Results 51 to 60 of about 15,403 (255)

Targeted Mutagenesis in Mice Using a Base Editor

, 2023
Base editors, such as cytosine and adenine base editors, are composed of nickase Cas9 (nCas9) and deaminase and serve as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based enzymatic tools for specific nucleotide substitutions.
Jeong, Tae Yeong, Kim, Kyoungmi, Lim, Soo-Yeon, Seong, Je Kyung   +3 more
core   +1 more source

Sequence-specific prediction of the efficiencies of adenine and cytosine base editors

Nature Biotechnology, 2020
Base editors, including adenine base editors (ABEs)1 and cytosine base editors (CBEs)2,3, are widely used to induce point mutations. However, determining whether a specific nucleotide in its genomic context can be edited requires time-consuming experiments.
Myungjae Song, Hui Kwon Kim, Sungtae Lee, Younggwang Kim, Sang-Yeon Seo, Jinman Park, Jae Woo Choi, Hyewon Jang, Jeong Hong Shin, Seonwoo Min, Zhejiu Quan, Ji Hun Kim, Hoon Chul Kang, Sungroh Yoon, Hyongbum Henry Kim   +14 more
openaire   +3 more sources

Improved plant cytosine base editors with high editing activity, purity, and specificity [PDF]

Plant Biotechnology Journal, 2021
SummaryCytosine base editors (CBEs) are great additions to the expanding genome editing toolbox. To improve C‐to‐T base editing in plants, we first compared seven cytidine deaminases in the BE3‐like configuration in rice. We found A3A/Y130F‐CBE_V01 resulted in the highest C‐to‐T base editing efficiency in both rice and Arabidopsis.
Qiurong Ren, Simon Sretenovic, Guanqing Liu, Zhaohui Zhong, Jiaheng Wang, Lan Huang, Xu Tang, Yachong Guo, Li Liu, Yuechao Wu, Jie Zhou, Yuxin Zhao, Han Yang, Yao He, Shishi Liu, Desuo Yin, Rocio Mayorga, Xuelian Zheng, Tao Zhang, Yiping Qi, Yong Zhang   +20 more
openaire   +2 more sources

TadA orthologs enable both cytosine and adenine editing of base editors

Nature Communications, 2023
AbstractCytidine and adenosine deaminases are required for cytosine and adenine editing of base editors respectively, and no single deaminase could enable concurrent and comparable cytosine and adenine editing. Additionally, distinct properties of cytidine and adenosine deaminases lead to various types of off-target effects, including Cas9-indendepent ...
Shuqian Zhang, Bo Yuan, Jixin Cao, Liting Song, Jinlong Chen, Jiayi Qiu, Zilong Qiu, Xing-Ming Zhao, Jingqi Chen, Tian-Lin Cheng   +9 more
openaire   +3 more sources

Generation of JAG1 gene c.1615C > T heterozygous mutation human embryonic stem cell line (SDQLCHe001-A) using cytosine base editor

, 2023
Pathogenic variants in Jagged-1 (JAG1), which encodes the ligand of the Notch receptor, had been demonstrated to cause Alagille syndrome. However, there is no evidence to support any genotype-phenotype correlations. Here, we generated a gene-edited human
Bin Wang, Zirui Wan, Haiyan Zhang, Xiaohua Jin, Yue Li, Xu Ma, Jingyun Guan, Yi Liu, Xiaomeng Yang   +8 more
core   +1 more source

CRISPR-Cas9-Mediated Cytosine Base Editing Screen for the Functional Assessment of CALR Intron Variants in Japanese Encephalitis Virus Replication

, 2023
The Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes viral encephalitis in humans, pigs and other mammals across Asia and the Western Pacific.
Xiaosong Han, Yinyu Su, Bingrong Xu, Xinyun Li, Xinyi Wang, Changzhi Zhao, Shengsong Xie, Sheng Li, Youcai Xiong, Xiaoning Xi, Jinxue Ruan, Liangliang Fu, Hailong Liu, Ruigao He, Chong Xiong, Yue Xiang   +15 more
core   +1 more source

HDAC inhibitors improve CRISPR-Cas9 mediated prime editing and base editing

Molecular Therapy: Nucleic Acids, 2022
Recent advances in CRISPR-Cas9 techniques, especially the discovery of base and prime editing, have significantly improved our ability to make precise changes in the genome.
Nan Liu, Lifang Zhou, Guifeng Lin, Yun Hu, Yaoge Jiao, Yanhong Wang, Jingming Liu, Shengyong Yang, Shaohua Yao   +8 more
doaj   +1 more source

High-Efficiency Multiplexed Cytosine Base Editors for Natural Product Synthesis in Yarrowia lipolytica

, 2023
Yarrowia lipolytica is an industrial host with a high fatty acid flux. Even though CRISPR-based tools have accelerated its metabolic engineering, there remains a need to develop tools for rapid multiplexed strain engineering to accelerate the design ...
Dheeraj Pedada (17060477), Mark Blenner, Anthony Stohr, Lummy Monteiro (17060474), Vijaydev Ganesan (17060471), Dheeraj Pedada, Mark Blenner (1376295), Vijaydev Ganesan, Anthony Stohr (17060480), Lummy Monteiro   +9 more
core   +1 more source

Cytosine but not adenine base editor generates mutations in mice [PDF]

, 2019
ABSTRACT Deaminase base editing has emerged as a tool to install or correct point mutations in the genomes of living cells in a wide range of organisms and its ultimate success therapeutically depends on its accuracy. Here we have investigated the fidelity of cytosine base editor 4 (BE4) and adenine base editor (ABE) in mouse embryos ...
Lee, Hye Kyung, Smith, Harold E., Liu, Chengyu, Willi, Michaela, Hennighausen, Lothar   +4 more
openaire   +1 more source

Cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing

Communications Biology, 2021
Jiao Fan, Yige Ding, et al. examine the impact of cytosine and adenine deaminases on transcriptome-wide gene expression and splicing in HeLa and HEK293T cells.
Jiao Fan, Yige Ding, Chao Ren, Ziguo Song, Jie Yuan, Qiuzhen Chen, Chenchen Du, Chao Li, Xiaolong Wang, Wenjie Shu   +9 more
doaj   +1 more source