Results 111 to 120 of about 24,732 (236)
Acrosome biogenesis: Revisiting old questions to yield new insights
, 2011 The acrosome is a unique membranous organelle located over the anterior part of the sperm nucleus that is highly conserved throughout evolution. This acidic vacuole contains a number of hydrolytic enzymes that, when secreted, help the sperm penetrate the
C. Paiardi +3 more
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Zona pellucida mediated acrosome reaction and sperm morphology
, 1997 Sperm samples from 29 men randomly selected from the andrology laboratory, were used to evaluate acrosome reaction response to solubilized human zona pellucida. Capacitated sperm samples were exposed to a solution containing 2 zona pellucidae (ZP) per μl
Habenicht U.-F. +4 more
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Studies on male infertility--acrosome reaction of the human sperm incubated in vitro [PDF]
, 1985 triple-stain techniqueを用いヒト精子acrosome reactionを観察した.1) mBWW中では培養6時間後まではacrosome reaction陽性精子の割合は漸増した.2) human serum albuminを含まない培養液中ではacrosome reactionはおこらなかった.Caイオン, Ca ionophore A23187, trypsinにはacrosome reactionの誘発作用を認めた.3)不妊患者精子の中で乏精子症患者の精子や運動率が20 ...
岡田, 弘
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, 2013 Acrosome reaction was carried out under the standard assay conditions and the cells after staining with Rose Bengal were observed under microscope at 1,000× magnification. Sperm cells treated with anti-p14 antibody.
Pinki Nandi (334552) +3 more
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CellsCryopreservation of sperm is a resource used for artificial insemination. In the case of bovines, it ensures the reproduction of animals through cells from males with a highlighted genetic background. The fertilisation capacity of sperm cells is achieved
Maia A. Rodríguez +3 more
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ADVANCED STUDIES ON IMPROVING SHEEP FERTILITY BY USING ARTIFICIAL MEANS OF REPRODUCTION [PDF]
Scientific Papers Series : Management, Economic Engineering in Agriculture and Rural Development, 2014 Artificial insemination (AI) in livestock is used to optimize reproduction efficiency. Compared to other semenpreservation methods, cryopreservation is an established industry used worldwide for performing AI.
Mostafa A.R. IBRAHIM +7 more
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Immunolocalisation of PDE10A in acrosome intact and acrosome reacted ejaculated bull spermatozoa.
, 2016 Washed spermatozoa were fixed with formaldehyde and permeabilized with Triton X-100. They were next incubated overnight with a monoclonal anti-PDE10A antibody (clone 3G9; A-F) or a commercial non-immune mouse IgG at the same concentration (G-I). Panels A,
Loïze Maréchal (3070584) +5 more
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Relationship between UBPy and the Endosome System in Acrosome Biogenesis
, 2009 Summary. One of the major events that characterize spermiogenenesis is the formation of the acrosome. Acrosome-less spermatozoa are infertile; in human, globozoospermia is a rare but severe disorder in male infertility. The acrosome formation is far more
G. Berruti
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Alkalinization of Acrosome Measured by GFP as a pH Indicator and Its Relation to Sperm Capacitation
, 2001 We previously targeted EGFP (a mutant of green fluorescent protein) to the lumen of the mouse sperm acrosome and reported the time course of EGFP release during the acrosome reaction. In the study reported here, we estimated the pH within the mouse sperm
Nakanishi, Tomoko +4 more
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Targeting and fusion proteins during mammalian spermiogenesis
Biological Research, 2001 Regulated exocytosis is controlled by internal and external signals. The molecular machinery controlling the sorting from the newly synthesized vesicles from the Golgi apparatus to the plasma membrane play a key role in the regulation of both the number ...
JOÃO RAMALHO-SANTOS, RICARDO D. MORENO
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