Results 31 to 40 of about 15,403 (255)

A cytosine base editor toolkit with varying activity windows and target scopes for versatile gene manipulation in plants [PDF]

Nucleic Acids Research, 2022
Xiangyu Xiong, Zhenxiang Li, Chenlong Li   +2 more
exaly   +2 more sources

Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion

Genome Biology, 2018
Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9, enabling A•T to G•C conversion at ...
Chao Li, Yuan Zong, Yanpeng Wang, Shuai Jin, Dingbo Zhang, Qianna Song, Rui Zhang, Caixia Gao   +7 more
doaj   +2 more sources

High performance TadA-8e derived cytosine and dual base editors with undetectable off-target effects in plants

Nature Communications
Cytosine base editors (CBEs) and adenine base editors (ABEs) enable precise C-to-T and A-to-G edits. Recently, ABE8e, derived from TadA-8e, enhances A-to-G edits in mammalian cells and plants.
Tingting Fan, Yanhao Cheng, Yuechao Wu, Shishi Liu, Xu Tang, Yao He, Shanyue Liao, Xuelian Zheng, Tao Zhang, Yiping Qi, Yong Zhang   +10 more
doaj   +2 more sources

Highly efficient CRISPR-Cas9 base editing in Bifidobacterium with bypass of restriction modification systems

Applied and Environmental Microbiology
Intestinal microbiota members of the Bifidobacterium genus are increasingly explored as probiotics and therapeutics. However, the paucity of genetic tools and the widespread restriction modification (RM) systems in Bifidobacterium limit our ability to ...
Hung-Chun Lin, Wan-Chi Hsiao, Ya-Chen Hsu, Meng-Chieh Lin, Cheng-Chih Hsu, Mingzi M. Zhang   +5 more
doaj   +2 more sources

Cytosine base editor 4 but not adenine base editor generates off-target mutations in mouse embryos. [PDF]

Commun Biol, 2020
AbstractDeaminase base editing has emerged as a tool to install or correct point mutations in the genomes of living cells in a wide range of organisms. However, the genome-wide off-target effects introduced by base editors in the mammalian genome have been examined in only one study.
Lee HK, Smith HE, Liu C, Willi M, Hennighausen L.   +4 more
europepmc   +4 more sources

Characterizing and Reducing Spurious DNA Edits by CRISPR Cytosine Base Editors

, 2020
Cytosine Base Editors (CBEs) enable precise cytosine-to-thymine genetic mutations via APOBEC-mediated deamination of CRISPR-targeted cytosines. However, due to the natural RNA- and DNA-editing capacity of the APOBEC domains they harbor, CBEs possess the ...
Angstman, James
core   +2 more sources

Development of an Efficient C-to-T Base-Editing System and Its Application to Cellulase Transcription Factor Precise Engineering in Thermophilic Fungus Myceliophthora thermophila

Microbiology Spectrum, 2022
Myceliophthora thermophila is a thermophilic fungus with great potential in biorefineries and biotechnology. The base editor is an upgraded version of the clustered regularly interspaced short palindromic repeats (CRISPR)-dependent genome-editing tool ...
Chenyang Zhang, Nan Li, Lang Rao, Jingen Li, Qian Liu, Chaoguang Tian   +5 more
doaj   +1 more source

Base Editors for Citrus Gene Editing

Frontiers in Genome Editing, 2022
Base editors, such as adenine base editors (ABE) and cytosine base editors (CBE), provide alternatives for precise genome editing without generating double-strand breaks (DSBs), thus avoiding the risk of genome instability and unpredictable outcomes ...
Xiaoen Huang, Yuanchun Wang, Nian Wang
doaj   +1 more source

Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos [PDF]

Science, 2019
Erwei Zuo, Yidi Sun, Wu Wei
exaly   +2 more sources

Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity [PDF]

Molecular Cell, 2020
Cytosine base editors (CBEs) generate C-to-T nucleotide substitutions in genomic target sites without inducing double-strand breaks. However, CBEs such as BE3 can cause genome-wide off-target changes via sgRNA-independent DNA deamination. By leveraging the orthogonal R-loops generated by SaCas9 nickase to mimic actively transcribed genomic loci that ...
Shuai Jin, Hongyuan Fei, Zixu Zhu, Yingfeng Luo, Jinxing Liu, Shenghan Gao, Feng Zhang, Yu-Hang Chen, Yanpeng Wang, Caixia Gao   +9 more
openaire   +2 more sources