Results 21 to 30 of about 15,248 (235)

A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing

Nature Biotechnology, 2020
Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR-Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits.
Jae Joung, Julian Grunewald, Sowmya Iyer   +2 more
exaly   +6 more sources

High-Fidelity Cytosine Base Editing in a GC-Rich Corynebacterium glutamicum with Reduced DNA Off-Target Editing Effects

Microbiology Spectrum, 2022
Genome editing technology is a powerful tool for programming microbial cell factories. However, rat APOBEC1-derived cytosine base editor (CBE) that converts C•G to T•A at target genes induced DNA off-targets, regardless of single-guide RNA (sgRNA ...
Yu Been Heo, Gue-Ho Hwang, Seok Won Kang, Sangsu Bae, Han Min Woo   +4 more
doaj   +3 more sources

Research progress of base editing and prime editing tools based on the CRISPR/Cas system [PDF]

Molecular Therapy: Nucleic Acids
The base editor (BE) and prime editing guide RNA (pegRNA)-based prime editor (PE) technologies relying on the CRISPR/Cas system are very efficient gene editors that have been developed in recent years.
Herui Gao, Song Gao, Guoshi Kan, Leonid N. Valentovich, Yingfeng An   +4 more
doaj   +2 more sources

Eliminating predictable DNA off-target effects of cytosine base editor by using dual guiders including sgRNA and TALE [PDF]

Molecular Therapy, 2022
Liangxue Lai, Yang Yang, Shixue Gou
exaly   +2 more sources

Single‐base editing of rs12603332 on chromosome 17q21 with a cytosine base editor regulates ORMDL3 and ATF6α expression [PDF]

Allergy: European Journal of Allergy and Clinical Immunology, 2022
Alexis Komor, David Broide
exaly   +2 more sources

Analysis of Cytosine Base Editors in Bovine Zygotes: Efficiency and Editing Window Characterization Through Targeting the MYO7A Gene [PDF]

Current Issues in Molecular Biology
Cytosine base editors (CBEs) enable precise C-to-T (G-to-A) conversions in genomic DNA, offering significant potential for specific gene editing. This study compared the prototypical Base Editor 3 (BE3) and a modified variant, BE3-Y130F, which utilizes ...
Junghyun Ryu, Rebecca Tippner-Hedges, Martha Neuringer, Jon D. Hennebold   +3 more
doaj   +2 more sources

Adenine base editor engineering reduces editing of bystander cytosines [PDF]

Nature Biotechnology, 2021
Adenine base editors (ABEs) catalyze specific A-to-G conversions at genomic sites of interest. However, ABEs also induce cytosine deamination at the target site. To reduce the cytosine editing activity, we engineered a commonly used adenosine deaminase, TadA7.10, and found that ABE7.10 with a D108Q mutation in TadA7.10 exhibited tenfold reduced ...
You Kyeong Jeong, SeokHoon Lee, Gue-Ho Hwang, Sung-Ah Hong, Se-eun Park, Jin-Soo Kim, Jae-Sung Woo, Sangsu Bae   +7 more
openaire   +3 more sources

Development of an Efficient C-to-T Base-Editing System and Its Application to Cellulase Transcription Factor Precise Engineering in Thermophilic Fungus Myceliophthora thermophila

Microbiology Spectrum, 2022
Myceliophthora thermophila is a thermophilic fungus with great potential in biorefineries and biotechnology. The base editor is an upgraded version of the clustered regularly interspaced short palindromic repeats (CRISPR)-dependent genome-editing tool ...
Chenyang Zhang, Nan Li, Lang Rao, Jingen Li, Qian Liu, Chaoguang Tian   +5 more
doaj   +1 more source

Base Editors for Citrus Gene Editing

Frontiers in Genome Editing, 2022
Base editors, such as adenine base editors (ABE) and cytosine base editors (CBE), provide alternatives for precise genome editing without generating double-strand breaks (DSBs), thus avoiding the risk of genome instability and unpredictable outcomes ...
Xiaoen Huang, Yuanchun Wang, Nian Wang
doaj   +1 more source

Developing high-efficiency base editors by combining optimized synergistic core components with new types of nuclear localization signal peptide

Crop Journal, 2020
The clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein (Cas) system has been widely used for genome editing. In this system, the cytosine base editor (CBE) and adenine base editor (ABE) allow generating precise ...
Feipeng Wang, Chengwei Zhang, Wen Xu, Shuang Yuan, Jinling Song, Lu Li, Jiuran Zhao, Jinxiao Yang   +7 more
doaj   +1 more source