Results 41 to 50 of about 34,085 (286)

Directed Evolution of Adenine Base Editors with Increased Activity and Therapeutic Application [PDF]

Nature Biotechnology, 2020
The foundational adenine base editors ( e.g. ABE7.10) enable programmable C•G to T•A point mutations but editing efficiencies can be low at challenging loci in primary human cells. Here we further evolve ABE7.10 using a library of adenosine deaminase variants to create ABE8s.
Nicole M. Gaudelli, Dieter K. Lam, Holly A. Rees, Noris M. Solá-Esteves, Luis Barrera, David A. Born, Aaron Edwards, Jason M. Gehrke, Seung‐Joo Lee, Alexander Liquori, Ryan Murray, Michael S. Packer, Conrad Rinaldi, Ian M. Slaymaker, Jonathan Yen, Lauren Young, Giuseppe Ciaramella   +16 more
openalex   +3 more sources

Highly Efficient CRISPR-Mediated Base Editing in Sinorhizobium meliloti

Frontiers in Microbiology, 2021
Rhizobia are widespread gram-negative soil bacteria and indispensable symbiotic partners of leguminous plants that facilitate the most highly efficient biological nitrogen fixation in nature.
Longxiang Wang, Yuan Xiao, Xiaowei Wei, Jimin Pan, Jimin Pan, Deqiang Duanmu, Deqiang Duanmu   +6 more
doaj   +1 more source

Therapeutic adenine base editor with minimized off-target effects

Protein & Cell
Abstract Genome-wide off-target effect poses a safety risk for clinical use of adenine base editor (ABE), among which ABE8e is one of the most efficient. Genome-wide off-target analysis by two-cell embryo injection (GOTI) analysis showed that the rate of genome-wide single-nucleotide variants (SNVs) in ABE8e-edited cells was ∼30-fold ...
Yongsen Sun, Nana Yan, Feng Hu, Hongjiang Lu, Zhenrui Zuo, Chikai Zhou, Erwei Zuo   +6 more
openalex   +3 more sources

Correction of DMD in human iPSC-derived cardiomyocytes by base-editing-induced exon skipping

Molecular Therapy: Methods & Clinical Development, 2023
Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene. Previously, we showed that adenine base editing (ABE) can efficiently correct a nonsense point mutation in a DMD mouse model.
Peipei Wang, Haiwen Li, Mandi Zhu, Rena Y. Han, Shuliang Guo, Renzhi Han   +5 more
doaj   +1 more source

A novel base editor SpRY-ABE8eF148A mediates efficient A-to-G base editing with a reduced off-target effect

Molecular Therapy: Nucleic Acids, 2023
Adenine base editors (ABEs) can mediate two transition mutations, A-to-G and T-to-C, which are suitable for repairing G·C-to-T·A pathogenic variants, the most significant human pathogenic variant.
Guo Li, Yaxian Cheng, Yeqiu Li, Hongru Ma, Zhongji Pu, Sa Li, Yiqiang Zhao, Xingxu Huang, Yuan Yao   +8 more
doaj   +1 more source

Efficient precise in vivo base editing in adult dystrophic mice

Nature Communications, 2021
Base editing is one approach used to correct mutations causing cause Duchenne muscular dystrophy (DMD), but limitations are in the requirement for a specific PAM motif and the large size beyond the packaging capacity of adeno-associated virus (AAV). Here,
Li Xu, Chen Zhang, Haiwen Li, Peipei Wang, Yandi Gao, Nahush A. Mokadam, Jianjie Ma, W. David Arnold, Renzhi Han   +8 more
doaj   +1 more source

Rewriting Human History and Empowering Indigenous Communities with Genome Editing Tools. [PDF]

, 2020
Appropriate empirical-based evidence and detailed theoretical considerations should be used for evolutionary explanations of phenotypic variation observed in the field of human population genetics (especially Indigenous populations). Investigators within
Fox, Keolu, Komor, Alexis C, Rallapalli, Kartik Lakshmi   +2 more
core   +2 more sources

Developing high-efficiency base editors by combining optimized synergistic core components with new types of nuclear localization signal peptide

Crop Journal, 2020
The clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein (Cas) system has been widely used for genome editing. In this system, the cytosine base editor (CBE) and adenine base editor (ABE) allow generating precise ...
Feipeng Wang, Chengwei Zhang, Wen Xu, Shuang Yuan, Jinling Song, Lu Li, Jiuran Zhao, Jinxiao Yang   +7 more
doaj   +1 more source

CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants

Synthetic and Systems Biotechnology, 2020
CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) has been extensively exploited as a genetic tool for genome editing.
Abdullah, Zhengzheng Jiang, Xulin Hong, Shun Zhang, Ruilian Yao, Yi Xiao   +5 more
doaj   +1 more source

Development of a Simple and Quick Method to Assess Base Editing in Human Cells

Molecular Therapy: Nucleic Acids, 2020
Base editing is a form of genome editing that can directly convert a single base (C or A) to another base (T or G), which is of great potential in biomedical applications.
Xiujuan Lv, Kairui Qiu, Tianxiang Tu, Xiaoxue He, Yuchen Peng, Jinbin Ye, Junhao Fu, Ruzhi Deng, Yuqin Wang, Jinyu Wu, Changbao Liu, Junzhao Zhao, Feng Gu   +12 more
doaj   +1 more source