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3D Cell Culture Models

2017
In the 1960s, scientists searching for a cell-based system that would reflect the physiology and heterogeneity of solid tumors in a well-controlled experimental setting developed multicell spheroid cultures. Early studies focused on the biology, physiology, and drug and radiation response of spheroids.
David M. Evans, Beverly A. Teicher
openaire   +1 more source

3D Cell Culture Systems: Advantages and Applications

Journal of Cellular Physiology, 2014
Cell cultures are important material of study for the variety of advantages that they offer. Both established continuous cell lines and primary cell cultures continue to be invaluable for basic research and for direct applications. Technological advancements are necessary to address emerging complex challenges and the way cells are cultured in vitro is
Maddaly, Ravi   +4 more
openaire   +2 more sources

Injectable thermogel for 3D culture of stem cells

Biomaterials, 2018
Thermogel is an aqueous polymer solution that undergoes sol-to-gel transition as the temperature increases. Cells, growth factors, and signaling molecules can be incorporated simultaneously during the sol-to-gel transition. The cytocompatible procedure makes the thermogel an excellent platform for 3D culture of stem cells.
Madhumita, Patel   +4 more
openaire   +2 more sources

Nanoimprinting of Topographical and 3D Cell Culture Scaffolds

Nanomedicine, 2014
The extracellular matrix exhibits several nanostructures such as fibers, filaments, nanopores and ridges that can be mimicked by topographical and 3D substrates for cell and tissue cultures for an environment closer to in vivo conditions. This review summarizes and discusses a growing number of reports employing nanoimprint lithography to obtain such ...
Maha, Elsayed, Olivia M, Merkel
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Contributions of 3D Cell Cultures for Cancer Research

Journal of Cellular Physiology, 2017
Cancer cell lines have contributed immensely in understanding the complex physiology of cancers. They are excellent material for studies as they offer homogenous samples without individual variations and can be utilised with ease and flexibility.
Maddaly, Ravi   +2 more
openaire   +2 more sources

3D-FISH on Cultured Cells Combined with Immunostaining

2010
Fluorescence in situ hybridization on three-dimensionally preserved nuclei (3D-FISH), in combination with immunocytochemistry and 3D fluorescence microscopy, is a key tool to analyze the functional organization of the interphase nucleus. In the last decade, 3D-FISH on cultured cells has become a routine technique and is now widely used in nuclear ...
Irina, Solovei, Marion, Cremer
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3D Cell Culture

2018
Ranjna C. Dutta, Aroop K. Dutta
openaire   +1 more source

Cell culture vessel for 3D culture and methods of culturing 3D cells

2023
FERRIE ANN MEEJIN   +2 more
openaire   +1 more source

3D Cell Culture

Methods in Molecular Biology, 2017
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