Examining peak height ratios in low template DNA samples with and without sampling using a single-tube extraction protocol [PDF]
, 2015 The developments of the polymerase chain reaction (PCR) and the short tandem repeat multiplex kits increased the ease and lowered the time and sample quantity required for deoxyribonucleic acid (DNA) typing compared to previous methods.
Nguyen, Thutrang Thi
core +1 more source
FEBS Letters, EarlyView.We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Carp Edema Virus Infection Is Associated With Severe Metabolic Disturbance in Fish
Frontiers in Veterinary Science, 2021 Significant mortalities associated with emerging viral diseases are challenging the economy of common carp aquaculture. As such, there is an increased need to disentangle how infected fish cope with progressive disease pathology and lose the ability for ...
Jiri Pikula +14 more
doaj +1 more source
Prediction of Acid-Base and Potassium Imbalances in Intensive Care Patients Using Machine Learning Techniques [PDF]
, 2023 Ratchakit Phetrittikun +4 more
openalex +1 more source
Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source
Infusing sodium bicarbonate suppresses hydrogen peroxide accumulation and superoxide dismutase activity in hypoxic-reoxygenated newborn piglets. [PDF]
PLoS ONE, 2012 The effectiveness of sodium bicarbonate (SB) has recently been questioned although it is often used to correct metabolic acidosis of neonates. The aim of the present study was to examine its effect on hemodynamic changes and hydrogen peroxide (H(2)O(2 ...
Jiang-Qin Liu +5 more
doaj +1 more source
Diversity and complexity in neural organoids
FEBS Letters, EarlyView.Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley +1 more source
Critical Response Training for Surgical Interns
MedEdPORTAL, 2010 Introduction In order to provide safe, timely, and effective care for our patients, it is imperative that surgical interns are prepared to recognize and manage acute postoperative complications, particularly those emergencies which are life-threatening ...
Mara Antonoff +3 more
doaj +1 more source
Rates of sustainable forest harvest depend on rotation length and weathering of soil minerals [PDF]
, 2014 Removals of forest biomass in the northeastern US may intensify over the coming decades due to increased demand for renewable energy. For forests to regenerate successfully following intensified harvests, the nutrients removed from the ecosystem in the ...
Blum, Joel D. +3 more
core +2 more sources