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Efficient design and production of glycosylated PEDV spike S1 protein. [PDF]

Sci Rep
Uhlemann EM, Patel R, Thivierge B, Hamonic G, Köster W, Racine T, Liu Q.   +6 more
europepmc   +1 more source

2D-PAGE/2D-DIGEの基本・新技術と応用

Abstracts for Annual Meeting of Japanese Proteomics Society, 2011
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Proteome Analysis with Classical 2D-PAGE

2021
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is based on the combination of two orthogonal separation techniques. In the first dimension, proteins are separated by their isoelectric point, a technique known as isoelectric focusing (IEF).
Caroline, May, Frederic, Brosseron, Kathy, Pfeiffer, Kristin, Fuchs, Helmut E, Meyer, Barbara, Sitek, Katrin, Marcus   +6 more
openaire   +2 more sources

Multi-Image 2D-PAGE Feature Detection

Digital Image Computing: Techniques and Applications (DICTA'05), 2005
Although there have been many reports on computerised methods of spot detection for 2D-PAGE gel images, these methods examine one gel image at a time. Different spot detection methods are negatively affected by the multitude of different blemishes that may occur in gel images in different ways.
Nick Seow, Arcot Sowmya, Changming Sun
openaire   +2 more sources

2D-PAGE Maps Analysis

2008
Due to the low reproducibility affecting 2D gel-electrophoresis and the complex maps provided by this technique, the use of effective and robust methods for the comparison and classification of 2D maps is a fundamental tool for the development of automated diagnostic methods. A review of classical and recently developed methods for the comparison of 2D
MARENGO, Emilio, ROBOTTI, Elisa, Bobba Marco   +2 more
openaire   +3 more sources

Proteome Analysis with Classical 2D-PAGE

2012
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is based on the combination of two orthogonal separation techniques. In the first dimension, proteins are separated by their isoelectric point, a technique known as isoelectric focusing (IEF).
Caroline, May, Frederic, Brosseron, Kathy, Pfeiffer, Helmut E, Meyer, Katrin, Marcus   +4 more
openaire   +2 more sources

Koordinatenbestimmung von Proteinpunkten in 2D-PAGE-Gelen

BIOspektrum, 2014
A major problem in two-dimensional protein gel electrophoresis is the little comparability of gel images. A reference grid of marker proteins in parallel to the separation of the analyte by use of two fluorescent dyes allows the correction of protein coordinates. The deviation from mean is improved by an order of magnitude.
Marina Hanneken, Doreen Ackermann, Simone König, Günter Thesseling   +3 more
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Determination of Posttranslational Modifications by 2D PAGE: Applications to Polyamines

2017
Polyamines not only affect transcription and translation but also may induce a number of posttranslational modifications. The identification of polyamine-induced posttranslational modifications can be performed by 2D PAGE analyses. Here, we provide a protocol for 2D-gel electrophoresis that has been optimized for plants.
Marta, Bitrián, Antonio F, Tiburcio, Rubén, Alcázar   +2 more
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Mass spectrometric characterization of stathmin isoforms separated by 2D PAGE

Journal of Mass Spectrometry, 1999
In proteome analysis, the determination of the phosphorylation status of proteins and protein isoforms, which have been separated by two-dimensional polyacrylamide gel electrophoresis (2D PAGE), is of prime importance in addition to their identification.
D R, Müller, P, Schindler, M, Coulot, H, Voshol, J, van Oostrum   +4 more
openaire   +2 more sources