Cytosine but not adenine base editor generates mutations in mice [PDF]
, 2019 ABSTRACT Deaminase base editing has emerged as a tool to install or correct point mutations in the genomes of living cells in a wide range of organisms and its ultimate success therapeutically depends on its accuracy. Here we have investigated the fidelity of cytosine base editor 4 (BE4) and adenine base editor (ABE) in mouse embryos ...
Hye‐Kyung Lee +4 more
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Porous Silicon Nanoneedles Efficiently Deliver Adenine Base Editor to Correct a Recurrent Pathogenic COL7A1 Variant in Recessive Dystrophic Epidermolysis Bullosa [PDF]
Adv MaterSalman Ahmad Mustfa +10 more
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CRISPR-Pass: Gene Rescue of Nonsense Mutations Using Adenine Base Editors [PDF]
Molecular Therapy, 2019 Abstract A nonsense mutation is a substitutive mutation in a DNA sequence that causes a premature termination during translation and produces stalled proteins resulting in dysfunction of a gene. Although it usually induces severe genetic disorders, there are no definite methods for inducing read-through of premature termination codons ...
Choongil Lee +11 more
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Analysis and minimization of cellular RNA editing by DNA adenine base editors [PDF]
Science Advances, 2019 We report cellular RNA editing by adenine base editors and new ABE variants with reduced RNA editing activity.
Holly A. Rees +3 more
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Adenine base editor for knockout of proteins: A practical guide from design to analysis with updated MultiEditRbatch [PDF]
Mol Ther Nucleic AcidsElla J. Eaton +11 more
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Optimize nearly PAM-less adenine base editor to improve editing efficiency [PDF]
Jichu yixue yu linchuang, 2022 Objective To explore the effects of replacing different components of PAM-less adenine base editor (ABE) on its editing efficiency. Methods ABEmax was replaced with Tad-8e in SpRY-ABEmax to construct SpRY-ABE8e.
ZHOU Xiao-yu, WANG Xiao-yue
doaj +1 more source
TadA orthologs enable both cytosine and adenine editing of base editors [PDF]
Nature Communications, 2023 AbstractCytidine and adenosine deaminases are required for cytosine and adenine editing of base editors respectively, and no single deaminase could enable concurrent and comparable cytosine and adenine editing. Additionally, distinct properties of cytidine and adenosine deaminases lead to various types of off-target effects, including Cas9-indendepent ...
Shuqian Zhang +9 more
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Adenine base editor engineering reduces editing of bystander cytosines [PDF]
Nature Biotechnology, 2021 Adenine base editors (ABEs) catalyze specific A-to-G conversions at genomic sites of interest. However, ABEs also induce cytosine deamination at the target site. To reduce the cytosine editing activity, we engineered a commonly used adenosine deaminase, TadA7.10, and found that ABE7.10 with a D108Q mutation in TadA7.10 exhibited tenfold reduced ...
You Kyeong Jeong +7 more
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Base Editors for Citrus Gene Editing
Frontiers in Genome Editing, 2022 Base editors, such as adenine base editors (ABE) and cytosine base editors (CBE), provide alternatives for precise genome editing without generating double-strand breaks (DSBs), thus avoiding the risk of genome instability and unpredictable outcomes ...
Xiaoen Huang, Yuanchun Wang, Nian Wang
doaj +1 more source
Development of TALE‐adenine base editors in plants
Plant Biotechnology Journal, 2023 SummaryBase editors enable precise nucleotide changes at targeted genomic loci without requiring double‐stranded DNA breaks or repair templates. TALE‐adenine base editors (TALE‐ABEs) are genome editing tools, composed of a DNA‐binding domain from transcription activator‐like effectors (TALEs), an engineered adenosine deaminase (TadA8e), and a cytosine ...
Dingbo Zhang, Jens Boch
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