensively investigated by molecular ...
Marie, D., Vaulot, Daniel, Jardillier, Ludwig, Marie, Dominique, Xiao Li Shi, Dominique Marie, Shi, Xiao Li, Scanlan, David J., Marie Dominique, Jardillier Ludwig, Scanlan David J., Shi Xiao Li, Vaulot Daniel, David J Scanlan, Daniel Vaulot, Ludwig Jardillier   +15 more
core   +1 more source

UCHL3 Regulates Subgenomic Flaviviral RNA Condensates to Promote Virus Propagation

Advanced Science, EarlyView.
ABSTRACT Flavivirus subgenomic RNAs (sfRNAs) antagonise antiviral defences, yet how sfRNAs are organized and maintained in cells remains poorly understood. Here we identify ubiquitin C‐terminal hydrolase L3 (UCHL3) as a post‐translational regulator of flavivirus sfRNA stability and function.
Oscar Trejo‐Cerro, Anna Beekmayer‐Dhillon, Qi Wen Teo, Lewis Siu, Ming Yuan Li, Kate J. Heesom, Adán Pinto‐Fernández, Sumana Sanyal   +7 more
wiley   +1 more source

Chromosomal localization of the 18S-28S and 5S rRNA genes and (TTAGGG)n sequences of butterfly lizards (Leiolepis belliana belliana and Leiolepis boehmei, Agamidae, Squamata)

Genetics and Molecular Biology, 2011
Chromosomal mapping of the butterfly lizards Leiolepis belliana belliana and L. boehmei was done using the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences. The karyotype of L. b. belliana was 2n = 36, whereas that of L.
Kornsorn Srikulnath, Yoshinobu Uno, Kazumi Matsubara, Amara Thongpan, Saowanee Suputtitada, Somsak Apisitwanich, Chizuko Nishida, Yoichi Matsuda   +7 more
doaj  

Targeting Supramolecular Active Complexes of Nav1.7/Nav1.8 to Relieve Chronic Neuropathic Pain

Advanced Science, EarlyView.
In mice and patients with severe chronic neuropathic pain (NP), Nav1.7, Nav1.8, TrkB, and five cytoskeletal proteins form supramolecular active complexes (SMACs) with polygonal lattice structures as noxious signal amplifiers in dorsal root ganglion (DRG) neurons.
Liting Sun, Hang Xian, Yunxin Shi, Taotan Yang, Hongyan Shuai, Wenchao Hu, Siying Fei, Miao Xu, Taoyuan Yang, Ruilong Xia, Ting Wen, Fengting Zhu, Yan Fu, Yang Li, Wei Xia, Ran Qian, Yuanying Liu, Zhicheng Tian, Lamei Li, Qian Zhou, Lize Xiong, Rui Cong, Ceng Luo, Shengxi Wu, Xiafeng Shen, Xin Yu, Rou‐Gang Xie, Changgeng Peng   +27 more
wiley   +1 more source