Results 41 to 50 of about 25,918 (263)
In vivo mitochondrial base editing via adeno-associated viral delivery to mouse post-mitotic tissue
Nature Communications, 2022 Mutations in mitochondrial DNA can lead to clinically heterogeneous disease. Here the authors demonstrate in vivo base editing of mouse mitochondrial DNA in a post-mitotic tissue by AAV delivery of DddA-derived cytosine base editor (DdCBE).
Pedro Silva-Pinheiro +5 more
doaj +1 more source
Non-mammalian model organisms in epigenetic research : an overview [PDF]
, 2020 Recent advances in sequencing technology and genome editing tools had an indisputably enormous impact on our understanding of complex biological pathways and their genetic and epigenetic regulation.
Baruah, Sri Kartik +3 more
core +2 more sources
Optimization of the base editor BE4max in chicken somatic cells
Poultry Science, 2022 : Advanced animal reproductive and breeding biotechnology has made it possible to alter traits or create new genetic resources by the direct knock-in or knock-out of target genes.
Tianpeng Xu +8 more
doaj +1 more source
Frontiers in Plant Science, 2021 The demand for vegetable oil, which is mainly used for dietary purposes and cooking, is steadily increasing worldwide. It is often desirable to reduce unsaturation levels of fatty acids in order to increase storage stability and reduce trans-fat ...
Mid-Eum Park +3 more
doaj +1 more source
Improved plant cytosine base editors with high editing activity, purity, and specificity [PDF]
Plant Biotechnology Journal, 2021 SummaryCytosine base editors (CBEs) are great additions to the expanding genome editing toolbox. To improve C‐to‐T base editing in plants, we first compared seven cytidine deaminases in the BE3‐like configuration in rice. We found A3A/Y130F‐CBE_V01 resulted in the highest C‐to‐T base editing efficiency in both rice and Arabidopsis.
Qiurong Ren +20 more
openaire +2 more sources
Sequence-specific prediction of the efficiencies of adenine and cytosine base editors
Nature Biotechnology, 2020 Base editors, including adenine base editors (ABEs)1 and cytosine base editors (CBEs)2,3, are widely used to induce point mutations. However, determining whether a specific nucleotide in its genomic context can be edited requires time-consuming experiments.
Myungjae Song +14 more
openaire +3 more sources
TadA orthologs enable both cytosine and adenine editing of base editors
Nature Communications, 2023 AbstractCytidine and adenosine deaminases are required for cytosine and adenine editing of base editors respectively, and no single deaminase could enable concurrent and comparable cytosine and adenine editing. Additionally, distinct properties of cytidine and adenosine deaminases lead to various types of off-target effects, including Cas9-indendepent ...
Shuqian Zhang +9 more
openaire +3 more sources
Gene body methylation patterns in Daphnia are associated with gene family size [PDF]
, 2016 The relation between gene body methylation and gene function remains elusive. Yet, our understanding of this relationship can contribute significant knowledge on how and why organisms target specific gene bodies for methylation.
Asselman, Jana +3 more
core +2 more sources
TET2 inhibits differentiation of embryonic stem cells but does not overcome methylation-induced gene silencing [PDF]
, 2014 TET2 is a methylcytosine dioxygenase that is frequently mutated in myeloid malignancies, notably myelodysplasia and acute myeloid leukemia. TET2 catalyses the conversion of 5′-methylcytosine to 5′-hydroxymethylcytosine within DNA and has been implicated ...
Chevassut, Timothy +2 more
core +3 more sources
HDAC inhibitors improve CRISPR-Cas9 mediated prime editing and base editing
Molecular Therapy: Nucleic Acids, 2022 Recent advances in CRISPR-Cas9 techniques, especially the discovery of base and prime editing, have significantly improved our ability to make precise changes in the genome.
Nan Liu +8 more
doaj +1 more source