Results 1 to 10 of about 25,918 (263)

CRISPR-CBEI: a Designing and Analyzing Tool Kit for Cytosine Base Editor-Mediated Gene Inactivation [PDF]

mSystems, 2020
Base editing is a promising technique, allowing precise single-base mutagenesis in genomes without double-strand DNA breaks or donor templates. Cytosine base editors (CBEs) convert cytosine to thymidine. In particular, CBEs can transform four codons, CAA,
Haopeng Yu, Zhaowei Wu, Xiangdan Chen, Quanjiang Ji, Shiheng Tao   +4 more
doaj   +4 more sources

CRISPR-mediated base editing in mice using cytosine deaminase base editor 4

Electronic Journal of Biotechnology, 2021
Background: Many human genetic diseases arise from point mutations. These genetic diseases can theoretically be corrected through gene therapy. However, gene therapy in clinical application is still far from mature.
Salah Adlat, Farooq Hayel, Ping Yang, Yang Chen, Zin Mar Oo, May Zun Zaw Myint, Rajiv Kumar Sah, Noor Bahadar, Mahmoud Al-Azab, Fatoumata Binta Bah, Yaowu Zheng, Xuechao Feng   +11 more
doaj   +4 more sources

Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells [PDF]

Genome Biology
Base editors are precise editing tools that employ deaminases to modify target DNA bases. The DYW-family of cytosine deaminases is structurally and phylogenetically distinct and might be harnessed for genome editing tools.
Dingbo Zhang, Fiona Parth, Laura Matos da Silva, Teng-Cheong Ha, Axel Schambach, Jens Boch   +5 more
doaj   +2 more sources

Engineering TadA ortholog-derived cytosine base editor without motif preference and adenosine activity limitation [PDF]

Nature Communications
The engineered TadA variants used in cytosine base editors (CBEs) present distinctive advantages, including a smaller size and fewer off-target effects compared to cytosine base editors that rely on natural deaminases.
Guoling Li, Xue Dong, Jiamin Luo, Tanglong Yuan, Tong Li, Guoli Zhao, Hainan Zhang, Jingxing Zhou, Zhenhai Zeng, Shuna Cui, Haoqiang Wang, Yin Wang, Yuyang Yu, Yuan Yuan, Erwei Zuo, Chunlong Xu, Jinhai Huang, Yingsi Zhou   +17 more
doaj   +2 more sources

Cytosine base editor-DNA binding domain fusions for editing window modulation in the RNP format [PDF]

BMC Biotechnology
Summary Base editing technologies allow for the precise and efficient installation of defined nucleotide substitutions into a target genome without the introduction of double strand breaks or DNA templates.
Erin Brettmann, Fuqiang Chen, Stephen Beishir, Graeme Garvey   +3 more
doaj   +2 more sources

Gene editing and scalable functional genomic screening in Leishmania species using the CRISPR/Cas9 cytosine base editor toolbox LeishBASEedit [PDF]

eLife, 2023
CRISPR/Cas9 gene editing has revolutionised loss-of-function experiments in Leishmania, the causative agent of leishmaniasis. As Leishmania lack a functional non-homologous DNA end joining pathway however, obtaining null mutants typically requires ...
Markus Engstler, Tom Beneke
doaj   +2 more sources

Cytosine Base Editor (hA3A-BE3-NG)-Mediated Multiple Gene Editing for Pyramid Breeding in Pigs [PDF]

Frontiers in Genetics, 2020
Pig is an important agricultural economic animal, providing large amount of meat products. With the development of functional genomics and bioinformatics, lots of genes and functional single nucleotide polymorphisms (SNPs) related to disease resistance ...
Yu Wang, Yu Wang, Dengfeng Bi, Dengfeng Bi, Guosong Qin, Ruigao Song, Ruigao Song, Jing Yao, Chunwei Cao, Chunwei Cao, Qiantao Zheng, Naipeng Hou, Naipeng Hou, Yanfang Wang, Yanfang Wang, Jianguo Zhao, Jianguo Zhao   +16 more
doaj   +2 more sources

A universal and wide-range cytosine base editor via domain-inlaid and fidelity-optimized CRISPR-FrCas9 [PDF]

Nature Communications
CRISPR-based base editor (BE) offer diverse editing options for genetic engineering of microorganisms, but its application is limited by protospacer adjacent motif (PAM) sequences, context preference, editing window, and off-target effects.
Lan Hu, Jing Han, Hao-Da Wang, Zhou-Hua Cheng, Chang-Ce Lv, Dong-Feng Liu, Han-Qing Yu   +6 more
doaj   +2 more sources

Highly efficient CRISPR-SaKKH tools for plant multiplex cytosine base editing

Crop Journal, 2020
Base editing, as an expanded clustered regularly interspaced short palindromic repeats (CRISPR)-Cas genome editing strategy, permits precise and irreversible nucleotide conversion. SaKKH, an efficient variant of a Cas9 ortholog from Staphylococcus aureus
Chengwei Zhang, Feipeng Wang, Si Zhao, Guiting Kang, Jinling Song, Lu Li, Jinxiao Yang   +6 more
doaj   +3 more sources

Engineered cytosine base editor enabling broad-scope and high-fidelity gene editing in Streptomyces [PDF]

Nature Communications
Base editing (BE) faces protospacer adjacent motif (PAM) constraints and off-target effects in both eukaryotes and prokaryotes. For Streptomyces, renowned as one of the most prolific bacterial producers of antibiotics, the challenges are more pronounced ...
Jian Wang, Ke Wang, Zhe Deng, Zhiyu Zhong, Guo Sun, Qing Mei, Fuling Zhou, Zixin Deng, Yuhui Sun   +8 more
doaj   +2 more sources