Results 41 to 50 of about 15,248 (235)

TadA orthologs enable both cytosine and adenine editing of base editors

Nature Communications, 2023
AbstractCytidine and adenosine deaminases are required for cytosine and adenine editing of base editors respectively, and no single deaminase could enable concurrent and comparable cytosine and adenine editing. Additionally, distinct properties of cytidine and adenosine deaminases lead to various types of off-target effects, including Cas9-indendepent ...
Shuqian Zhang, Bo Yuan, Jixin Cao, Liting Song, Jinlong Chen, Jiayi Qiu, Zilong Qiu, Xing-Ming Zhao, Jingqi Chen, Tian-Lin Cheng   +9 more
openaire   +3 more sources

Improved plant cytosine base editors with high editing activity, purity, and specificity [PDF]

Plant Biotechnology Journal, 2021
SummaryCytosine base editors (CBEs) are great additions to the expanding genome editing toolbox. To improve C‐to‐T base editing in plants, we first compared seven cytidine deaminases in the BE3‐like configuration in rice. We found A3A/Y130F‐CBE_V01 resulted in the highest C‐to‐T base editing efficiency in both rice and Arabidopsis.
Qiurong Ren, Simon Sretenovic, Guanqing Liu, Zhaohui Zhong, Jiaheng Wang, Lan Huang, Xu Tang, Yachong Guo, Li Liu, Yuechao Wu, Jie Zhou, Yuxin Zhao, Han Yang, Yao He, Shishi Liu, Desuo Yin, Rocio Mayorga, Xuelian Zheng, Tao Zhang, Yiping Qi, Yong Zhang   +20 more
openaire   +2 more sources

HDAC inhibitors improve CRISPR-Cas9 mediated prime editing and base editing

Molecular Therapy: Nucleic Acids, 2022
Recent advances in CRISPR-Cas9 techniques, especially the discovery of base and prime editing, have significantly improved our ability to make precise changes in the genome.
Nan Liu, Lifang Zhou, Guifeng Lin, Yun Hu, Yaoge Jiao, Yanhong Wang, Jingming Liu, Shengyong Yang, Shaohua Yao   +8 more
doaj   +1 more source

Cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing

Communications Biology, 2021
Jiao Fan, Yige Ding, et al. examine the impact of cytosine and adenine deaminases on transcriptome-wide gene expression and splicing in HeLa and HEK293T cells.
Jiao Fan, Yige Ding, Chao Ren, Ziguo Song, Jie Yuan, Qiuzhen Chen, Chenchen Du, Chao Li, Xiaolong Wang, Wenjie Shu   +9 more
doaj   +1 more source

Two Compact Cas9 Ortholog-Based Cytosine Base Editors Expand the DNA Targeting Scope and Applications In Vitro and In Vivo

Frontiers in Cell and Developmental Biology, 2022
CRISPR/Cas9-based base editing tools enable precise genomic installation and hold great promise for gene therapy, whereas the big size of Cas9 nucleases and its reliability on specific protospacer adjacent motif (PAM) sequences as well as target site ...
Susu Wu, Liping Li, Min Li, Shiyu Sun, Shiyu Sun, Yuting Zhao, Xiaowen Xue, Feiyu Chen, Jingli Zhong, Junfan Guo, Junfan Guo, Qianhui Qu, Xiongjun Wang, Zhen Liu, Zhen Liu, Yunbo Qiao   +15 more
doaj   +1 more source

Genome Engineering in Plant Using an Efficient CRISPR-xCas9 Toolset With an Expanded PAM Compatibility

Frontiers in Genome Editing, 2020
The CRISPR-Cas9 system enables simple, rapid, and effective genome editing in many species. Nevertheless, the requirement of an NGG protospacer adjacent motif (PAM) for the widely used canonical Streptococcus pyogenes Cas9 (SpCas9) limits the potential ...
Chengwei Zhang, Guiting Kang, Xinxiang Liu, Si Zhao, Shuang Yuan, Lu Li, Yongxing Yang, Feipeng Wang, Xiang Zhang, Jinxiao Yang   +9 more
doaj   +1 more source

Precise genome editing with base editors

Medical Review, 2023
Single-nucleotide variants account for about half of known pathogenic genetic variants in human. Genome editing strategies by reversing pathogenic point mutations with minimum side effects have great therapeutic potential and are now being actively ...
Liu Hongcai, Zhu Yao, Li Minjie, Gu Zhimin   +3 more
doaj   +1 more source

Cytosine but not adenine base editor generates mutations in mice [PDF]

, 2019
ABSTRACT Deaminase base editing has emerged as a tool to install or correct point mutations in the genomes of living cells in a wide range of organisms and its ultimate success therapeutically depends on its accuracy. Here we have investigated the fidelity of cytosine base editor 4 (BE4) and adenine base editor (ABE) in mouse embryos ...
Lee, Hye Kyung, Smith, Harold E., Liu, Chengyu, Willi, Michaela, Hennighausen, Lothar   +4 more
openaire   +1 more source

Adeno‐associated virus serotype 2 capsid variants for improved liver‐directed gene therapy

Hepatology, EarlyView., 2022
Abstract Background and Aims Current liver‐directed gene therapies look for adeno‐associated virus (AAV) vectors with improved efficacy. With this background, capsid engineering is explored. Whereas shuffled capsid library screenings have resulted in potent liver targeting variants with one first vector in human clinical trials, modifying natural ...
Nadja Meumann, Marti Cabanes‐Creus, Moritz Ertelt, Renina Gale Navarro, Julie Lucifora, Qinggong Yuan, Karin Nien‐Huber, Ahmed Abdelrahman, Xuan‐Khang Vu, Liang Zhang, Ann‐Christin Franke, Christian Schmithals, Albrecht Piiper, Annabelle Vogt, Maria Gonzalez‐Carmona, Jochen T. Frueh, Evelyn Ullrich, Philip Meuleman, Steven R. Talbot, Margarete Odenthal, Michael Ott, Erhard Seifried, Clara T. Schoeder, Joachim Schwäble, Leszek Lisowski, Hildegard Büning   +25 more
wiley   +1 more source

Engineering of cytosine base editors with DNA damage minimization and editing scope diversification

Nucleic Acids Research, 2023
Abstract Cytosine base editors (CBEs), which enable precise C-to-T substitutions, have been restricted by potential safety risks, including DNA off-target edits, RNA off-target edits and additional genotoxicity such as DNA damages induced by double-strand breaks (DSBs).
Bo Yuan, Shuqian Zhang, Liting Song, Jinlong Chen, Jixin Cao, Jiayi Qiu, Zilong Qiu, Jingqi Chen, Xing-Ming Zhao, Tian-Lin Cheng   +9 more
openaire   +2 more sources