Results 61 to 70 of about 31,149 (219)

Three‐Dimensional Influence of Photobiomodulation on Adipose‐Derived Stem Cell Behavior

Journal of Biophotonics, EarlyView.
Photobiomodulation (PBM) at 825 nm is evaluated on adipose‐derived stem cells cultured in 2D and 3D models. PBM enhanced cell viability and proliferation, with 5 J/cm2 showing strong effects in 2D, and 10 J/cm2 in 3D spheroids. Results show 3D provides a more physiologically relevant platform for assessing PBM‐induced regenerative responses.
Precious Earldom Mulaudzi, Heidi Abrahamse, Anine Crous   +2 more
wiley   +1 more source

Probing Internalization Effects and Biocompatibility of Ultrasmall Zirconium Metal-Organic Frameworks UiO-66 NP in U251 Glioblastoma Cancer Cells

Nanomaterials, 2018
The synthesis of ultrasmall UiO-66 nanoparticles (NPs) with an average size of 25 nm, determined by X-ray powder diffraction and electron microscopies analysis, is reported.
Cataldo Arcuri, Lorenzo Monarca, Francesco Ragonese, Carmen Mecca, Stefano Bruscoli, Stefano Giovagnoli, Rosario Donato, Oxana Bereshchenko, Bernard Fioretti, Ferdinando Costantino   +9 more
doaj   +1 more source

Microfluidic Intracytoplasmic Sperm Injection (MICSI): A Novel Platform for Sperm Isolation, Selection, and Injection Into Oocytes

Andrology, EarlyView.
ABSTRACT Background Infertility is a pressing global health concern, affecting one in six couples worldwide. The failure rate for assisted reproductive technologies (ART) cycles remains at approximately 78%, with limited improvements often attributed to a lack of technological innovation.
Mehran Dabiri, Dale Mark Goss, Steven Alexander Vasilescu, Majid Ebrahimi Warkiani   +3 more
wiley   +1 more source

Measuring Propidium Iodide Brightness as a Function of Viability: A High‐Throughput Quantitative Assessment of Hepatic Spheroids

Artificial Organs, EarlyView.
The graphical abstract illustrates our rapid PI‐based spheroid viability assay: hepatocyte spheroids are formed and stained with AO/PI, imaged after a 15‐min incubation, and analyzed for PI brightness normalized to area. This metric correlates strongly with functional urea/DNA output, validating PI intensity as a fast, reliable indicator of spheroid ...
Michael G. Megaly, Diane Tobolt, Bat‐Erdene Namsrai, Benjamin Fisher, Anthony An‐Fa Dahm Chen, Joseph Sushil Rao, Srivasupradha Ramesh, Anudari Chimedtseren, Mark Clemens, Charles Y. Lee, John C. Bischof, Erik B. Finger   +11 more
wiley   +1 more source

Lysosomal Stability Assay

Bio-Protocol, 2014
This assay makes use of the dye Acridine Orange (AO) to determine the stability of lysosomes in living cells upon exposure to a confocal microscope laser. AO is a lipophilic amine that readily diffuses into cells (Figure 1). Inside the cell it enters the
Nikolaj Petersen, Thomas Kirkegaard, Marja Jäättelä   +2 more
doaj   +1 more source

Chromosomes of Aganaspis daci (Weld, 1951) and a review of known karyotypes of the family Figitidae (Hymenoptera) [PDF]

Comparative Cytogenetics
The parasitoid wasp Aganaspis daci (Weld, 1951) is a natural enemy of several tephritid fruit flies and was recently detected for the first time in Italy.
Vladimir E. Gokhman, Feliciana Pica, Fortuna Miele, Francesco Nugnes   +3 more
doaj   +3 more sources

Tau‐targeting active immunotherapy slows progression and reduces pathology in mouse models of tauopathy

Brain Pathology, EarlyView.
The efficacy of the novel anti‐tau active immunotherapy, p5555kb, was tested using two mouse models of tau pathology. p5555kb inoculation increased the survival rate and reduced tau pathology in tau‐overexpressing P301L mice and decreased tau seeding in the brains of C57BL/6 mice injected with human‐purified Alzheimer's disease tau.
Christopher M. Brown, Jeanne K. Brooks, Louise Kelly, Madeline M. Vroom, Matthew Longo, Jean‐Cosme Dodart, Roxana Carare, Justin D. Boyd   +7 more
wiley   +1 more source

Cyclin‐dependent kinase 13 is indispensable for normal mouse heart development

Journal of Anatomy, Volume 246, Issue 4, Page 616-630, April 2025.
Congenital heart disease (CHD) is the most common defect in live births. The role of cyclin‐dependent kinase (CDK13) in cardiogenesis and CHD was studied using a transgenic mouse model (Cdk13tm1b) carrying deletion of exons 3 and 4, causing loss of function.
Qazi Waheed‐Ullah, Anna Wilsdon, Aseel Abbad, Sophie Rochette, Frances Bu'Lock, Asma Ali Saed, Marc‐Phillip Hitz, J. David Brook, Siobhan Loughna   +8 more
wiley   +1 more source

Mutagenic and Induced Antibiotic Resistance of Acridine Dye on E. Coli and Proteus mirabilis Isolated from Clinical Samples.

Sokoto Journal of Medical Laboratory Science, 2019
Acridine orange has been associated with chemical mutagenic compound that can cause an increase in the frequency of mutations. Its mutagenic influence varies with its potency because of its ability to penetrate the cell, its reactivity with DNA and its ...
Igwe, J.C.* , Parom, S.K. , Onaolapo, J.A., Ehnimidu, J.O. , Afolabi-Balogun, N.B. , Olajumoke, B.R.
doaj  

The Effect of Coumarin Derivatives(compounds) on the Vibrio cholerae Isolates from Different Clinical Iraqi Sources

Iraqi Journal of Pharmaceutical Sciences, 2017
From a large number of bacterial samples collected from different hospital in Iraq in central  health laboratory ,only ten isolates were identified primary as Vibrio.
Fatima .R.Abdul, Nehad .A.Taher, Ashraf S.Hassan, Enaam H. Batah   +3 more
doaj   +1 more source