Results 251 to 260 of about 80,359 (315)

Characterization of the Cubamyces Menziesii Terpenome

ChemBioChem, EarlyView.
The genome of Cubamyces menziesii reveals 18 putative sesquiterpene cyclase genes. These genes are cloned and expressed in Escherichia coli, yielding 10 active enzymes. Using farnesyl diphosphate as substrate, the enzymes are analyzed, and the products characterized after bioconversion, uncovering diverse sesquiterpene structures. This study highlights
Létitia Leydet, Julie Couillaud, Agnès Amouric, Elise Courvoisier‐Dezord, Carole Avesque, Thierry Giardina, Mireille Attolini, Pierre Rousselot‐Pailley, Katia Duquesne, Marie‐Noelle Rosso, Gilles Iacazio   +10 more
wiley   +1 more source

Direct structural analysis of a single acyl carrier protein domain in fatty acid synthase from the fungus Saccharomyces cerevisiae. [PDF]

Commun Biol
Samani EK, Chen AC, Lou JW, Dai DL, Keszei AFA, Tan G, Boone C, Grininger M, Mazhab-Jafari MT.   +8 more
europepmc   +1 more source

Activity Mapping the Acyl Carrier Protein: Elongating Ketosynthase Interaction in Fatty Acid Biosynthesis. [PDF]

Biochemistry, 2020
Mindrebo JT, Misson LE, Johnson C, Noel JP, Burkart MD.   +4 more
europepmc   +1 more source

Engineering Δ9-16:0-Acyl Carrier Protein (ACP) Desaturase Specificity Based on Combinatorial Saturation Mutagenesis and Logical Redesign of the Castor Δ9-18:0-ACP Desaturase [PDF]

, 2001
Edward Whittle, John Shanklin
openalex   +1 more source

Activity‐Enhancing Mutations in an LmrR‐Based Artificial Metalloenzyme Destabilize the Protein Scaffold and Alter its Conformational Plasticity

ChemBioChem, EarlyView.
The protein LmrR is a versatile scaffold to design artificial metalloenzymes. Here, we show that the M8D/A92E (DE) mutations that result in a much more efficient catalyst, destabilize the protein dimerization interface and alter the conformation landscape when binding metal cofactor and substrates are shown.
Adil A. Safeer, Fabrizio Casilli, Wouter Beugelink, Gerard Roelfes, Marc Baldus, Hugo van Ingen   +5 more
wiley   +1 more source

Kidney triglyceride accumulation in the fasted mouse is dependent upon serum free fatty acids [PDF]

, 2017
Abumrad, Nada A., al, et, Cifarelli, Vincenza   +2 more
core   +2 more sources

Purification and Biochemical Characterization of theMycobacterium tuberculosis β-Ketoacyl-acyl Carrier Protein Synthases KasA and KasB [PDF]

, 2001
Merrill L. Schaeffer, Gautam Agnihotri, Craig Volker, Howard Kallender, Patrick J. Brennan, John T. Lonsdale   +5 more
openalex   +1 more source

Selective Lysine Ubiquitination Using Activated Phenol Esters

ChemBioChem, EarlyView.
Ubiquitination of both peptides and proteins using a novel water‐soluble acylation reagent, based on a 2,4‐dichloro‐6‐sulfonic acid phenol ester, is presented. At alkaline pH, fast lysine ubiquitination is observed on model peptide FLYRANK, and its regioselectivity is confirmed using controls.
Halana C. Vlaming, Yara Huppelschoten, Rayman T. N. Tjokrodirijo, Peter A. van Veelen, Francesca D’Amico, Kim B. Jensen, Jens Buchardt, Thomas E. Nielsen, Bhavesh Premdjee, Gerbrand J. van der Heden van Noort   +9 more
wiley   +1 more source

Design, synthesis and computational studies of new azaheterocyclic coumarin derivatives as anti-Mycobacterium tuberculosis agents targeting enoyl acyl carrier protein reductase (InhA). [PDF]

RSC Adv
Batran RZ, Sabt A, Dziadek J, Kassem AF.
europepmc   +1 more source

Purification and characterization of acyl-acyl carrier protein synthetase from oleaginous yeast and its role in triacylglycerol biosynthesis [PDF]

, 2001
Akanksha Gangar, Anjali A. Karande, Ram Rajasekharan   +2 more
openalex   +1 more source