Results 141 to 150 of about 6,799 (175)

Actin Depolymerizing Factor

2000
Unlike other actin-binding proteins, actin depolymerizing factor (ADF) binds to both filamentous and globular actin, and causes the rapid depolymerization of actin filaments. ADF localizes to sites of active actin dynamics, including growing maize root hairs.
Christopher J Staiger, Kovář David R, Staiger Christopher J   +2 more
exaly   +2 more sources

Analysis of the interactions of actin depolymerizing factor with G- and F-actin

Biochemistry, 1993
Chick actin depolymerizing factor (ADF) is an actin binding protein previously shown to rapidly depolymerize actin filaments in vitro, yielding a 1:1 complex of ADF and actin monomer. Here we show that ADF protects actin monomer from denaturation by EDTA by inhibiting the exchange of actin-bound nucleotide.
S M, Hayden, P S, Miller, A, Brauweiler, J R, Bamburg   +3 more
openaire   +2 more sources

The actin-depolymerizing factor destrin has an actin-stabilizing domain

Biochemistry and Cell Biology, 2001
Destrin is a 19 kDa actin-depolymerizing protein of the ADF–cofilin family. Destrin was digested with trypsin to a structurally stable 9.2 kDa fragment that contains the actin-binding sequence. The purified 9.2 kDa fragment has an actin filament stabilizing activity, rather than an actin filament depolymerizing activity. The deleted region is probably
K, Tokuraku, S, Okamoto, M, Katsuki, H, Nakagawa, S, Kotani   +4 more
openaire   +2 more sources

Cellular Factors Mediate Cadmium-Dependent Actin Depolymerization

Toxicology and Applied Pharmacology, 1996
Cadmium exposure produces depolymerization of the actin cytoskeleton in several cultured cell lines, and we have previously shown that in renal mesangial cells this effect is caused by picomolar concentrations of cytosolic Cd2+. The present study was undertaken to explore further the basis of this action of Cd2+.
Z, Wang, D M, Templeton
openaire   +2 more sources

NMR solution structures of actin depolymerizing factor homology domains [PDF]

Protein Science, 2009
AbstractActin is one of the most conserved proteins in nature. Its assembly and disassembly are regulated by many proteins, including the family of actin‐depolymerizing factor homology (ADF‐H) domains. ADF‐H domains can be divided into five classes: ADF/cofilin, glia maturation factor (GMF), coactosin, twinfilin, and Abp1/drebrin.
Satoru Watanabe, Alexander K Goroncy, Takanori Kigawa   +2 more
exaly   +3 more sources

Actin Depolymerizing Factor Is a Component of Slow Axonal Transport

Journal of Neurochemistry, 1992
Abstract: We examined the low molecular weight proteins transported with actin in the chicken sciatic nerve after injection of [35S]methionine into the lumbar spinal cord. A prominent component of slow axonal transport with apparent molecular mass 19 kDa comigrated on two‐dimensional gels with chicken actin depolymerizing factor (ADF), previously ...
J J, Bray, P, Fernyhough, J R, Bamburg, D, Bray   +3 more
openaire   +2 more sources

Profilin and actin-depolymerizing factor: modulators of actin organization in plants

Trends in Plant Science, 1997
A striking feature of the eukaryotic actin cytoskeleton is its ability to undergo dramatic reorganization in response to internal and environmental stimuli. In plant cells, several cellular processes depend on, or are coincident with, reorganization of the actin cytoskeleton, including division, differentiation, light-induced plastid migration, wound ...
Christopher J. Staiger, Bryan C. Gibbon, David R. Kovar, Laura E. Zonia   +3 more
openaire   +1 more source

Human actin depolymerizing factor mediates a pH-sensitive destruction of actin filaments

Biochemistry, 1993
ADF (actin depolymerizing factor) is an M(r) 19,000 actin-binding protein present in many vertebrate tissues and particularly abundant in neuronal cells. We have cloned human ADF and here show it to be identical in sequence to porcine destrin. Human ADF expressed in Escherichia coli behaves like native ADF from porcine brain.
M, Hawkins, B, Pope, S K, Maciver, A G, Weeds   +3 more
openaire   +2 more sources

Regulating filopodial dynamics through actin-depolymerizing factor/cofilin

Anatomical Science International, 2004
The regulation of filopodial dynamics by neurotrophins and other guidance cues plays an integral role in growth cone pathfinding. Filopodia are F-actin-based structures that explore the local environment, generate forces and play a role in growth cone translocation.
Joseph, Fass, Scott, Gehler, Patrick, Sarmiere, Paul, Letourneau, James R, Bamburg   +4 more
openaire   +2 more sources

Proteins of the Actin Depolymerizing Factor/Cofilin Family

2008
Actin depolymerizing factor (ADF) and cofilin are the founding members of a group of structurally and functionally related actin binding proteins now collectively known as the ADF/cofilin (AC) family. AC proteins are expressed in all eukaryotic organisms, and their unique ability to bind and dynamize filamentous actin renders them essential to all ...
Janel D. Funk, James R. Bamburg
openaire   +1 more source