Results 61 to 70 of about 5,167,436 (292)
2D-DIGE analysis of the Xoo-induced secretome in O.meyeriana suspension cultured medium.
, 2016 Secreted-proteins were extracted from the medium and separated by 2D-DIGE using pH 4-7/24cm linear IPG strips and 12.5% SDS-PAGE gels. 110 protein spots showed significant changes (> 1.5 fold; p< 0.05), corresponding to 34 proteins identified by MS.
XianPing Fang (2794246) +6 more
core +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
Journal of Crop Protection, 2016 This study was undertaken to find out the optimum physicochemical parameters of fermentation, i.e. pH, incubation temperature and incubation time for the cellulase enzyme production of Trichoderma harzianum. The extracellular protein content was estimated
Samira Shahbazi +2 more
doaj
Frontiers in Plant Science, 2016 To survive under highly variable environmental conditions, higher plants have acquired a large variety of acclimation responses. Different strategies are used to cope with changes in light intensity with the common goal of modulating the functional ...
Ludwik W Bielczynski +2 more
doaj +1 more source
FEBS Letters, EarlyView.Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil +3 more
wiley +1 more source
A simple construction method for sequentially tidying up 2D online freehand sketches [PDF]
, 2006 This paper presents a novel constructive approach to sequentially tidying up 2D online freehand sketches for further 3D interpretation in a conceptual design system.
Qin, S F, Wright, D, Jordanov, I
core +4 more sources
Effects of Different Methods of Slaughtering on Protein Expression in Chicken Meat
International Islamic University Malaysia Engineering Journal, 2012 : This study investigated the variation in total protein profile in chicken skeletal muscle which is thought to be influenced by variations in the method of slaughtering.
Rahela Zaman +4 more
doaj +1 more source
Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining
Molecular Oncology, EarlyView.IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis +3 more
wiley +1 more source
What is Normalization? The Strategies Employed in Top-Down and Bottom-Up Proteome Analysis Workflows
Proteomes, 2019 The accurate quantification of changes in the abundance of proteins is one of the main applications of proteomics. The maintenance of accuracy can be affected by bias and error that can occur at many points in the experimental process, and normalization ...
Matthew B. O’Rourke +7 more
doaj +1 more source
Molecular Oncology, EarlyView.We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober +16 more
wiley +1 more source