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Adaptive laboratory evolution boosts Yarrowia lipolytica tolerance to vanillic acid
Journal of Biotechnology, 2023Microbial tolerance to lignocellulose-derived inhibitors, such as aromatic acids, is critical for the economical production of biofuels and biochemicals. Here, adaptive laboratory evolution was applied to improve the tolerance of Yarrowia lipolytica to a representative aromatic acid inhibitor vanillic acid. The transcriptome profiling of evolved strain
Yuanyuan Sha +7 more
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Adaptive Laboratory Evolution for Strain Engineering
2013Complex phenotypes, such as tolerance to growth inhibitors, are difficult to rationally engineer into industrial model organisms due our poor understanding of their underlying molecular mechanisms. Adaptive evolution circumvents this issue by exploiting the linkage between growth rate and inhibitor resistance to select for mutants with enhanced ...
James, Winkler +2 more
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Adaptive laboratory evolution of Yarrowia lipolytica improves ferulic acid tolerance
Applied Microbiology and Biotechnology, 2021Yarrowia lipolytica strain is a promising cell factory for the conversion of lignocellulose to biofuels and bioproducts. Despite the inherent robustness of this strain, further improvements to lignocellulose-derived inhibitors toxicity tolerance of Y. lipolytica are also required to achieve industrial application.
Zedi Wang +8 more
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Growth-Coupled Carotenoids Production Using Adaptive Laboratory Evolution
2018Adaptive laboratory evolution is a powerful technique for strain development. However, the target phenotypes using this strategy have been limited by the required coupling of the phenotype-of-interest with fitness or survival, and thus adaptive evolution is generally not used to improve product formation. If the desired product confers a benefit to the
Reyes, Luis, Kao, Katy
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Microbe Magazine, 2011
We now can observe evolution in laboratory experiments, define its dynamics, and determine the genetic changes underlying new phenotypes. What happens in such experiments may not be directly applicable to evolution in natural habitats, however, unless particular habitats can be accurately reproduced in the laboratory.
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We now can observe evolution in laboratory experiments, define its dynamics, and determine the genetic changes underlying new phenotypes. What happens in such experiments may not be directly applicable to evolution in natural habitats, however, unless particular habitats can be accurately reproduced in the laboratory.
openaire +1 more source
Developing Synthetic Methylotrophs by Metabolic Engineering-Guided Adaptive Laboratory Evolution
2022Methanol is a promising alternative feedstock for biomanufacturing. However, natural platform industrial microorganisms such as Escherichia coli and Corynebacterium glutamicum cannot assimilate methanol. Although some methanol assimilation pathways differ from the typical sugar metabolism by only a few enzymes, engineering platform microorganisms to ...
Yu, Wang, Ping, Zheng, Jibin, Sun
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Temperature adaptation of enzymes: Lessons from laboratory evolution
2001Publisher Summary This chapter outlines the evolutionary protein design methods that are used to help uncover the molecular basis for temperature adaptation in enzymes. The chapter explains how temperature affects protein stability and enzyme activity.
Wintrode, Patrick L., Arnold, Frances H.
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2015
We now can observe evolution in laboratory experiments, define its dynamics, and determine the genetic changes underlying new phenotypes. What happens in such experiments may not be directly applicable to evolution in natural habitats, however, unless particular habitats can be accurately reproduced in the laboratory.
openaire +1 more source
We now can observe evolution in laboratory experiments, define its dynamics, and determine the genetic changes underlying new phenotypes. What happens in such experiments may not be directly applicable to evolution in natural habitats, however, unless particular habitats can be accurately reproduced in the laboratory.
openaire +1 more source
Adaptive Laboratory Evolution for Enhanced Carotenoid Production in Microalgae
2018In order to produce natural pigments with competitive prices, algal strains employed in industrial production need to be improved for increasing the productivity of valuable metabolites, thereby reducing the overall production cost. Adaptive laboratory evolution (ALE) is a traditional method for strain improvement, which has been effectively utilized ...
Yixi, Su +4 more
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An Adaptive Laboratory Evolution Method to Accelerate Autotrophic Metabolism
2018Adaptive laboratory evolution (ALE) is an approach enabling the development of novel characteristics in microbial strains via the application of a constant selection pressure. This method is also an efficient tool to acquire insights on molecular mechanisms responsible for specific phenotypes.
Tian, Zhang, Pier-Luc, Tremblay
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