Results 181 to 190 of about 30,373 (230)

Fluorometric measurement of 5-aminolevulinic acid in serum

Clinica Chimica Acta, 2004
Measurement of 5-aminolevulinic acid (ALA) in serum is potentially useful in acute porphyrias, lead poisoning and hereditary tyrosinemia. Because levels of ALA in serum are about 100 times less than in urine, a highly sensitive method is required. We describe a simple and sensitive fluorometric method that does not require HPLC.ALA is separated from ...
Chul, Lee, Xian, Qiao, Douglas E, Goeger, Karl E, Anderson   +3 more
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Biosynthesis of 5-Aminolevulinic Acid

2009
5-Aminolevulinic acid (ALA) is the general precursor of all known tetrapyrroles. Currently, two different biosynthetic routes for ALA formation are known. Humans, animals, fungi and the α-group of the proteobacteria employ the one-step-condensation of succinyl-coenzyme A and glycine catalyzed by pyridoxal 5′-phosphate-dependent ALA synthase. In plants,
Dieter Jahn, Dirk W. Heinz
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5-Aminolevulinic acid synthase: mechanism, mutations and medicine

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2003
5-Aminolevulinic acid synthase (ALAS), the first enzyme of the heme biosynthesis pathway, catalyses the pyridoxal 5'-phosphate-dependent condensation between glycine and succinyl-CoA to yield 5-aminolevulinic acid (5-amino-4-oxopentanoate). A three-dimensional structural model of Rhodobacter spheroides ALAS has been constructed and used to identify ...
Shoolingin-Jordan, P.M., Al-Daihan, S., Alexeev, D., Baxter, R.L., Bottomley, S.S., Kahari, I.D., Roy, I., Sarwar, M., Sawyer, L., Wang, S.F.   +9 more
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Microbial production and applications of 5-aminolevulinic acid

Applied Microbiology and Biotechnology, 2014
5-Aminolevulinic acid (ALA), an important intermediate in tetrapyrrole biosynthesis in organisms, has been widely applied in many fields, such as medicine, agriculture, and the food industry, due to its biochemical characteristics. Research efforts supporting the microbial production of ALA have received increasing interest due to its dominant ...
Shuli, Liu, Guangming, Zhang, Xiangkun, Li, Jie, Zhang   +3 more
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5-Aminolevulinic Acid Induces Iron Release from Ferritin

Archives of Biochemistry and Biophysics, 1995
5-Aminolevulinic acid (ALA), a heme precursor accumulated in acute intermittent porphyria (AIP) and lead poisoning, undergoes metal-catalyzed oxidation in air-equilibrated solutions buffered at neutral pH, yielding free radicals (O2, HO. and ALA.).
P I, Oteiza, C G, Kleinman, M, Demasi, E J, Bechara   +3 more
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The stability of 5-aminolevulinic acid in solution

Journal of Photochemistry and Photobiology B: Biology, 2002
5-Aminolevulinic acid (ALA) is being assessed for photodynamic therapy of cancer and other diseases worldwide. However, its stability properties in solution are not well understood yet. The breakdown of ALA in pH-buffered solutions was examined in this work.
Øystein Bech, Gadmar, Johan, Moan, Eldri, Scheie, Li-Wei, Ma, Qian, Peng   +4 more
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Intraoperative 5-aminolevulinic-acid-induced fluorescence in meningiomas

Acta Neurochirurgica, 2010
5-aminolevulinic acid (5-ALA) has gained importance as an intraoperative photodynamic diagnostic agent for the extirpation of malignant gliomas. The application of this technique for resection of meningiomas has barely been explored. The aim of this study was to evaluate the utility of 5-ALA-induced fluorescence as a visual tool in meningioma resection
Daniel, Coluccia, Javier, Fandino, Masayuki, Fujioka, Susanne, Cordovi, Carl, Muroi, Hans, Landolt   +5 more
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Fluorescence diagnosis of endometriosis using 5-aminolevulinic acid

Surgical Endoscopy, 2000
The diagnosis of nonpigmented endometrial lesions by simple laparoscopic visualization is difficult and often inaccurate. We therefore sought to establish a new and more accurate method to visualize these nonpigmented peritoneal changes caused by endometriosis.A total of 37 patients received 30 mg 5-aminolevulinic acid/kg body weight 10 to 14 hs prior ...
E, Malik, C, Berg, A, Meyhöfer-Malik, O, Buchweitz, P, Moubayed, K, Diedrich   +5 more
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Degradation mechanism and stability of 5‐aminolevulinic acid

Journal of Pharmaceutical Sciences, 2000
The physiological substance and precursor of the heme synthesis 5-aminolevulinic acid (ALA) is a promising prodrug for photodiagnosis and photodynamic therapy of epithelial tumors, particularly in urological and gynecological tissues. For the clinical use of this substance, a chemically stable and sterile drug formulation is required.
A, Bunke, O, Zerbe, H, Schmid, G, Burmeister, H P, Merkle, B, Gander   +5 more
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5-Aminolevulinic Acid: Acne Vulgaris

2011
Photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) is a widely used, although off-label, treatment for moderate to severe acne in the USA. Still in its infancy, this treatment has confounded our better wisdom for best practices as studies have been limited and largely carried out in small, investigator-initiated clinical trials.
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