Novel microRNAs modulating ecto-5′-nucleotidase expression [PDF]
Frontiers in Immunology, 2023 IntroductionThe expression of immune checkpoint molecules (ICMs) by cancer cells is known to counteract tumor-reactive immune responses, thereby promoting tumor immune escape.
Theresa Kordaß +4 more
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A rare mutation (p.F149del) of the NT5C3A gene is associated with pyrimidine 5′-nucleotidase deficiency [PDF]
Cellular & Molecular Biology Letters, 2022 Pyrimidine 5′-nucleotidase deficiency is a rare erythrocyte enzymopathy. Here we report two cases of hemolytic anemia in brothers of Polish origin that are associated with a very rare mutation.
Dżamila M. Bogusławska +6 more
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Ifn1 is an intracellular GMP 5′-nucleotidase induced during the fission yeast response to phosphate starvation [PDF]
mBioSchizosaccharomyces pombe adapts to phosphate starvation by upregulating the expression of (i) a cell-surface acid phosphatase, Pho1, that mobilizes inorganic phosphate from the extracellular milieu; (ii) transmembrane transporters that take up inorganic
Aleksei Innokentev +2 more
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Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines [PDF]
Cells, 2021 Enzymes of nucleoside and nucleotide metabolism regulate important cellular processes with potential impacts on nucleotide-unrelated parameters. We have used a set of CRISPR/Cas9-modified cell models expressing both, one, or none of the 5′-nucleotidases ...
Octavia Cadassou +9 more
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Targeted deletion of ecto-5′-nucleotidase results in retention of inosine monophosphate content in postmortem muscle of medaka (Oryzias latipes) [PDF]
Scientific Reports, 2022 Inosine monophosphate (IMP) is an important indicator of meat freshness and contributes to its umami taste. An attractive strategy for enhancing umami is to suppress the IMP-degrading activity and increase the IMP content in the skeletal muscle through ...
Yu Murakami +6 more
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New Method for 5′−Nucleotidase Preparation and Evaluation of Its Catalytic Activity [PDF]
FoodsIn this study, we established a new methodology for preparing 5′−nucleotidase (5′−NT) with the aim of enhancing our understanding of its enzyme activity and laying a basis for regulating the content of umami−enhancing nucleotides in pork.
Yin Zhang +7 more
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Biochemical Characterization of an Arabinoside Monophosphate Specific 5′-Nucleotidase-like Enzyme from Streptomyces antibioticus [PDF]
BiomoleculesTo investigate the function of the gene penF in the pentostatin and vidarabine (Ara-A) biosynthetic gene cluster in Streptomyces antibioticus NRRL 3238, PenF was recombinantly expressed and characterized.
Yuxue Liu +6 more
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5′-Nucleotidase Plays a Key Role in Uric Acid Metabolism of Bombyx mori [PDF]
Cells, 2021 Uric acid (UA) is the end-product in the human purine metabolism pathway. The UA that accumulates in silkworm tissues is excreted as a nitrogen waste product.
Linmeng Tang +11 more
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Expression and Site-Specific Biotinylation of Human Cytosolic 5′-Nucleotidase 1A in Escherichia coli [PDF]
Methods and ProtocolsAutoantibodies targeting cytosolic 5′-nucleotidase 1A (cN1A) are found in several autoimmune diseases, including inclusion body myositis (IBM), Sjögren’s syndrome, and systemic lupus erythematosus.
Nataliya Slater +6 more
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Ecto-5′-Nucleotidase (CD73) Regulates the Survival of CD8+ T Cells [PDF]
Frontiers in Cell and Developmental Biology, 2021 Ecto-5′-nucleotidase (CD73) is an enzyme present on the surface of tumor cells whose primary described function is the production of extracellular adenosine. Due to the immunosuppressive properties of adenosine, CD73 is being investigated as a target for
Mariana V. Rosemblatt +16 more
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