Results 21 to 30 of about 19,262 (234)
An adenine base editor with expanded targeting scope using SpCas9‐NGv1 in rice [PDF]
Plant Biotechnology Journal, 2019 Katsuya Negishi, , Hiroaki Saika
exaly +2 more sources
Development of TALE‐adenine base editors in plants
Plant Biotechnology Journal, 2023 SummaryBase editors enable precise nucleotide changes at targeted genomic loci without requiring double‐stranded DNA breaks or repair templates. TALE‐adenine base editors (TALE‐ABEs) are genome editing tools, composed of a DNA‐binding domain from transcription activator‐like effectors (TALEs), an engineered adenosine deaminase (TadA8e), and a cytosine ...
Dingbo Zhang, Jens Boch
openaire +2 more sources
Base Editors for Citrus Gene Editing
Frontiers in Genome Editing, 2022 Base editors, such as adenine base editors (ABE) and cytosine base editors (CBE), provide alternatives for precise genome editing without generating double-strand breaks (DSBs), thus avoiding the risk of genome instability and unpredictable outcomes ...
Xiaoen Huang, Yuanchun Wang, Nian Wang
doaj +1 more source
Highly Efficient CRISPR-Mediated Base Editing in Sinorhizobium meliloti
Frontiers in Microbiology, 2021 Rhizobia are widespread gram-negative soil bacteria and indispensable symbiotic partners of leguminous plants that facilitate the most highly efficient biological nitrogen fixation in nature.
Longxiang Wang +6 more
doaj +1 more source
Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation [PDF]
Molecular Therapy, 2020 Xingxu Huang, Xinjie Wang
exaly +2 more sources
Cytosine base editor 4 but not adenine base editor generates off-target mutations in mouse embryos. [PDF]
Commun Biol, 2020 AbstractDeaminase base editing has emerged as a tool to install or correct point mutations in the genomes of living cells in a wide range of organisms. However, the genome-wide off-target effects introduced by base editors in the mammalian genome have been examined in only one study.
Lee HK +4 more
europepmc +4 more sources
Correction of DMD in human iPSC-derived cardiomyocytes by base-editing-induced exon skipping
Molecular Therapy: Methods & Clinical Development, 2023 Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene. Previously, we showed that adenine base editing (ABE) can efficiently correct a nonsense point mutation in a DMD mouse model.
Peipei Wang +5 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2023 Adenine base editors (ABEs) can mediate two transition mutations, A-to-G and T-to-C, which are suitable for repairing G·C-to-T·A pathogenic variants, the most significant human pathogenic variant.
Guo Li +8 more
doaj +1 more source
Crop Journal, 2020 The clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein (Cas) system has been widely used for genome editing. In this system, the cytosine base editor (CBE) and adenine base editor (ABE) allow generating precise ...
Feipeng Wang +7 more
doaj +1 more source
Efficient precise in vivo base editing in adult dystrophic mice
Nature Communications, 2021 Base editing is one approach used to correct mutations causing cause Duchenne muscular dystrophy (DMD), but limitations are in the requirement for a specific PAM motif and the large size beyond the packaging capacity of adeno-associated virus (AAV). Here,
Li Xu +8 more
doaj +1 more source