Results 251 to 260 of about 10,293,161 (301)
Vascularization in 3D Cell Culture
, 2021 The two-dimensional (2D) monoculture does not recapitulate the three-dimensional (3D) environment of in vivo vasculature. Endothelial cell (ECs) spheroids have been introduced as a 3D in vitro model exhibiting angiogenic responses and sprouting behavior in vivo.
M. Markou +4 more
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Electromolding for 3D cell culture
10th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, 2015Constructing microstructures with crosslinkable biomaterials, gelatin methacrylate (GelMA) and poly(ethylene glycol) diacrylate (PEGDA), containing fibroblasts (NIH-3T3) are demonstrated using electromolding that combines electrowetting and dielectrophoresis on an electromicrofluidic platform without physical molds.
Yi-Han Lai, Shih-Kang Fan
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Introduction to 3D Cell Culture
2021This chapter explains the importance of 3D cell culture and highlights its potential and benefits in comparison to traditional 2D cultivation. Since 3D cell culture is supposed to mimic the in vivo situation, we will also have a close look on the complex composition of the in vivo microenvironment and why 2D cultivation does not resemble the in vivo ...
Dominik Egger, Sabrina Nebel
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2021
One of the fast growing 3D mammalian cell culture platforms are hydrogels—three-dimensional, crosslinked networks of polymers. In this chapter we describe the fundamentals of hydrogels and provide an overview of sources from which hydrogels can be derived, such as animal, non-animal, synthetic, or combinations.
Antonina Lavrentieva, Jane Spencer-Fry
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One of the fast growing 3D mammalian cell culture platforms are hydrogels—three-dimensional, crosslinked networks of polymers. In this chapter we describe the fundamentals of hydrogels and provide an overview of sources from which hydrogels can be derived, such as animal, non-animal, synthetic, or combinations.
Antonina Lavrentieva, Jane Spencer-Fry
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A 3D cell culture approach for studying neuroinflammation
Journal of Neuroscience Methods, 2021Neurodegenerative diseases are highly complex making them challenging to model in cell culture. All cell types of the brain have been implicated as exerting an effect on pathogenesis, and disease progression is likely influenced by the cross-talk between the different cell types.
James A. Carroll +2 more
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3D Cell Culture: An Introduction
20173D cell culture is an invaluable tool in developmental, cell, and cancer biology. By mimicking crucial features of in vivo environment, including cell-cell and cell-extracellular matrix interactions, 3D cell culture enables proper structural architecture and differentiated function of normal tissues or tumors in vitro.
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Viscoelastic hydrogels for 3D cell culture
Biomaterials Science, 2017This mini-review discusses newly developed approaches to tuning hydrogel viscoelasticity and recent studies demonstrating an impact of viscoelasticity on cells.
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2020
In this chapter, we go over the main principles and protocols that enable to keep cells and tissues alive in an in vitro environment. This process is called “cell culturing” and can be used for both isolated cells and small tissue constructs. After touching upon main terms such as primary, secondary, continuous, and finite cell lines, we focus on ...
Astghik Karapetyan, Narine Sarvazyan
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In this chapter, we go over the main principles and protocols that enable to keep cells and tissues alive in an in vitro environment. This process is called “cell culturing” and can be used for both isolated cells and small tissue constructs. After touching upon main terms such as primary, secondary, continuous, and finite cell lines, we focus on ...
Astghik Karapetyan, Narine Sarvazyan
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Esophageal stem cells and 3D‐cell culture models
Annals of the New York Academy of Sciences, 2011The following on esophageal stem cells and 3D‐cell culture models contains commentaries on metaplasia through transdifferentiation and through stem cells; transcription factors that may determine an intestinal‐like phenotype; the in vitro, organotypic cell culture models; and the role of stem cells in Barrett's esophagus and its dysplastic progression.
Rhonda F, Souza +2 more
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