Results 21 to 30 of about 120,971 (346)

Utilization of 18s ribosomal RNA LAMP for detecting Plasmodium falciparum in microscopy and rapid diagnostic test negative patients

PLoS ONE, 2022
In this study, Plasmodium falciparum was detected in patients that were declared negative for malaria microscopy and rapid diagnostic test kit (mRDT), using Plasmodium 18s rRNA loop-mediated isothermal amplification (LAMP) technique. The main aim of this
Enoch Aninagyei, Adjoa Agyemang Boakye, Clement Okraku Tettey, Kofi Adjei Ntiri, Samuel Ohene Ofori, Comfort Dede Tetteh, Thelma Teley Aphour, Tanko Rufai   +7 more
doaj   +2 more sources

Reference Gene Selection for Expression Analyses by qRT-PCR in Dendroctonus valens

Insects, 2020
Dendroctonus valens is the main pest of the genus Pinus. To facilitate gene expression analyses, suitable reference genes for adults and mature larvae of D. valens under different temperature conditions were determined.
Chunchun Zheng, Dongfang Zhao, Yabei Xu, Fengming Shi, Shixiang Zong, Jing Tao   +5 more
doaj   +1 more source

Taxonomic resolutions based on 18S rRNA genes: a case study of subclass copepoda. [PDF]

PLoS ONE, 2015
Biodiversity studies are commonly conducted using 18S rRNA genes. In this study, we compared the inter-species divergence of variable regions (V1-9) within the copepod 18S rRNA gene, and tested their taxonomic resolutions at different taxonomic levels ...
Shu Wu, Jie Xiong, Yuhe Yu
doaj   +1 more source

Séance: reference-based phylogenetic analysis for 18S rRNA studies [PDF]

BMC Evolutionary Biology, 2014
Marker gene studies often use short amplicons spanning one or more hypervariable regions from an rRNA gene to interrogate the community structure of uncultured environmental samples. Target regions are chosen for their discriminatory power, but the limited phylogenetic signal of short high-throughput sequencing reads precludes accurate phylogenetic ...
Medlar Alan, Löytynoja Ari, Aivelo Tuomas   +2 more
openaire   +2 more sources

Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes [PDF]

Applied and Environmental Microbiology, 2019
To better understand the diversity of primary producers in the Arctic Ocean, we targeted a nitrogen cycle gene, NR, which is required for phytoplankton to assimilate nitrate into organic forms of nitrogen macromolecules. We compared this to the more detailed taxonomy from ice-influenced stations using a general taxonomic gene (18S rRNA).
André M, Comeau, Marcos G, Lagunas, Karen, Scarcella, Diana E, Varela, Connie, Lovejoy   +4 more
openaire   +2 more sources

Quantification of Plasmodium knowlesi versus Plasmodium falciparum in the rhesus liver: implications for malaria vaccine studies in rhesus models

Malaria Journal, 2020
Background Rhesus macaques are valuable pre-clinical models for malaria vaccine development. The Plasmodium knowlesi/rhesus and Plasmodium falciparum/rhesus models are two established platforms for malaria vaccine testing, and both have previously been ...
Melanie J. Shears, Annette M. Seilie, B. Kim Lee Sim, Stephen L. Hoffman, Sean C. Murphy   +4 more
doaj   +1 more source

Free-living protozoa in two unchlorinated drinking water supplies identified by phylogenic analysis of 18S rRNA gene sequences [PDF]

, 2009
Free-living protozoan communities in water supplies may include hosts for Legionella pneumophila and other undesired bacteria and also pathogens. This study aimed at identifying free-living protozoa in two unchlorinated groundwater supplies using ...
Valster, R.M., Wullings, B.A., Bakker, G., Smidt, H., Kooij, D., van der   +4 more
core   +1 more source

Sequential Ubiquitination of Ribosomal Protein uS3 Triggers the Degradation of Non-functional 18S rRNA

Cell Reports, 2019
Summary: 18S non-functional rRNA decay (NRD) eliminates non-functional 18S rRNA with deleterious mutations in the decoding center. Dissociation of the non-functional 80S ribosome into 40S and 60S subunits is a prerequisite step for degradation of the non-
Takato Sugiyama, Sihan Li, Misaki Kato, Ken Ikeuchi, Atsushi Ichimura, Yoshitaka Matsuo, Toshifumi Inada   +6 more
doaj   +1 more source

Quantitative analysis of snoRNA association with pre-ribosomes and release of snR30 by Rok1 helicase [PDF]

, 2008
In yeast, three small nucleolar RNAs (snoRNAs) are essential for the processing of pre-ribosomal RNA—U3, U14 and snR30—whereas 72 non-essential snoRNAs direct site-specific modification of pre-rRNA. We applied a quantitative screen for alterations in the
Bohnsack, Markus, Kos, Martin, Tollervey, David   +2 more
core   +1 more source

Plasmodium 18S rRNA of intravenously administered sporozoites does not persist in peripheral blood

Malaria Journal, 2018
Background Plasmodium 18S rRNA is a biomarker used to monitor blood-stage infections in malaria clinical trials. Plasmodium sporozoites also express this biomarker, and there is conflicting evidence about how long sporozoite-derived 18S rRNA persists in ...
Sean C. Murphy, Andrew S. Ishizuka, Zachary P. Billman, Tayla M. Olsen, Annette M. Seilie, Ming Chang, Nahum Smith, Vorada Chuenchob, Sumana Chakravarty, B. Kim Lee Sim, Stefan H. I. Kappe, Stephen L. Hoffman, Robert A. Seder   +12 more
doaj   +1 more source