Results 1 to 10 of about 285 (89)

Species-resolved sequencing of low-biomass or degraded microbiomes using 2bRAD-M [PDF]

Genome Biology, 2022
Microbiome samples with low microbial biomass or severe DNA degradation remain challenging for amplicon-based or whole-metagenome sequencing approaches. Here, we introduce 2bRAD-M, a highly reduced and cost-effective strategy which only sequences ~ 1% of
Meng Zhang, Gongchao Jing
exaly   +3 more sources

Identification of Two Clusters in Renal Pelvis Urobiome of Unilateral Stone Formers Using 2bRAD-M [PDF]

Microorganisms, 2023
Urolithiasis is a common urological disease with increasing incidence and a high recurrence rate, whose etiology is not fully understood. The application of sequencing and culturomics has revealed that urolithiasis is closely related to the urinary ...
Sen-Yuan Hong, Lintao Miao
exaly   +4 more sources

The renal pelvis urobiome in the unilateral kidney stone patients revealed by 2bRAD-M [PDF]

Journal of Translational Medicine, 2022
Background The pathogenesis of kidney stone disease (KSD) is not fully understood, and potential contributing factors remain to be explored. Several studies have revealed that the urinary microbiome (urobiome) of stone formers was distinct from that of ...
Qidong Xia, Shaogang Wang, Yang Xun
exaly   +3 more sources

Unveiling early-life microbial colonization profile through characterizing low-biomass maternal-infant microbiomes by 2bRAD-M [PDF]

Frontiers in Microbiology
IntroductionThe microbial composition of human breast milk and infant meconium offers critical insights into the early microbial colonization profile, and it greatly contributes to the infant’s immune system and long-term health outcomes.
Tianfan Cheng, Lijian Jin, Shi Huang
exaly   +4 more sources

High-resolution microbiome analysis of host-rich samples using 2bRAD-M without host depletion [PDF]

Npj Biofilms and Microbiomes
Characterizing human microbiota in host-dominated samples is crucial for understanding host-microbe interactions, yet is challenged by the high host DNA context (HoC). Current depletion strategies are limited by DNA loss and require immediate processing.
Shi Huang
exaly   +3 more sources

Urobiome of patients with diabetic kidney disease in different stages is revealed by 2bRAD-M [PDF]

Journal of Translational Medicine
Background Knowledge of the urinary microbiome (urobiome) in diabetic kidney disease (DKD) remains limited. The most commonly used 16S rRNA sequencing technique can only provide bacterial identification at the genus level.
Hailing Zhao
exaly   +3 more sources

Vaginal microbiota in term pregnant women with differences in cervical ripeness revealed by 2bRAD-M [PDF]

BMC Microbiology
Background Cervical ripening is a multifactorial outcome, and the association between cervical ripening and vaginal microbiota remains unexplored in term primiparous women.
Zongzhi Yin
exaly   +3 more sources

2bRAD-M reveals differences in microbial communities between Modic changes and disc herniation [PDF]

Frontiers in Cellular and Infection Microbiology
BackgroundModic changes are caused by various factors, such as degenerative processes, inflammation, biomechanical, genetic, and metabolic factors, infection, and smoking. Bacteria have been identified in human intervertebral discs by 16S rRNA sequencing;
Fang Yang, Jingtao Zhang, Junpu Zha, Guolei Zhang, Jia Li, Wei Du, Lin Liu, Jun Di   +7 more
exaly   +4 more sources

Microbial succession in human tissues postmortem: insights from 2bRAD-M sequencing [PDF]

Microbiology Spectrum
Microbial communities play a crucial role in decomposition, yet their patterns in human tissues remain underexplored. Most previous research has often focused on animal models such as mice and swine, with limited studies on human samples, primarily ...
Chengtao Li, Shilin Li, Suhua Zhang
exaly   +4 more sources

2bRAD-M Reveals the Characteristics of Urinary Microbiota in Overweight Patients with Urinary Tract Stones [PDF]

Biomedicines
Background: Urinary tract stone (UTS) is a common disease significantly impacting human health. Obesity influences stone formation and increases UTS incidence, yet the differences in the urinary microbiota and pathways between overweight and healthy ...
Xudong Yao, Wentao Zhang, Pengfei Wu
exaly   +4 more sources