Results 71 to 80 of about 32,863 (259)

Epidermal METTL1‐Mediated m7G Modification Drives Psoriatic Inflammation by Stabilizing Bdkrb1 and Orchestrating Neutrophil Recruitment

Advanced Science, EarlyView.
This study unveils an unrecognized pro‐inflammatory epitranscriptomic checkpoint in psoriasis. By installing m7G modifications on the 5′ UTR of Bdkrb1 mRNA, METTL1 enhances receptor stability to orchestrate keratinocyte‐driven neutrophil recruitment via p38 MAPK signaling.
Chang Zhang, Jiayi Lu, Sihao Yan, Yirui Wang, Zhuo Li, Weiwei Chen, Yang Han, Ziyan Zhang, Yafen Yu, Qi Zhen, Liangdan Sun   +10 more
wiley   +1 more source

Common AAV gene therapy vectors show nonselective transduction of ex vivo human brain tissue

Molecular Therapy: Methods & Clinical Development
The ability to deliver a therapeutic sequence to a specific cell type in the human brain would make possible innumerable therapeutic options for some of our most challenging diseases; however, studies on adeno-associated virus (AAV) vector tropism have ...
JP McGinnis, Joshua Ortiz-Guzman, Maria Camila Guevara, Sai Mallannagari, Benjamin D.W. Belfort, Suyang Bao, Snigdha Srivastava, Maria Morkas, Emily Ji, Angela Addison, Evelyne K. Tantry, Sarah Chen, Ying Wang, Zihong Chen, Kalman A. Katlowitz, Jeffrey J. Lange, Melissa M. Blessing, Carrie A. Mohila, M. Cecilia Ljungberg, Guillermo Aldave, Ali Jalali, Akash Patel, Sameer A. Sheth, Howard L. Weiner, Shankar Gopinath, Ganesh Rao, Akdes Serin Harmanci, Daniel J. Curry, Benjamin R. Arenkiel   +28 more
doaj   +1 more source

Targeting Dnmt3a/m5C/RelA Axis Attenuates Microglia Inflammatory Response and Improves Postoperative Recovery in Chronic Compressive Cervical Spinal Cord Injury

Advanced Science, EarlyView.
A pro‐inflammatory microglial subset persists after decompression in chronic compressive cervical spinal cord injury and shows enhanced Dnmt3a‐driven m5C signaling. By stabilizing RelA mRNA, this axis sustains NF‐κB activation and postoperative neuroinflammation.
Tianyu Qin, Yuan Jiang, Yongheng Xie, Huanwei Qu, Wenbin Yan, Jingle Chen, Yining Chen, Huayi Li, Naibo Feng, Jiajun Wu, Chao Zhang, Zhengqi Huang, Ming Shi, Zhihuai Deng, Guozhi Xiao, Houqing Long   +15 more
wiley   +1 more source

mGem: AAV, from almost a virus to an awesome vector—or is it?

mBio
Adeno-associated virus (AAV) vectors have taken center stage for gene therapy and have shown clinical efficacy in 15 human diseases to date. The Food and Drug Administration has approved seven AAV “drugs” for one-time treatment respectively for Leber’s ...
Arun Srivastava
doaj   +1 more source

PRMT9 Aggravated Dopaminergic Neurodegeneration in Parkinson's Disease Model by Facilitating the Degradation of DUSP26 and Inducing Mitochondrial Dysfunction

Advanced Science, EarlyView.
In the pathological state of PD induced by MPP+, the upregulated PRMT9 in dopaminergic neurons translocates into mitochondrion and interacts with DUSP26 and catalyzes its arginine methylation, leading to the ubiquitin‐proteasomal degradation of DUSP26 mediated by Trim32.
Tengfei Liu, Xiaomeng Song, Xin Guo, Rui Xiao, Ru Sun, Qiuran Ji, Lu Yu, Yiquan Li, Qingyi Fu, Qidi Xue, Lingjun Kong, Lin Chen, Chengjiang Gao, Huiqing Liu   +13 more
wiley   +1 more source

Synergistic HMGN1 and VP64 Fusions Potentiate High‐Precision and PAM‐Flexible Base Editing

Advanced Science, EarlyView.
A novel CDA1Δ‐SpRY architecture fused with HMGN1 and VP64 yields a nearly PAM‐less base editing platform. By focusing cytosine conversion predominantly at position −18, this synergistic complex ensures highly precise targeting. Demonstrating enhanced efficiency across diverse models, including yeast and rice, the platform offers a robust solution for ...
Xi Luo, Yuyao Qu, Zhengyan Ye, Zheng Li, Yuanyan Zhang, Longjiong Luo, Shaokang Li, Wei Zhao, Ming Wang, Ralph Bock, Jianmin Wan, Junjie Tan   +11 more
wiley   +1 more source

ZNF33B Promotes Japanese Encephalitis Virus Infection by Regulating the Stability of M6A‐Modified Trim25 to Control the Autophagy Process

Advanced Science, EarlyView.
Upon JEV infection, ZNF33B recruits METTL14 to stabilize the METTL3‐METTL14 m6A methyltransferase complex, leading to increased m6A modification of host transcripts, including Trim25 mRNA. ZNF33B selectively binds m6A‐modified sites on Trim25 mRNA and accelerates its decay, resulting in reduced TRIM25 protein abundance.
Jian Du, Chunwei Li, Jiyuan Luo, Huizhi Zhang, Jinyan Zhang, Suya Wang, Huanchun Chen, Hongli Xu, Xiangmin Li, Ping Qian   +9 more
wiley   +1 more source

HLA‐DR+ Schwann Cells Generate the Protumor Cancer‐Neuron‐Immune Niche in Head and Neck Squamous Cell Carcinoma

Advanced Science, EarlyView.
Hijacked and educated by HNSCC cells, HLA‐DR+ Schwann cells lost their normal neural‐related functions but acquired immunoregulatory phenotypes to promote CD4+ T cells transform into Tregs. HLA‐DR+ Schwann cells induced a macrophage subpopulation, Il1β.
Xiaoyan Meng, Zhonglong Liu, Shijian Zhang, Luoman Gan, Liren Cao, Jingjing Sun, Lingfang Zhang, Yue He   +7 more
wiley   +1 more source

Next-generation strategies to improve safety and efficacy of adeno-associated virus-based gene therapy for hemophilia: lessons from clinical trials in other gene therapies

Haematologica
Three major directions for the global progress of adeno-associated virus (AAV) vectors for gene therapies (GT) are analyzed: a) engineering vectors to increase transgene expression; b) aligning interests of the health system with costs and challenges ...
Giovanni Di Minno, Wolfgang Miesbach, Giancarlo Castaman, Flora Peyvandi   +3 more
doaj   +1 more source

Antigen-selective modulation of AAV immunogenicity with tolerogenic rapamycin nanoparticles enables successful vector re-administration

Nature Communications, 2018
Immunogenicity of AAV vectors renders repeated AAV dosing ineffective. Here the authors show that coadministration of nanoparticle-encapsulated rapamycin overcomes AAV immunogenicity through Treg induction, enabling efficient AAV redosing in mice and ...
Amine Meliani, Florence Boisgerault, Romain Hardet, Solenne Marmier, Fanny Collaud, Giuseppe Ronzitti, Christian Leborgne, Helena Costa Verdera, Marcelo Simon Sola, Severine Charles, Alban Vignaud, Laetitia van Wittenberghe, Giorgia Manni, Olivier Christophe, Francesca Fallarino, Christopher Roy, Alicia Michaud, Petr Ilyinskii, Takashi Kei Kishimoto, Federico Mingozzi   +19 more
doaj   +1 more source