Results 21 to 30 of about 25,918 (263)

Single‐base editing of rs12603332 on chromosome 17q21 with a cytosine base editor regulates ORMDL3 and ATF6α expression [PDF]

Allergy: European Journal of Allergy and Clinical Immunology, 2022
Alexis C Komor, David H Broide
exaly   +2 more sources

Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity. [PDF]

Nat Biotechnol, 2023
AbstractCytosine base editors (CBEs) are larger and can suffer from higher off-target activity or lower on-target editing efficiency than current adenine base editors (ABEs). To develop a CBE that retains the small size, low off-target activity and high on-target activity of current ABEs, we evolved the highly active deoxyadenosine deaminase TadA-8e to
Neugebauer ME, Hsu A, Arbab M, Krasnow NA, McElroy AN, Pandey S, Doman JL, Huang TP, Raguram A, Banskota S, Newby GA, Tolar J, Osborn MJ, Liu DR.   +13 more
europepmc   +3 more sources

Analysis of Cytosine Base Editors in Bovine Zygotes: Efficiency and Editing Window Characterization Through Targeting the MYO7A Gene [PDF]

Current Issues in Molecular Biology
Cytosine base editors (CBEs) enable precise C-to-T (G-to-A) conversions in genomic DNA, offering significant potential for specific gene editing. This study compared the prototypical Base Editor 3 (BE3) and a modified variant, BE3-Y130F, which utilizes ...
Junghyun Ryu, Rebecca Tippner-Hedges, Martha Neuringer, Jon D. Hennebold   +3 more
doaj   +2 more sources

A cytosine base editor toolkit with varying activity windows and target scopes for versatile gene manipulation in plants [PDF]

Nucleic Acids Research, 2022
Xiangyu Xiong, ZhenXiang Li, Chenlong Li   +2 more
exaly   +2 more sources

Cytosine base editors with increased PAM and deaminase motif flexibility for gene editing in zebrafish

Nature Communications
Cytosine base editing is a powerful tool for making precise single nucleotide changes in cells and model organisms like zebrafish, which are valuable for studying human diseases.
Yu Zhang, Yang Liu, Wei Qin, Shaohui Zheng, Jiawang Xiao, Xinxin Xia, Xuanyao Yuan, Jingjing Zeng, Yu Shi, Yan Zhang, Hui Ma, Gaurav K. Varshney, Ji-Feng Fei, Yanmei Liu   +13 more
doaj   +3 more sources

Cytosine base editor 4 but not adenine base editor generates off-target mutations in mouse embryos. [PDF]

Commun Biol, 2020
AbstractDeaminase base editing has emerged as a tool to install or correct point mutations in the genomes of living cells in a wide range of organisms. However, the genome-wide off-target effects introduced by base editors in the mammalian genome have been examined in only one study.
Lee HK, Smith HE, Liu C, Willi M, Hennighausen L.   +4 more
europepmc   +4 more sources

Adenine base editor engineering reduces editing of bystander cytosines [PDF]

Nature Biotechnology, 2021
Adenine base editors (ABEs) catalyze specific A-to-G conversions at genomic sites of interest. However, ABEs also induce cytosine deamination at the target site. To reduce the cytosine editing activity, we engineered a commonly used adenosine deaminase, TadA7.10, and found that ABE7.10 with a D108Q mutation in TadA7.10 exhibited tenfold reduced ...
You Kyeong Jeong, SeokHoon Lee, Gue-Ho Hwang, Sung-Ah Hong, Se-eun Park, Jin-Soo Kim, Jae-Sung Woo, Sangsu Bae   +7 more
openaire   +3 more sources

Cytosine-to-Uracil Deamination by SssI DNA Methyltransferase [PDF]

, 2013
The prokaryotic DNA(cytosine-5)methyltransferase M.SssI shares the specificity of eukaryotic DNA methyltransferases (CG) and is an important model and experimental tool in the study of eukaryotic DNA methylation.
A Beletskii, A Jeltsch, AN Sharath, Antal Kiss, AS Yang, B Bandaru, B Bandaru, B Chaikind, BM Hall, BTF van der Gun, DM Woodcock, EA Raleigh, EU Selker, EV Koudan, G Pósfai, GL Xu, H Gowher, I Stier, Ildikó Stier, J Sambrook, JC Shen, JC Shen, JC Shen, JM Zingg, JM Zingg, KM Reinisch, LA Frederico, LM Guzman, M Frommer, M Wyszynski, Martin G. Marinus, MH Sibley, P Rathert, P Renbaum, PA Waite-Rees, PM Warnecke, R Métivier, R Shapiro, RJ Roberts, RY-H Wang, S Kumar, SE Luria, T Durfee, TA Kunkel, UT Sankpal, WA Rosche, X Cheng, X Zhang   +47 more
core   +14 more sources

Development of an Efficient C-to-T Base-Editing System and Its Application to Cellulase Transcription Factor Precise Engineering in Thermophilic Fungus Myceliophthora thermophila

Microbiology Spectrum, 2022
Myceliophthora thermophila is a thermophilic fungus with great potential in biorefineries and biotechnology. The base editor is an upgraded version of the clustered regularly interspaced short palindromic repeats (CRISPR)-dependent genome-editing tool ...
Chenyang Zhang, Nan Li, Lang Rao, Jingen Li, Qian Liu, Chaoguang Tian   +5 more
doaj   +1 more source

Base Editors for Citrus Gene Editing

Frontiers in Genome Editing, 2022
Base editors, such as adenine base editors (ABE) and cytosine base editors (CBE), provide alternatives for precise genome editing without generating double-strand breaks (DSBs), thus avoiding the risk of genome instability and unpredictable outcomes ...
Xiaoen Huang, Yuanchun Wang, Nian Wang
doaj   +1 more source