CRISPR-mediated base editing in mice using cytosine deaminase base editor 4
Electronic Journal of Biotechnology, 2021 Background: Many human genetic diseases arise from point mutations. These genetic diseases can theoretically be corrected through gene therapy. However, gene therapy in clinical application is still far from mature.
Salah Adlat +11 more
doaj +5 more sources
Efficient CRISPR-Cas9 based cytosine base editors for phytopathogenic bacteria
Communications Biology, 2023 A versatile cytosine base editor system is developed to achieve highly efficient C to T conversion in the genome of a series of phytopathogenic bacteria, including Xanthomonas, Pseudomonas, Erwinia, and Agrobacterium.
Chenhao Li +9 more
doaj +5 more sources
CRISPR-CBEI: a Designing and Analyzing Tool Kit for Cytosine Base Editor-Mediated Gene Inactivation [PDF]
mSystems, 2020 Base editing is a promising technique, allowing precise single-base mutagenesis in genomes without double-strand DNA breaks or donor templates. Cytosine base editors (CBEs) convert cytosine to thymidine. In particular, CBEs can transform four codons, CAA,
Haopeng Yu +4 more
doaj +4 more sources
Cytosine base editor-DNA binding domain fusions for editing window modulation in the RNP format [PDF]
BMC BiotechnologySummary Base editing technologies allow for the precise and efficient installation of defined nucleotide substitutions into a target genome without the introduction of double strand breaks or DNA templates.
Erin Brettmann +3 more
doaj +3 more sources
Gene editing and scalable functional genomic screening in Leishmania species using the CRISPR/Cas9 cytosine base editor toolbox LeishBASEedit [PDF]
eLife, 2023 CRISPR/Cas9 gene editing has revolutionised loss-of-function experiments in Leishmania, the causative agent of leishmaniasis. As Leishmania lack a functional non-homologous DNA end joining pathway however, obtaining null mutants typically requires ...
Markus Engstler, Tom Beneke
doaj +3 more sources
A universal and wide-range cytosine base editor via domain-inlaid and fidelity-optimized CRISPR-FrCas9 [PDF]
Nature CommunicationsCRISPR-based base editor (BE) offer diverse editing options for genetic engineering of microorganisms, but its application is limited by protospacer adjacent motif (PAM) sequences, context preference, editing window, and off-target effects.
Lan Hu +6 more
doaj +3 more sources
Cytosine base editors optimized for genome editing in potato protoplasts
Frontiers in Genome Editing, 2023 In this study, we generated and compared three cytidine base editors (CBEs) tailor-made for potato (Solanum tuberosum), which conferred up to 43% C-to-T conversion of all alleles in the protoplast pool.
Ida Westberg +3 more
doaj +6 more sources
Cytosine base editors (CBEs) for inducing targeted DNA base editing in Nicotiana benthamiana
BMC Plant Biology, 2023 Background The base editors can introduce point mutations accurately without causing double-stranded DNA breaks or requiring donor DNA templates. Previously, cytosine base editors (CBEs) containing different deaminases are reported for precise and ...
Juan Luo +6 more
doaj +5 more sources
Small-molecule compounds boost genome-editing efficiency of cytosine base editor [PDF]
Nucleic Acids Research, 2021 Abstract Cytosine base editor (CBE) enables targeted C-to-T conversions at single base-pair resolution and thus has potential therapeutic applications in humans. However, the low efficiency of the system limits practical use of this approach.
Qing Li, Xiujuan Lv, Changbao Liu
exaly +5 more sources
Engineering soybean with high levels of herbicide resistance with a Cas12‐SF01‐based cytosine base editor [PDF]
Plant Biotechnology JournalQingfeng Niu +2 more
exaly +4 more sources