Results 31 to 40 of about 19,262 (234)

Human cell based directed evolution of adenine base editors with improved efficiency [PDF]

Nature Communications, 2021
Abstract Adenine base editors (ABE) are genome-editing tools that have been harnessed to introduce precise A•T to G•C conversion. However, the low activity of ABE at certain sites remains a major bottleneck that precludes efficacious applications.
Junhao Fu, Qing Li, Xiaoyu Liu, Tianxiang Tu, Xiujuan Lv, Xidi Yin, Jineng Lv, Zongming Song, Jia Qu, Jinwei Zhang, Jinsong Li, Feng Gu   +11 more
openaire   +3 more sources

CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants

Synthetic and Systems Biotechnology, 2020
CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) has been extensively exploited as a genetic tool for genome editing.
Abdullah, Zhengzheng Jiang, Xulin Hong, Shun Zhang, Ruilian Yao, Yi Xiao   +5 more
doaj   +1 more source

Development of a Simple and Quick Method to Assess Base Editing in Human Cells

Molecular Therapy: Nucleic Acids, 2020
Base editing is a form of genome editing that can directly convert a single base (C or A) to another base (T or G), which is of great potential in biomedical applications.
Xiujuan Lv, Kairui Qiu, Tianxiang Tu, Xiaoxue He, Yuchen Peng, Jinbin Ye, Junhao Fu, Ruzhi Deng, Yuqin Wang, Jinyu Wu, Changbao Liu, Junzhao Zhao, Feng Gu   +12 more
doaj   +1 more source

Protocol for targeted modification of the rice genome using base editing

STAR Protocols, 2022
Summary: Base editing is a precision genome-editing approach that is widely utilized to generate single-nucleotide variants (SNVs) in genomes. Here, we present a protocol to perform targeted adenine (A)-to-guanine (G) substitution in rice using adenine ...
Meixia Wang, Fang Yan, Huanbin Zhou
doaj   +1 more source

Examination of the Cell Cycle Dependence of Cytosine and Adenine Base Editors

Frontiers in Genome Editing, 2022
Base editors (BEs) are genome editing agents that install point mutations with high efficiency and specificity. Due to their reliance on uracil and inosine DNA damage intermediates (rather than double-strand DNA breaks, or DSBs), it has been hypothesized that BEs rely on more ubiquitous DNA repair pathways than DSB-reliant genome editing methods, which
Cameron A. Burnett, Ashley T. Wong, Carlos A. Vasquez, Colleen A. McHugh, Gene W. Yeo, Gene W. Yeo, Alexis C. Komor   +6 more
openaire   +5 more sources

Therapeutic adenine base editing of human hematopoietic stem cells

Nature Communications, 2023
Here, Liao and colleagues apply adenine base editor ABE8e and its PAM-less variant ABE8e-SpRY to β-thalassemia patient hematopoietic stem cells in the form of ribonucleoprotein complexes, resulting in efficient long-term editing and β-thalassemia ...
Jiaoyang Liao, Shuanghong Chen, Shenlin Hsiao, Yanhong Jiang, Yang Yang, Yuanjin Zhang, Xin Wang, Yongrong Lai, Daniel E. Bauer, Yuxuan Wu   +9 more
doaj   +1 more source

Targeting fidelity of adenine and cytosine base editors in mouse embryos [PDF]

Nature Communications, 2018
AbstractBase editing directly converts a target base pair into a different base pair in the genome of living cells without introducing double-stranded DNA breaks. While cytosine base editors (CBE) and adenine base editors (ABE) are used to install and correct point mutations in a wide range of organisms, the extent and distribution of off-target edits ...
Hye Kyung Lee, Michaela Willi, Shannon M. Miller, Sojung Kim, Chengyu Liu, David R. Liu, Lothar Hennighausen   +6 more
openaire   +3 more sources

Simplified adenine base editors improve adenine base editing efficiency in rice

Plant Biotechnology Journal, 2019
SummaryAdenine base editors (ABEs) have been exploited to introduce targeted adenine (A) to guanine (G) base conversions in various plant genomes, including rice, wheat and Arabidopsis. However, the ABEs reported thus far are all quite inefficient at many target sites in rice, which hampers their applications in plant genome engineering and crop ...
Kai Hua, Xiaoping Tao, Weiyi Liang, Zhaoxia Zhang, Runyu Gou, Jian‐Kang Zhu   +5 more
openaire   +2 more sources

Precise genome editing with base editors

Medical Review, 2023
Single-nucleotide variants account for about half of known pathogenic genetic variants in human. Genome editing strategies by reversing pathogenic point mutations with minimum side effects have great therapeutic potential and are now being actively ...
Liu Hongcai, Zhu Yao, Li Minjie, Gu Zhimin   +3 more
doaj   +1 more source

CRISPR/Cas9-Mediated Adenine Base Editing in Rice Genome

Rice Science, 2019
SUMMARY: Precise base editing is highly desired in plant functional genomic research and crop molecular breeding. In this study, we constructed a rice-codon optimized adenine base editor (ABE)-nCas9 tool that induced targeted A·T to G·C point mutation of
Li Hao, Qin Ruiying, Liu Xiaoshuang, Liao Shengxiang, Xu Rongfang, Yang Jianbo, Wei Pengcheng   +6 more
doaj   +1 more source