Screening of CRISPR/Cas base editors to target the AMD high-risk Y402H complement factor H variant [PDF]
Molecular Vision, 2019 Purpose: To evaluate the efficacy of using a CRISPR/Cas-mediated strategy to correct a common high-risk allele that is associated with age-related macular degeneration (AMD; rs1061170; NM_000186.3:c.1204T>C; NP_000177.2:p.His402Tyr) in the complement ...
Minh Thuan Nguyen Tran +9 more
doaj +3 more sources
Engineered cytosine base editor enabling broad-scope and high-fidelity gene editing in Streptomyces [PDF]
Nature CommunicationsBase editing (BE) faces protospacer adjacent motif (PAM) constraints and off-target effects in both eukaryotes and prokaryotes. For Streptomyces, renowned as one of the most prolific bacterial producers of antibiotics, the challenges are more pronounced ...
Jian Wang +8 more
doaj +2 more sources
Improved base editing and functional screening in <i>Leishmania</i> via co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, and a cytosine base editor. [PDF]
ElifeThe ability to analyze the function of all genes in a genome is highly desirable, yet challenging in Leishmania due to a repetitive genome, limited DNA repair mechanisms, and lack of RNA interference in most species.
Herrmann May N +6 more
europepmc +2 more sources
Microbiology Spectrum, 2022 Genome editing technology is a powerful tool for programming microbial cell factories. However, rat APOBEC1-derived cytosine base editor (CBE) that converts C•G to T•A at target genes induced DNA off-targets, regardless of single-guide RNA (sgRNA ...
Yu Been Heo +4 more
doaj +3 more sources
Eliminating predictable DNA off-target effects of cytosine base editor by using dual guiders including sgRNA and TALE [PDF]
Molecular Therapy, 2022 Shixue Gou, Yang Yang, Liangxue Lai
exaly +2 more sources
Aquaculture Reports, 2022 Economic crustaceans play important roles in aquaculture and create great benefits for the world agricultural economy. Studies have found that there are many single nucleotide polymorphism (SNP) sites in the crustacean genomes, which closely related to a
Yujie Liu +5 more
doaj +2 more sources
Enhancing genome editing in hPSCs through dual inhibition of DNA damage response and repair pathways
Nature CommunicationsPrecise genome editing is crucial for establishing isogenic human disease models and ex vivo stem cell therapy from the patient-derived hPSCs. Unlike Cas9-mediated knock-in, cytosine base editor and prime editor achieve the desirable gene correction ...
Ju-Chan Park +5 more
doaj +2 more sources
Single‐base editing of rs12603332 on chromosome 17q21 with a cytosine base editor regulates ORMDL3 and ATF6α expression [PDF]
Allergy: European Journal of Allergy and Clinical Immunology, 2022 Marina Miller +2 more
exaly +2 more sources
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity. [PDF]
Nat Biotechnol, 2023 AbstractCytosine base editors (CBEs) are larger and can suffer from higher off-target activity or lower on-target editing efficiency than current adenine base editors (ABEs). To develop a CBE that retains the small size, low off-target activity and high on-target activity of current ABEs, we evolved the highly active deoxyadenosine deaminase TadA-8e to
Neugebauer ME +13 more
europepmc +3 more sources
Analysis of Cytosine Base Editors in Bovine Zygotes: Efficiency and Editing Window Characterization Through Targeting the MYO7A Gene [PDF]
Current Issues in Molecular BiologyCytosine base editors (CBEs) enable precise C-to-T (G-to-A) conversions in genomic DNA, offering significant potential for specific gene editing. This study compared the prototypical Base Editor 3 (BE3) and a modified variant, BE3-Y130F, which utilizes ...
Junghyun Ryu +3 more
doaj +2 more sources