Design and analysis strategies for robust microbiome ageing research
FEBS Letters, EarlyView.The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
The numerical simulation tool for the MAORY multiconjugate adaptive optics system [PDF]
, 2016 The Multiconjugate Adaptive Optics RelaY (MAORY) is and Adaptive Optics module to be mounted on the ESO European-Extremely Large Telescope (E-ELT).
XOMPERO, MARCO +33 more
core +1 more source
Size of the halo of the adaptive optics PSF
, 2022 Paper 844725It is a widely accepted conjecture that the width of the incoherent halo in an adaptive optics point-spread function (PSF) should decrease with the level of correction.
Gladysz, Szymon +7 more
core +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Insights into Sickle Cell Disease through the Retinal Microvasculature
Ophthalmology Science, 2022 Purpose: Clinical OCT angiography (OCTA) of the retinal microvasculature offers a quantitative correlate to systemic disease burden and treatment efficacy in sickle cell disease (SCD).
Alexander Pinhas, MD +12 more
doaj +1 more source
An experimental Rayleigh laser guide star ground layer adaptive optics system for the William Herschel telescope [PDF]
, 2005 The design of an experimental ground layer adaptive optics (GLAO) system that uses a low altitude Rayleigh laser guide star as a wavefront reference source is presented.
Morris, T J, Morris, T. J.
core
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
All-optical recording and stimulation of retinal neurons in vivo in retinal degeneration mice. [PDF]
PLoS ONE, 2018 Here we demonstrate the application of a method that could accelerate the development of novel therapies by allowing direct and repeatable visualization of cellular function in the living eye, to study loss of vision in animal models of retinal disease ...
Soon Keen Cheong +3 more
doaj +1 more source
A Local Threshold Checkerboard Algorithm for Adaptive Optics System With a Plenoptic Sensor
IEEE Photonics Journal, 2022 Adaptive optics systems will encounter challenges under strong atmospheric turbulence conditions. Recently, a modified approach based on plenoptic sensor has attracted considerable attention.
Zhichong Wang +5 more
doaj +1 more source
Discerning protein pools by selective staining with self‐labeling tags
FEBS Letters, EarlyView.Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley +1 more source