Results 1 to 10 of about 515,050 (293)
CRISPR-Cas9 DNA Base-Editing and Prime-Editing [PDF]
International Journal of Molecular Sciences, 2020 Many genetic diseases and undesirable traits are due to base-pair alterations in genomic DNA. Base-editing, the newest evolution of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based technologies, can directly install point-mutations in cellular DNA without inducing a double-strand DNA break (DSB).
Michelle E Mcclements +2 more
exaly +4 more sources
Be-dataHIVE: a base editing database
BMC BioinformaticsBase editing is an enhanced gene editing approach that enables the precise transformation of single nucleotides and has the potential to cure rare diseases.
Lucas Schneider, Peter Minary
doaj +6 more sources
Base editing and prime editing in laboratory animals [PDF]
Laboratory Animals, 2021 Genome editing by programmable RNA-dependent Cas endonucleases has revolutionised the field of genome engineering, achieving targeted genomic change at unprecedented efficiencies with considerable application in laboratory animal research. Despite its ease of use and wide application, there remain concerns about the precision of this technology and a ...
Federico Caso, Benjamin Davies
core +6 more sources
Targeted RNA base editing for therapeutic: mechanisms and advances [PDF]
Pharmaceutical Science AdvancesRNA base editing, which enables RNA base modification through effector proteins guided by targeting systems, is a powerful technology to correct disease-associated point mutations.
Weikai Yan, Xiaocheng Weng
doaj +3 more sources
Target-AID-Mediated Multiplex Base Editing in Porcine Fibroblasts
Animals, 2021 Multiplex genome editing may induce genotoxicity and chromosomal rearrangements due to double-strand DNA breaks at multiple loci simultaneously induced by programmable nucleases, including CRISPR/Cas9. However, recently developed base-editing systems can
Soo-Young Yum, Goo Jang, Okjae Koo
doaj +2 more sources
HDAC inhibitors improve CRISPR-Cas9 mediated prime editing and base editing
Molecular Therapy: Nucleic Acids, 2022 Recent advances in CRISPR-Cas9 techniques, especially the discovery of base and prime editing, have significantly improved our ability to make precise changes in the genome.
Nan Liu +8 more
doaj +5 more sources
CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants
Synthetic and Systems Biotechnology, 2020 CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) has been extensively exploited as a genetic tool for genome editing.
Abdullah +5 more
doaj +2 more sources
Base Editing in Plants: Applications, Challenges, and Future Prospects
Frontiers in Plant Science, 2021 The ability to create targeted modifications in the genomes of plants using genome editing technologies has revolutionized research in crop improvement in the current dispensation of molecular biology.
Mawuli K. Azameti +2 more
doaj +2 more sources
Programmable DNA pyrimidine base editing via engineered uracil-DNA glycosylase
Nature CommunicationsDNA base editing technologies predominantly utilize engineered deaminases, limiting their ability to edit thymine and guanine directly. In this study, we successfully achieve base editing of both cytidine and thymine by leveraging the translesion DNA ...
Zongyi Yi +10 more
doaj +2 more sources
BMC Biology, 2018 Background Base Editing is a precise genome editing method that uses a deaminase-Cas9 fusion protein to mutate cytidine to thymidine in target DNA in situ without the generation of a double-strand break.
Matthew A. Coelho +8 more
doaj +2 more sources