Results 61 to 70 of about 25,918 (263)

Engineering of cytosine base editors with DNA damage minimization and editing scope diversification

Nucleic Acids Research, 2023
Abstract Cytosine base editors (CBEs), which enable precise C-to-T substitutions, have been restricted by potential safety risks, including DNA off-target edits, RNA off-target edits and additional genotoxicity such as DNA damages induced by double-strand breaks (DSBs).
Bo Yuan, Shuqian Zhang, Liting Song, Jinlong Chen, Jixin Cao, Jiayi Qiu, Zilong Qiu, Jingqi Chen, Xing-Ming Zhao, Tian-Lin Cheng   +9 more
openaire   +2 more sources

Genome- and transcriptome-wide off-target analyses of an improved cytosine base editor

Plant Physiology, 2021
Abstract Cytosine base editors (CBEs) are the promising tools for precise genome editing in plants. It is important to investigate potential off-target effects of an efficient CBE at the genome and transcriptome levels in a major crop. Based on comparison of five cytidine deaminases and two different promoters for expressing single-guide
Linnell Bentley Randall, Simon Sretenovic, Yuechao Wu, Desuo Yin, Tao Zhang, Joyce Van Eck, Yiping Qi   +6 more
openaire   +2 more sources

Genome sequencing of the extinct Eurasian wild aurochs, Bos primigenius, illuminates the phylogeography and evolution of cattle [PDF]

, 2015
Background Domestication of the now-extinct wild aurochs, Bos primigenius, gave rise to the two major domestic extant cattle taxa, B. taurus and B. indicus.
A Achilli, A Achilli, A Esteve-Codina, A Gotherstrom, A Seguin-Orlando, A Vaysse, A Winter, AE Minoche, AJ Amaral, Alison Murphy, Amanda J. Lohan, Andrew T. Chamberlain, AV Zimin, B Grisart, B Grisart, BP Lewis, Brendan J. Loftus, C Gamba, C Glaser, Ceiridwen J. Edwards, CG Elsik, Charles Spillane, CJ Edwards, CJ Edwards, CJ Edwards, CJ Rubin, CJ Stevens, CM Leu, CS Troy, D Reich, Daniel G. Bradley, David A. Magee, David E. MacHugh, DE MacHugh, DG Bradley, DM Larkin, DP Toews, E Palkopoulou, E Svensson, EJ McTavish, EY Durand, H Jonsson, H Jonsson, H Li, H Zhang, HD Daetwyler, J Clutton-Brock, J Diamond, J Kantanen, J Lenstra, J Schibler, JA Guerra-Assuncao, JD Vigne, JE Decker, JK Pickrell, JK Pritchard, JS Pedersen, K Prufer, KA Moutou, Kévin Rue-Albrecht, L Orlando, L Perez-Pardal, LK Matukumalli, M Gautier, M Hofreiter, M Li, M Meyer, M Raghavan, M Rasmussen, M Schubert, MA DePristo, MA Greagg, MA Groenen, Mark T. Donoghue, Martin Braud, Matthew D. Teasdale, MJ Montague, N Murakami, N Patterson, NA Rosenberg, O Smith, Paul A. McGettigan, R Bollongino, R Chen, RA Gibbs, RE Green, RE Green, RH Meadow, RR Hudson, RT Loftus, S Bonfiglio, S Bonfiglio, S Bonfiglio, S Guindon, S Koks, S Paabo, S Qanbari, S Sawyer, Shuaishuai Tai, Stephen D E Park, Steven Schroeder, Tad S. Sonstegard, TH Lee, W McLaren, Y Benjamini, Yuan Liu   +105 more
core   +1 more source

Computer simulations explain mutation-induced effects on the DNA editing by adenine base editors. [PDF]

, 2020
Adenine base editors, which were developed by engineering a transfer RNA adenosine deaminase enzyme (TadA) into a DNA editing enzyme (TadA*), enable precise modification of A:T to G⋮C base pairs. Here, we use molecular dynamics simulations to uncover the
Komor, Alexis C, Paesani, Francesco, Rallapalli, Kartik L   +2 more
core  

Epigenetics in Friedreich's ataxia: Challenges and opportunities for therapy [PDF]

, 2013
Copyright © 2013 Chiranjeevi Sandi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly ...
Al-Mahdawi, S, Pook, MA, Sandi, C
core   +2 more sources

Engineered Sdd7 cytosine base editors with enhanced specificity

Nature Communications
Cytosine base editors (CBEs) revolutionize genome editing by enabling precise C-to-T conversions without double-strand breaks. Sdd7, a recently developed cytosine deaminase, exhibits high activity across a broad protospacer range but induces unintended off-target effects, including bystander mutations within and upstream of the protospacer and both ...
Hye-Yeon Hwang, Minyoung Lee, Hwalin Yi, Cheong Seok, Kayeong Lim, Yi Rang Na, Jong-Sun Kang, Jae-Hyun Park, Daesik Kim   +8 more
openaire   +2 more sources

Chirality in a quaternionic representation of the genetic code [PDF]

, 2015
A quaternionic representation of the genetic code, previously reported by the authors, is updated in order to incorporate chirality of nucleotide bases and amino acids.
Carlevaro, C. Manuel, Irastorza, Ramiro M., Vericat, Fernando   +2 more
core   +2 more sources

Increasing Cytosine Base Editing Scope and Efficiency With Engineered Cas9-PmCDA1 Fusions and the Modified sgRNA in Rice

Frontiers in Genetics, 2019
Base editors that do not require double-stranded DNA cleavage or homology-directed repair enable higher efficiency and cleaner substitution of targeted single nucleotides in genomic DNA than conventional approaches.
Ying Wu, Wen Xu, Feipeng Wang, Si Zhao, Feng Feng, Jinling Song, Chengwei Zhang, Jinxiao Yang   +7 more
doaj   +1 more source

ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems

BMC Biology, 2020
Background Many favorable traits of crops and livestock and human genetic diseases arise from multiple single nucleotide polymorphisms or multiple point mutations with heterogeneous base substitutions at the same locus.
Jingke Xie, Xingyun Huang, Xia Wang, Shixue Gou, Yanhui Liang, Fangbing Chen, Nan Li, Zhen Ouyang, Quanjun Zhang, Weikai Ge, Qin Jin, Hui Shi, Zhenpeng Zhuang, Xiaozhu Zhao, Meng Lian, Jiaowei Wang, Yinghua Ye, Longquan Quan, Han Wu, Kepin Wang, Liangxue Lai   +20 more
doaj   +1 more source

CABE-RY: A PAM-flexible dual-mutation base editor for reliable modeling of multi-nucleotide variants

Molecular Therapy: Nucleic Acids, 2021
Multi-nucleotide variants (MNVs) represent an important type of genetic variation and have biological and clinical significance. To simulate MNVs, we designed four dual-mutation base editors combining hA3A(Y130F), TadA8e(V106W), and protospacer adjacent ...
Wanyu Tao, Qing Liu, Shisheng Huang, Xin Wang, Shiyuan Qu, Junfan Guo, Danfeng Ou, Guanglei Li, Yu Zhang, Xiangmin Xu, Xingxu Huang   +10 more
doaj   +1 more source