Results 211 to 220 of about 47,958 (242)

Improved purification of acyl carrier protein

Analytical Biochemistry, 1980
Abstract An improved method for the purification of acyl carrier protein from Escherichia coli is described. The method consists of four steps: a 2-propanol extraction, batch adsorption to DEAE-cellulose, ammonium sulfate fractionation, and acid precipitation.
C O, Rock, J E, Cronan
openaire   +2 more sources

Acyl carrier protein interacts with melittin

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1990
Acyl carrier protein (ACP) from Escherichia coli has been shown to form complexes with melittin, a cationic peptide from bee venom. ACP is a small (Mr 8847), acidic, Ca2(+)-binding protein, which possesses some characteristics resembling those of regulatory Ca2(+)-binding proteins including interaction with melittin. Complexing between melittin and ACP
Ernst-Fonberg, Mary L., Williams, Sande G., Worsham, Lesa M.S.   +2 more
openaire   +3 more sources

Desaturation of trans-octadecenoyl-acyl carrier protein by stearoyl-acyl carrier protein Δ9 desaturase

Journal of Inorganic Biochemistry, 2000
Positional isomers of mono-unsaturated 18:1-ACP have been used as substrates for stearoyl-acyl carrier protein delta9 desaturase to test whether a C-H bond abstraction from either the C-9 or C-10 position could lead to rearranged products diagnostic for the production of an allylic radical intermediate.
J A, Broadwater, B J, Laundre, B G, Fox
openaire   +2 more sources

Acyl carrier protein from E. coli. Structural characterization of short-chain acylated acyl carrier proteins by NMR

Biochemistry, 1985
Acylated acyl carrier proteins (ACPs) with acyl chain lengths of 2, 4, 6, 8, and 10 carbons were investigated by NMR and nuclear Overhauser methods at 500 MHz. Chemical shift changes of downfield aromatic and upfield, ring-current-shifted, isoleucine proton resonances monotonically vary as a function of acyl chain length with the most prominent shifts ...
K H, Mayo, J H, Prestegard
openaire   +2 more sources

Utilization of Enzymatically Phosphopantetheinylated Acyl Carrier Proteins and Acetyl−Acyl Carrier Proteins by the Actinorhodin Polyketide Synthase

Biochemistry, 1997
The functional reconstitution of two purified proteins of an aromatic polyketide synthase pathway, the acyl carrier protein (ACP) and holo-ACP synthase (ACPS), is described. Holo-ACPs were enzymatically synthesized from coenzyme A and apo-ACPs using Escherichia coli ACPS.
C W, Carreras, A M, Gehring, C T, Walsh, C, Khosla   +3 more
openaire   +2 more sources

The acyl carrier protein dance

Nature Chemical Biology, 2023
openaire   +2 more sources

Modifying the Thioester Linkage Affects the Structure of the Acyl Carrier Protein

Angewandte Chemie - International Edition, 2019
Tony D Davis, Terra Sztain, Michael Burkart   +2 more
exaly  

Expression, purification and characterization of the acyl carrier protein phosphodiesterase from Pseudomonas Aeruginosa

Protein Expression and Purification, 2010
Zhao-Xun Liang, Feng Rao
exaly  

Structural and Biochemical Analysis of Protein–Protein Interactions Between the Acyl‐Carrier Protein and Product Template Domain

Angewandte Chemie - International Edition, 2016
Kara Finzel, Anna Lisa Vagstad, Jordan Meier   +2 more
exaly  

Structure-based analysis of the molecular interactions between acyltransferase and acyl carrier protein in vicenistatin biosynthesis

Proceedings of the National Academy of Sciences of the United States of America, 2016
Fumitaka Kudo, Tadashi Eguchi, Akimasa Miyanaga   +2 more
exaly