Results 31 to 40 of about 25,918 (263)

Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity [PDF]

Molecular Cell, 2020
Cytosine base editors (CBEs) generate C-to-T nucleotide substitutions in genomic target sites without inducing double-strand breaks. However, CBEs such as BE3 can cause genome-wide off-target changes via sgRNA-independent DNA deamination. By leveraging the orthogonal R-loops generated by SaCas9 nickase to mimic actively transcribed genomic loci that ...
Shuai Jin, Hongyuan Fei, Zixu Zhu, Yingfeng Luo, Jinxing Liu, Shenghan Gao, Feng Zhang, Yu-Hang Chen, Yanpeng Wang, Caixia Gao   +9 more
openaire   +2 more sources

Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos [PDF]

Science, 2019
Erwei Zuo, Yidi Sun, Wu Wei
exaly   +2 more sources

Rewriting Human History and Empowering Indigenous Communities with Genome Editing Tools. [PDF]

, 2020
Appropriate empirical-based evidence and detailed theoretical considerations should be used for evolutionary explanations of phenotypic variation observed in the field of human population genetics (especially Indigenous populations). Investigators within
Fox, Keolu, Komor, Alexis C, Rallapalli, Kartik Lakshmi   +2 more
core   +2 more sources

Beyond seek and destroy: How to generate allelic series using genome editing tools [PDF]

, 2020
Genome editing tools have greatly facilitated the functional analysis of genes of interest by targeted mutagenesis. Many usable genome editing tools, including different site-specific nucleases and editor databases that allow single-nucleotide ...
Bes, Martine, Chaine, Christian, Durandet, Franz, Guiderdoni, Emmanuel, Herbert, Leo, Meunier, Anne Cecile, Michel, Rémy, Portefaix, Murielle, Périn, Christophe, This, Patrice, Vernet, Aurore   +10 more
core   +3 more sources

Developing high-efficiency base editors by combining optimized synergistic core components with new types of nuclear localization signal peptide

Crop Journal, 2020
The clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein (Cas) system has been widely used for genome editing. In this system, the cytosine base editor (CBE) and adenine base editor (ABE) allow generating precise ...
Feipeng Wang, Chengwei Zhang, Wen Xu, Shuang Yuan, Jinling Song, Lu Li, Jiuran Zhao, Jinxiao Yang   +7 more
doaj   +1 more source

Improved cytosine base editors generated from TadA variants

Nature Biotechnology, 2023
AbstractCytosine base editors (CBEs) enable programmable genomic C·G-to-T·A transition mutations and typically comprise a modified CRISPR–Cas enzyme, a naturally occurring cytidine deaminase, and an inhibitor of uracil repair. Previous studies have shown that CBEs utilizing naturally occurring cytidine deaminases may cause unguided, genome-wide ...
Lam, Dieter K., Feliciano, Patricia R., Arif, Amena, Bohnuud, Tanggis, Fernandez, Thomas P., Gehrke, Jason M., Grayson, Phil, Lee, Kin D., Ortega, Manuel A., Sawyer, Courtney, Schwaegerle, Noah D., Peraro, Leila, Young, Lauren, Lee, Seung-Joo, Ciaramella, Giuseppe, Gaudelli, Nicole M.   +15 more
openaire   +3 more sources

Examination of the Cell Cycle Dependence of Cytosine and Adenine Base Editors

Frontiers in Genome Editing, 2022
Base editors (BEs) are genome editing agents that install point mutations with high efficiency and specificity. Due to their reliance on uracil and inosine DNA damage intermediates (rather than double-strand DNA breaks, or DSBs), it has been hypothesized that BEs rely on more ubiquitous DNA repair pathways than DSB-reliant genome editing methods, which
Cameron A. Burnett, Ashley T. Wong, Carlos A. Vasquez, Colleen A. McHugh, Gene W. Yeo, Gene W. Yeo, Alexis C. Komor   +6 more
openaire   +5 more sources

Development of a Simple and Quick Method to Assess Base Editing in Human Cells

Molecular Therapy: Nucleic Acids, 2020
Base editing is a form of genome editing that can directly convert a single base (C or A) to another base (T or G), which is of great potential in biomedical applications.
Xiujuan Lv, Kairui Qiu, Tianxiang Tu, Xiaoxue He, Yuchen Peng, Jinbin Ye, Junhao Fu, Ruzhi Deng, Yuqin Wang, Jinyu Wu, Changbao Liu, Junzhao Zhao, Feng Gu   +12 more
doaj   +1 more source

Targeting fidelity of adenine and cytosine base editors in mouse embryos [PDF]

Nature Communications, 2018
AbstractBase editing directly converts a target base pair into a different base pair in the genome of living cells without introducing double-stranded DNA breaks. While cytosine base editors (CBE) and adenine base editors (ABE) are used to install and correct point mutations in a wide range of organisms, the extent and distribution of off-target edits ...
Hye Kyung Lee, Michaela Willi, Shannon M. Miller, Sojung Kim, Chengyu Liu, David R. Liu, Lothar Hennighausen   +6 more
openaire   +3 more sources

CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants

Synthetic and Systems Biotechnology, 2020
CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) has been extensively exploited as a genetic tool for genome editing.
Abdullah, Zhengzheng Jiang, Xulin Hong, Shun Zhang, Ruilian Yao, Yi Xiao   +5 more
doaj   +1 more source