Results 311 to 320 of about 301,764 (392)

A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells

Angewandte Chemie, EarlyView.
Photoswitchable fluorophores are critical for advanced bioimaging. Here, we develop a photoswitchable self‐labeling HaloTag that can reversibly modulate the emission of a bound fluorogenic dye via a light‐induced conformational change. In living cells, this tunable chemigenetic system enables precise spatiotemporal activation and control of emitter ...
Franziska Walterspiel, Begoña Ugarte‐Uribe, Jonas Weidenhausen, Merrin Vincent, Kaarjel K. Narayanasamy, Anna Dimitriadi, Arif Ul Maula Khan, Martin Fritsch, Christoph W. Müller, Timo Zimmermann, Claire Deo   +10 more
wiley   +1 more source

A Covalent and Modulable Inhibitor of the Tubulin-Microtubule System: Insights Into the Mechanism of Cacalol. [PDF]

Chem Biol Drug Des
López-López E, Medina-Franco JL, Salinas-Arellano E, Ardila-Fierro KJ, Pardo-Novoa JC, Del Río RE, Cerda-García-Rojas CM.   +6 more
europepmc   +1 more source

Cupin‐Type Dimethylsulfoniopropionate Lyase from Pelagibacter ubique (DddKPu) Catalyzes Aza‐Michael Addition of Primary and Secondary Amines to Acrylic Acid

Angewandte Chemie, EarlyView.
A dimethylsulfoniopropionate lyase (DddK) was identified as an efficient aza‐Michaelase for the addition of amines to acrylate. Screening 74 amines with wild‐type DddK and its W26G variant yielded N‐substituted and N,N‐disubstituted β‐amino acids in 30%–93% isolated yield.
Diletta Arceri, Angela Mourelle, Teodor Parella, Jordi Bujons, Carlos J. Moreno, Pere Clapés   +5 more
wiley   +1 more source

Isotopologue Ratios Identify ¹³C-Depleted Biomarkers in Environmental Samples Impacted by Methane Turnover. [PDF]

Rapid Commun Mass Spectrom
Groninga J, Lipp J, Song M, Hinrichs KU.
europepmc   +1 more source

On‐Target Photoassembly of Pyronin Dyes for Super‐Resolution Microscopy

Angewandte Chemie, EarlyView.
We report a new class of diaryl ether and diaryl silane compounds bearing hydrazone substituents that, upon irradiation, cyclize to a fluorescent (silicon−)pyronin dye. After optimizing their photophysical properties, we introduced reactive tags into their structure for biological labeling, and demonstrated their utility in STED, PALM, and MINFLUX ...
Gergely Knorr, Mariano L. Bossi, Stefan W. Hell   +2 more
wiley   +1 more source

Thymines opposite to bulky aristolactam-DNA adducts in duplex DNA are not targeted by human thymine-DNA glycosylase. [PDF]

PeerJ
Manapkyzy D, Zhamanbayeva G, Sidorenko V, Bonala R, Johnson F, Matkarimov BT, Zharkov D, Saparbaev MK, Taipakova S.   +8 more
europepmc   +1 more source

Reduced Pressure Ionization Enhances Native Mass Spectrometry of Proteins and Protein Complexes

Angewandte Chemie, EarlyView.
Reduced pressure for electrospray ionization of native proteins narrows peak widths and increases signal. This straightforward add‐on to existing native MS platforms improves performance in challenging buffers and enables detection of protein interactions at concentrations as low as 50 nM, without significantly disrupting structure as confirmed by ...
Jake P. Violi, Chong Zhang, William A. Donald   +2 more
wiley   +1 more source

HR-MS Analysis of the Covalent Binding of Edaravone to 5-Formylpyrimidine Bases and a DNA Oligonucleotide Containing a 5-Formylcytidine Residue. [PDF]

Rapid Commun Mass Spectrom
Regnault R, Kouach M, Goossens L, Thuru X, Bailly C, Goossens JF.   +5 more
europepmc   +1 more source

Self‐Assembly, Rearrangement, and Disassembly of {Cr6} Horseshoe Oligomers

Angewandte Chemie, EarlyView.
Using a combination of ion mobility mass spectrometry (IM‐MS), tandem mass spectrometry (MS2), and low‐temperature scanning tunneling microscopy (STM), we elucidate the self‐assembly, rearrangement, and disassembly processes of polymetallic {Cr6}x (x = 1 – 5) horseshoe oligomers.
Niklas Geue, Dhaneesh Kumar, Jimin Ham, Shengpeng Huang, Grigore A. Timco, Neil A. Burton, Richard E. P. Winpenny, Kelvin Anggara, Perdita E. Barran   +8 more
wiley   +1 more source

Transcription-Coupled Nucleotide Excision Repair: A Faster Solution or the Only Option? [PDF]

Biomolecules
Khobta A, Sarmini L.
europepmc   +1 more source