Protein-nucleic acid language model-assisted design of precise and compact adenine base editor [PDF]
Nature CommunicationsAdenine base editors (ABEs) are powerful tools for gene therapy. However, efficient version of ABEs (e.g. ABE8e) always induce excessive bystander and off-target editing events and are large in size, hindering their potential in clinical disease ...
Jingxuan Ren +10 more
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In vivo adenine base editing reverts C282Y and improves iron metabolism in hemochromatosis mice
Nature Communications, 2022 Hemochromatosis is a metabolic disorder caused by mutations in the HFE gene. Here, the authors show that a single administration of AAV8 vectors expressing an Adenine Base Editor facilitates efficient in vivo gene correction in hepatocytes and leads to ...
Alice Rovai +13 more
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Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion
Genome Biology, 2018 Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9, enabling A•T to G•C conversion at ...
Chao Li +7 more
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Adenine base editor–mediated splicing remodeling activates noncanonical splice sites [PDF]
Journal of Biological Chemistry, 2023 Qing Li
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Engineering an adenine base editor in human embryonic stem cells with minimal DNA and RNA off-target activities [PDF]
Molecular Therapy - Nucleic Acids, 2022 Zhenwu Zhang, Wanyu Tao, Xingxu Huang
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Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope. [PDF]
Nat Commun, 2020 Full author list omitted for brevity. For the full list of authors, see article.CRISPR-Cas9-associated base editing is a promising tool to correct pathogenic single nucleotide mutations in research or therapeutic settings. Efficient base editing requires
Jiang T +16 more
europepmc +3 more sources
Molecular Plant-Microbe InteractionsTowards precise genome editing, base editors have been developed by fusing catalytically compromised Cas9 with deaminase components, mediating C-to-T (cytosine base editors) or A-to-G (adenine base editors) transition.
Yi Yun Tan +10 more
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Triple base editor catalyzes saturation mutation of adenine, cytidine, and guanine
Nucleic Acids ResearchAbstract Current base editors act on a maximum of two base substrates and generate limited base conversions or transversions, hindering their applicability for inducing DNA sequence diversity. Here, we engineered a triple base editor (named ACG-BEs) using a fusion of adenine base editor with high A/C catalytic activity and evolved N ...
Youming Wu, Jinxin Wang, Guangyu Li
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Advanced ScienceThe current‐generation adenine base editor (ABE) ABE8e, which has evolved from the prokaryotic evolution system, exhibits high efficiency in mediating A‐to‐G conversion and is presumed to be promising for gene therapy.
Shenlin Hsiao +8 more
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PhieABEs: a PAM‐less/free high‐efficiency adenine base editor toolbox with wide target scope in plants [PDF]
Plant Biotechnology Journal, 2022 Dongchang Zeng, Taoli Liu, Yang Xue
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