Results 41 to 50 of about 15,403 (255)
Crop Journal, 2020 The clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein (Cas) system has been widely used for genome editing. In this system, the cytosine base editor (CBE) and adenine base editor (ABE) allow generating precise ...
Feipeng Wang +7 more
doaj +1 more source
Improved cytosine base editors generated from TadA variants
Nature Biotechnology, 2023 AbstractCytosine base editors (CBEs) enable programmable genomic C·G-to-T·A transition mutations and typically comprise a modified CRISPR–Cas enzyme, a naturally occurring cytidine deaminase, and an inhibitor of uracil repair. Previous studies have shown that CBEs utilizing naturally occurring cytidine deaminases may cause unguided, genome-wide ...
Lam, Dieter K. +15 more
openaire +3 more sources
Targeting fidelity of adenine and cytosine base editors in mouse embryos [PDF]
Nature Communications, 2018 AbstractBase editing directly converts a target base pair into a different base pair in the genome of living cells without introducing double-stranded DNA breaks. While cytosine base editors (CBE) and adenine base editors (ABE) are used to install and correct point mutations in a wide range of organisms, the extent and distribution of off-target edits ...
Hye Kyung Lee +6 more
openaire +3 more sources
Examination of the Cell Cycle Dependence of Cytosine and Adenine Base Editors
Frontiers in Genome Editing, 2022 Base editors (BEs) are genome editing agents that install point mutations with high efficiency and specificity. Due to their reliance on uracil and inosine DNA damage intermediates (rather than double-strand DNA breaks, or DSBs), it has been hypothesized that BEs rely on more ubiquitous DNA repair pathways than DSB-reliant genome editing methods, which
Cameron A. Burnett +6 more
openaire +5 more sources
One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids [PDF]
, 2023 Optimization of CRISPR/Cas9-mediated genome engineering has resulted in base editors that hold promise for mutation repair and disease modeling. Here, we demonstrate the application of base editors for the generation of complex tumor models in human ASC ...
Gijs van Son +16 more
core +2 more sources
CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants
Synthetic and Systems Biotechnology, 2020 CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) has been extensively exploited as a genetic tool for genome editing.
Abdullah +5 more
doaj +1 more source
Development of a Simple and Quick Method to Assess Base Editing in Human Cells
Molecular Therapy: Nucleic Acids, 2020 Base editing is a form of genome editing that can directly convert a single base (C or A) to another base (T or G), which is of great potential in biomedical applications.
Xiujuan Lv +12 more
doaj +1 more source
In vivo mitochondrial base editing via adeno-associated viral delivery to mouse post-mitotic tissue
Nature Communications, 2022 Mutations in mitochondrial DNA can lead to clinically heterogeneous disease. Here the authors demonstrate in vivo base editing of mouse mitochondrial DNA in a post-mitotic tissue by AAV delivery of DddA-derived cytosine base editor (DdCBE).
Pedro Silva-Pinheiro +5 more
doaj +1 more source
Frontiers in Plant Science, 2021 The demand for vegetable oil, which is mainly used for dietary purposes and cooking, is steadily increasing worldwide. It is often desirable to reduce unsaturation levels of fatty acids in order to increase storage stability and reduce trans-fat ...
Mid-Eum Park +3 more
doaj +1 more source
Optimization of the base editor BE4max in chicken somatic cells
Poultry Science, 2022 : Advanced animal reproductive and breeding biotechnology has made it possible to alter traits or create new genetic resources by the direct knock-in or knock-out of target genes.
Tianpeng Xu +8 more
doaj +1 more source