Results 11 to 20 of about 487,247 (373)
Repurposing an adenine riboswitch into a fluorogenic imaging and sensing tag
Nature Chemical Biology, 2021 Sourav Dey +5 more
openalex +3 more sources
In vivo adenine base editing of PCSK9 in macaques reduces LDL cholesterol levels
Nature Biotechnology, 2021 Most known pathogenic point mutations in humans are C•G to T•A substitutions, which can be directly repaired by adenine base editors (ABEs). In this study, we investigated the efficacy and safety of ABEs in the livers of mice and cynomolgus macaques for ...
Tanja Rothgangl +31 more
semanticscholar +1 more source
Nature Biotechnology, 2022 Improved cytosine base editors are generated by phage-assisted evolution of a deoxyadenosine deaminase. Cytosine base editors (CBEs) are larger and can suffer from higher off-target activity or lower on-target editing efficiency than current adenine base
M. Neugebauer +13 more
semanticscholar +1 more source
Nature Biomedical Engineering, 2022 The viral delivery of base editors has been complicated by their size and by the limited packaging capacity of adeno-associated viruses (AAVs). Typically, dual-AAV approaches based on trans-splicing inteins have been used.
Jessie R. Davis +9 more
semanticscholar +1 more source
Nature Biotechnology, 2020 Applications of adenine base editors (ABEs) have been constrained by the limited compatibility of the deoxyadenosine deaminase component with Cas homologs other than SpCas9.
M. Richter +11 more
semanticscholar +1 more source
Trypanosoma brucei adenine-phosphoribosyltransferases mediate adenine salvage and aminopurinol susceptibility but not adenine toxicity [PDF]
International Journal for Parasitology: Drugs and Drug Resistance, 2014 African trypanosomes, like all obligate parasitic protozoa, cannot synthesize purines de novo and import purines from their hosts to build nucleic acids. The purine salvage pathways of Trypanosoma brucei being redundant, none of the involved enzymes is likely to be essential.
Lüscher, Alexandra +4 more
openaire +3 more sources
Adenine Nucleotide and Nicotinamide Adenine Dinucleotide Measurements in Plants [PDF]
Current Protocols in Plant Biology, 2020 AbstractAs the principal co‐factors of many metabolic pathways, the measurement of both adenine nucleotides and nicotinamide adenine dinucleotide provides important information about cellular energy metabolism. However, given their rapid and reversible conversion as well as their relatively low concentration ranges, it is difficult to measure these ...
Zhang, Youjun +4 more
openaire +4 more sources
Biomedicines, 2022 Chronic hypoxia drives vascular dysfunction by various mechanisms, including changes in mitochondrial respiration. Although endothelial cells (ECs) rely predominantly on glycolysis, hypoxia is known to alter oxidative phosphorylation, promote oxidative ...
Barbara Kutryb-Zajac +6 more
doaj +1 more source
Adenine base editor engineering reduces editing of bystander cytosines
Nature Biotechnology, 2021 Adenine base editors (ABEs) catalyze specific A-to-G conversions at genomic sites of interest. However, ABEs also induce cytosine deamination at the target site.
Y. Jeong +7 more
semanticscholar +1 more source