Results 21 to 30 of about 122,279 (340)

pr2-primers: an 18S rRNA primer database for protists

open access: yesMolecular Ecology Resources, 2021
AbstractMetabarcoding of microbial eukaryotes (collectively known asprotists) has developed tremendously in the last decade, almost uniquely relying on the 18S rRNA gene. As microbial eukaryotes are extremely diverse, many primers and primer pairs have been developed.
Daniel Vaulot   +3 more
openaire   +6 more sources

Utilization of 18s ribosomal RNA LAMP for detecting Plasmodium falciparum in microscopy and rapid diagnostic test negative patients

open access: yesPLoS ONE, 2022
In this study, Plasmodium falciparum was detected in patients that were declared negative for malaria microscopy and rapid diagnostic test kit (mRDT), using Plasmodium 18s rRNA loop-mediated isothermal amplification (LAMP) technique. The main aim of this
Enoch Aninagyei   +7 more
doaj   +2 more sources

Genetic Diversity of Babesia canis Strains in Dogs in Lithuania

open access: yesMicroorganisms, 2022
Canine babesiosis is an emerging and rapidly expanding tick-borne disease in central and northeast Europe. In the last two decades, the endemic area of Babesia canis has expanded from central Europe to the Baltic region.
Jana Radzijevskaja   +6 more
doaj   +1 more source

Reference Gene Selection for Expression Analyses by qRT-PCR in Dendroctonus valens

open access: yesInsects, 2020
Dendroctonus valens is the main pest of the genus Pinus. To facilitate gene expression analyses, suitable reference genes for adults and mature larvae of D. valens under different temperature conditions were determined.
Chunchun Zheng   +5 more
doaj   +1 more source

Taxonomic resolutions based on 18S rRNA genes: a case study of subclass copepoda. [PDF]

open access: yesPLoS ONE, 2015
Biodiversity studies are commonly conducted using 18S rRNA genes. In this study, we compared the inter-species divergence of variable regions (V1-9) within the copepod 18S rRNA gene, and tested their taxonomic resolutions at different taxonomic levels ...
Shu Wu, Jie Xiong, Yuhe Yu
doaj   +1 more source

Séance: reference-based phylogenetic analysis for 18S rRNA studies [PDF]

open access: yesBMC Evolutionary Biology, 2014
Marker gene studies often use short amplicons spanning one or more hypervariable regions from an rRNA gene to interrogate the community structure of uncultured environmental samples. Target regions are chosen for their discriminatory power, but the limited phylogenetic signal of short high-throughput sequencing reads precludes accurate phylogenetic ...
Medlar Alan   +2 more
openaire   +2 more sources

Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes [PDF]

open access: yesApplied and Environmental Microbiology, 2019
To better understand the diversity of primary producers in the Arctic Ocean, we targeted a nitrogen cycle gene, NR, which is required for phytoplankton to assimilate nitrate into organic forms of nitrogen macromolecules. We compared this to the more detailed taxonomy from ice-influenced stations using a general taxonomic gene (18S rRNA).
André M, Comeau   +4 more
openaire   +2 more sources

Quantification of Plasmodium knowlesi versus Plasmodium falciparum in the rhesus liver: implications for malaria vaccine studies in rhesus models

open access: yesMalaria Journal, 2020
Background Rhesus macaques are valuable pre-clinical models for malaria vaccine development. The Plasmodium knowlesi/rhesus and Plasmodium falciparum/rhesus models are two established platforms for malaria vaccine testing, and both have previously been ...
Melanie J. Shears   +4 more
doaj   +1 more source

Description and molecular phylogeny of a new and one known needle nematode of the genus Paralongidorus (Nematoda: Longidoridae) from grapevine in Portugal [PDF]

open access: yes, 2018
A new and a known longidorid nematode, Paralongidorus lusitanicus n. sp. and Paralongidorus plesioepimikis, are described and illustrated from populations extracted from soil associated with grapevine (Vitis vinifera L.) from Escaroupim and Pó (central ...
Castillo, P   +4 more
core   +1 more source

Home - About - Disclaimer - Privacy