Results 301 to 310 of about 80,359 (315)
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Protein Expression and Purification, 2000
Escherichia coli acyl carrier protein (ACP) contains a single tyrosine residue at position 71. The combined o-nitration of apo-ACP Y71 by tetranitromethane and reduction to 3-aminotyrosyl-apo-ACP were performed to introduce a specific site for attachment of a dansyl fluorescent label.
Brian G. Fox +2 more
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Escherichia coli acyl carrier protein (ACP) contains a single tyrosine residue at position 71. The combined o-nitration of apo-ACP Y71 by tetranitromethane and reduction to 3-aminotyrosyl-apo-ACP were performed to introduce a specific site for attachment of a dansyl fluorescent label.
Brian G. Fox +2 more
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Behavior of acyl carrier proteins on western blots
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1990Acyl carrier proteins (ACPs) from Escherichia coli and Euglena were analyzed on Western blots using rabbit antibodies raised against E. coli ACP. Euglena ACP, unlike that from E. coli, behaves upon electrophoresis under denaturing conditions as its size would predict.
Worsham, Lesa M. +2 more
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An approach to the semisynthesis of acyl carrier protein
Bioorganic Chemistry, 1981Abstract A scheme has been devised for the preparation of semisynthetic derivatives of acyl carrier protein (ACP). Acetylated synthetic ACP1–6 is coupled via its activated pentachlorophenol ester to native ACP (7–77), which had previously been acetylated and converted to the S-5′-dithiobis(2-nitrobenzoate)(DTNB) derivative. Removal of the DTNB moiety
David R. K. Harding +3 more
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Proteins: Structure, Function, and Genetics, 1997
Acyl carrier proteins (ACPs) from spinach and from Escherichia coli have been used to demonstrate the utility of proton NMR for comparison of homologous structures. The structure of E. coli ACP had been previously determined and modeled as a rapid equilibrium among multiple conformational forms (Kim and Prestegard, Biochemistry 28:8792-8797, 1989 ...
Yangmee Kim +3 more
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Acyl carrier proteins (ACPs) from spinach and from Escherichia coli have been used to demonstrate the utility of proton NMR for comparison of homologous structures. The structure of E. coli ACP had been previously determined and modeled as a rapid equilibrium among multiple conformational forms (Kim and Prestegard, Biochemistry 28:8792-8797, 1989 ...
Yangmee Kim +3 more
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The primary structure of spinach acyl carrier protein
Archives of Biochemistry and Biophysics, 1984Acyl carrier protein (ACP) from spinach leaves has been purified to homogeneity by high-performance liquid chromatography with an anion-exchange column. The amino acid sequence of one major ACP in spinach leaves, ACP-I, has been determined by automated Edman degradation. It consists of the following 82 amino acids: (sequence in text). Sequencing of the
Tsung Min Kuo, John B. Ohlrogge
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[12] Acyl carrier protein synthetase
1975Publisher Summary This chapter discusses the determination of acyl carrier protein (ACP) synthetase. The conversion of apo-ACP to ACP is measured in a two-stage assay. In the first-stage synthesis of ACP is allowed to proceed, catalyzed by ACP synthetase. This stage is terminated, and the amount of ACP synthesized is determined in the second stage by
P. Roy Vagelos +2 more
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Solution Structure of Acyl Carrier Protein
1982The role of acyl carrier protein (ACP) in the biosynthesis of membrane lipid precursors is well established (for review see Vagelos, 1971, 1973; Prescott and Vagelos, 1972). ACP and its thioesters interact specifically with at least a dozen enzymes of fatty acid biosynthesis in Escherichia coli, and acyl-ACPs are substrates for the sn-glycerol-3 ...
John E. CronanJr., Charles O. Rock
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5 Acyl Group Transfer (Acyl Carrier Protein)
1973Publisher Summary This chapter discusses acyl group transfer or acyl carrier protein (ACP). This protein was discovered as a heat-stable protein in extracts of Clostridium kluyveri where it was shown to function in the condensation reaction of fatty acid biosynthesis.
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Chemical reactions of an acyl carrier protein
1996This thesis was digitised by the British Library from microfilm. You can acquire a single copy of this thesis for research purposes by clicking on the padlock icon on the thesis file. Please be aware that the text in the supplied thesis pdf file may not be as clear as text in a thesis that was born digital or digitised directly from paper due to the ...
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