Results 251 to 260 of about 5,167,436 (292)
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Comparison of MS/MS Methods for Protein Identification from 2D-PAGE
Journal of Proteome Research, 2006We have compared the use of a low resolution MALDI-Ion Trap MS/MS and a high-resolution ESI-TOF-MS/MS for the analysis of spots from 2D gels. The main criteria were speed and accuracy of protein identification. The results obtained using the MALDI-MS/MS system are comparable to those from the LC-MS/MS system in terms of accuracy, but less low-level ...
ARRIGONI, GIORGIO +4 more
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2D-PAGE Analysis Using Evolutionary Computation
2009This paper presents the preliminary studies for the creation of a new tool to assist in medical diagnostic. The tool will help in the analysis of 2D-PAGE images. In order to create a 2D-PAGE image of an ideal patient—the patient could be healthy or ill—the tool will help us in the creation of an image that facilitates and speeds up future diagnostics ...
Pablo Mesejo +3 more
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Purification and Analysis of Variant and Modified Histones Using 2D PAGE
2008Two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE) systems employing combinations of acetic acid/urea (AU), acetic acid/urea/Triton X-100 (AUT) and sodium dodecyl sulfate (SDS) gel formulations are uniquely effective for resolution of histone variants and their modified derivatives.
George R, Green, Duc P, Do
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2D and 3D Equalizers for Page-Oriented Optical Memories
Optics in Computing, 1997In this study we investigate the use of 2D and 3D equalizers to suppress intersymbol interference (ISI) in 2D parallel channels. The techniques demonstrated here are best suited for parallel implementation and can be used for any page-oriented readout application including increasing the data density achievable in volume optical memories or increasing ...
Mark M. Wang +2 more
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Automated PreScan Function for Scanning Fluorescently Stained 2D-PAGE Gels
Journal of Proteome Research, 2005To automate the acquisition of images from fluorescently stained gels, the power of the excitation laser(s) must be optimized for each sample to prevent spot saturation (or to allow unimportant spots to saturate) yet still retaining sensitivity. In this work, we describe the implementation and effectiveness of a pre-scan function in a robotic solution ...
Peter, Bäck +2 more
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How to Analyze Protein Complexes by 2D Blue Native SDS-PAGE
PROTEOMICS, 2007Natural compartmentalization makes proteome analysis of the cell, cell organelles and organelle subfractions possible. Protein complexes are the basis for the next level of compartmentalization that can be addressed well with proteomic technology. Protein complexes organize and maintain the cellular and organelle functions on all levels of complexity ...
Veronika, Reisinger +1 more
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Sequential Analysis of N- and O-Linked Glycosylation of 2D-PAGE Separated Glycoproteins
Journal of Proteome Research, 2002A robust method has been developed that allows analysis of both N- and O-linked oligosaccharides released from glycoproteins separated using 2D-PAGE and then electroblotted to PVDF membrane. This analysis provides efficient oligosaccharide profiling applicable to glycoproteomic analysis.
Wilson, Nicole L. +3 more
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2D PAGE Databases for Proteins in Human Body Fluids
2008With the development of the Internet, a growing number of proteomics databases have become available. The web is a powerful tool for data integration because it links the components constituting these databases, in general gel images and protein information, while offering rapid means to navigate from one database to another.
Christine Hoogland +4 more
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Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE
2018Absolute protein quantification for the analysis of proteome dynamics is more and more required by the scientific community. Therefore a number of methods have recently been reported that aim at determining concentrations of single proteins in complex samples, all of them having their advantages and limitations.
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