Results 31 to 40 of about 19,445 (257)

Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum). [PDF]

BMC Biol, 2022
Background: Base editors (BEs) display diverse applications in a variety of plant species such as Arabidopsis, rice, wheat, maize, soybean, and cotton, where they have been used to mediate precise base pair conversions without the collateral generation ...
Wang G, Xu Z, Wang F, Huang Y, Xin Y, Liang S, Li B, Si H, Sun L, Wang Q, Ding X, Zhu X, Chen L, Yu L, Lindsey K, Zhang X, Jin S.   +16 more
europepmc   +2 more sources

Adenine base editing-mediated exon skipping restores dystrophin in humanized Duchenne mouse model

Nature Communications
Duchenne muscular dystrophy (DMD) affecting 1 in 3500–5000 live male newborns is the frequently fatal genetic disease resulted from various mutations in DMD gene encoding dystrophin protein. About 70% of DMD-causing mutations are exon deletion leading to
Jiajia Lin, Ming Jin, Dong Yang, Zhifang Li, Yu Zhang, Qingquan Xiao, Yin Wang, Yuyang Yu, Xiumei Zhang, Zhurui Shao, Linyu Shi, Shu Zhang, Wan-jin Chen, Ning Wang, Shiwen Wu, Hui Yang, Chunlong Xu, Guoling Li   +17 more
doaj   +2 more sources

Optimize nearly PAM-less adenine base editor to improve editing efficiency [PDF]

Jichu yixue yu linchuang, 2022
Objective To explore the effects of replacing different components of PAM-less adenine base editor (ABE) on its editing efficiency. Methods ABEmax was replaced with Tad-8e in SpRY-ABEmax to construct SpRY-ABE8e.
ZHOU Xiao-yu, WANG Xiao-yue
doaj   +1 more source

An adenine base editor with expanded targeting scope using SpCas9‐NGv1 in rice [PDF]

Plant Biotechnology Journal, 2019
Katsuya Negishi, Hidetaka Kaya, Kiyomi Abe   +2 more
exaly   +2 more sources

Development of TALE‐adenine base editors in plants

Plant Biotechnology Journal, 2023
SummaryBase editors enable precise nucleotide changes at targeted genomic loci without requiring double‐stranded DNA breaks or repair templates. TALE‐adenine base editors (TALE‐ABEs) are genome editing tools, composed of a DNA‐binding domain from transcription activator‐like effectors (TALEs), an engineered adenosine deaminase (TadA8e), and a cytosine ...
Dingbo Zhang, Jens Boch
openaire   +2 more sources

Base Editors for Citrus Gene Editing

Frontiers in Genome Editing, 2022
Base editors, such as adenine base editors (ABE) and cytosine base editors (CBE), provide alternatives for precise genome editing without generating double-strand breaks (DSBs), thus avoiding the risk of genome instability and unpredictable outcomes ...
Xiaoen Huang, Yuanchun Wang, Nian Wang
doaj   +1 more source

Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity. [PDF]

Nat Biotechnol, 2020
Applications of adenine base editors (ABEs) have been constrained by the limited compatibility of the deoxyadenosine deaminase component with Cas homologs other than SpCas9.
Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR.   +11 more
europepmc   +2 more sources

Porous Silicon Nanoneedles Efficiently Deliver Adenine Base Editor to Correct a Recurrent Pathogenic COL7A1 Variant in Recessive Dystrophic Epidermolysis Bullosa. [PDF]

Adv Mater
Base editing, a CRISPR-based genome editing technology, enables precise correction of single-nucleotide variants, promising resolutive treatment for monogenic genetic disorders like recessive dystrophic epidermolysis bullosa (RDEB).
Mustfa SA, Dimitrievska M, Wang C, Gu C, Sun N, Romańczuk K, Karpinski P, Łaczmański Ł, McGrath JA, Jacków-Malinowska J, Chiappini C.   +10 more
europepmc   +2 more sources

Human cell based directed evolution of adenine base editors with improved efficiency [PDF]

Nature Communications, 2021
Abstract Adenine base editors (ABE) are genome-editing tools that have been harnessed to introduce precise A•T to G•C conversion. However, the low activity of ABE at certain sites remains a major bottleneck that precludes efficacious applications.
Junhao Fu, Qing Li, Xiaoyu Liu, Tianxiang Tu, Xiujuan Lv, Xidi Yin, Jineng Lv, Zongming Song, Jia Qu, Jinwei Zhang, Jinsong Li, Feng Gu   +11 more
openaire   +3 more sources

Correction of DMD in human iPSC-derived cardiomyocytes by base-editing-induced exon skipping

Molecular Therapy: Methods & Clinical Development, 2023
Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene. Previously, we showed that adenine base editing (ABE) can efficiently correct a nonsense point mutation in a DMD mouse model.
Peipei Wang, Haiwen Li, Mandi Zhu, Rena Y. Han, Shuliang Guo, Renzhi Han   +5 more
doaj   +1 more source