Results 1 to 10 of about 15,248 (235)

CRISPR-CBEI: a Designing and Analyzing Tool Kit for Cytosine Base Editor-Mediated Gene Inactivation [PDF]

mSystems, 2020
Base editing is a promising technique, allowing precise single-base mutagenesis in genomes without double-strand DNA breaks or donor templates. Cytosine base editors (CBEs) convert cytosine to thymidine. In particular, CBEs can transform four codons, CAA,
Haopeng Yu, Zhaowei Wu, Xiangdan Chen, Quanjiang Ji, Shiheng Tao   +4 more
doaj   +4 more sources

Cytosine base editors optimized for genome editing in potato protoplasts

Frontiers in Genome Editing, 2023
In this study, we generated and compared three cytidine base editors (CBEs) tailor-made for potato (Solanum tuberosum), which conferred up to 43% C-to-T conversion of all alleles in the protoplast pool.
Ida Westberg, Frida Meijer Carlsen, Ida Elisabeth Johansen, Bent Larsen Petersen   +3 more
doaj   +7 more sources

Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity. [PDF]

Nat Biotechnol, 2023
AbstractCytosine base editors (CBEs) are larger and can suffer from higher off-target activity or lower on-target editing efficiency than current adenine base editors (ABEs). To develop a CBE that retains the small size, low off-target activity and high on-target activity of current ABEs, we evolved the highly active deoxyadenosine deaminase TadA-8e to
Neugebauer ME, Hsu A, Arbab M, Krasnow NA, McElroy AN, Pandey S, Doman JL, Huang TP, Raguram A, Banskota S, Newby GA, Tolar J, Osborn MJ, Liu DR.   +13 more
europepmc   +3 more sources

Efficient CRISPR-Cas9 based cytosine base editors for phytopathogenic bacteria

Communications Biology, 2023
A versatile cytosine base editor system is developed to achieve highly efficient C to T conversion in the genome of a series of phytopathogenic bacteria, including Xanthomonas, Pseudomonas, Erwinia, and Agrobacterium.
Chenhao Li, Longfei Wang, Leland J. Cseke, Fernanda Vasconcelos, Jose Carlos Huguet-Tapia, Walter Gassmann, Laurens Pauwels, Frank F. White, Hansong Dong, Bing Yang   +9 more
doaj   +4 more sources

Engineering soybean with high levels of herbicide resistance with a Cas12‐SF01‐based cytosine base editor [PDF]

Plant Biotechnology Journal
Qingfeng Niu
exaly   +4 more sources

Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells [PDF]

Genome Biology
Base editors are precise editing tools that employ deaminases to modify target DNA bases. The DYW-family of cytosine deaminases is structurally and phylogenetically distinct and might be harnessed for genome editing tools.
Dingbo Zhang, Fiona Parth, Laura Matos da Silva, Teng-Cheong Ha, Axel Schambach, Jens Boch   +5 more
doaj   +2 more sources

Small-molecule compounds boost genome-editing efficiency of cytosine base editor. [PDF]

Nucleic Acids Res, 2021
Abstract Cytosine base editor (CBE) enables targeted C-to-T conversions at single base-pair resolution and thus has potential therapeutic applications in humans. However, the low efficiency of the system limits practical use of this approach.
Zhao T, Li Q, Zhou C, Lv X, Liu H, Tu T, Tang N, Cheng Y, Liu X, Liu C, Zhao J, Song Z, Wang H, Li J, Gu F, Gu F.   +15 more
europepmc   +4 more sources

Cytosine base editor 4 but not adenine base editor generates off-target mutations in mouse embryos. [PDF]

Commun Biol, 2020
AbstractDeaminase base editing has emerged as a tool to install or correct point mutations in the genomes of living cells in a wide range of organisms. However, the genome-wide off-target effects introduced by base editors in the mammalian genome have been examined in only one study.
Lee HK, Smith HE, Liu C, Willi M, Hennighausen L.   +4 more
europepmc   +4 more sources

CRISPR-mediated base editing in mice using cytosine deaminase base editor 4

Electronic Journal of Biotechnology, 2021
Background: Many human genetic diseases arise from point mutations. These genetic diseases can theoretically be corrected through gene therapy. However, gene therapy in clinical application is still far from mature.
Salah Adlat, Farooq Hayel, Ping Yang, Yang Chen, Zin Mar Oo, May Zun Zaw Myint, Rajiv Kumar Sah, Noor Bahadar, Mahmoud Al-Azab, Fatoumata Binta Bah, Yaowu Zheng, Xuechao Feng   +11 more
doaj   +3 more sources

Engineering TadA ortholog-derived cytosine base editor without motif preference and adenosine activity limitation [PDF]

Nature Communications
The engineered TadA variants used in cytosine base editors (CBEs) present distinctive advantages, including a smaller size and fewer off-target effects compared to cytosine base editors that rely on natural deaminases.
Guoling Li, Xue Dong, Jiamin Luo, Tanglong Yuan, Tong Li, Guoli Zhao, Hainan Zhang, Jingxing Zhou, Zhenhai Zeng, Shuna Cui, Haoqiang Wang, Yin Wang, Yuyang Yu, Yuan Yuan, Erwei Zuo, Chunlong Xu, Jinhai Huang, Yingsi Zhou   +17 more
doaj   +2 more sources