Results 31 to 40 of about 15,248 (235)

Development of a Simple and Quick Method to Assess Base Editing in Human Cells

Molecular Therapy: Nucleic Acids, 2020
Base editing is a form of genome editing that can directly convert a single base (C or A) to another base (T or G), which is of great potential in biomedical applications.
Xiujuan Lv, Kairui Qiu, Tianxiang Tu, Xiaoxue He, Yuchen Peng, Jinbin Ye, Junhao Fu, Ruzhi Deng, Yuqin Wang, Jinyu Wu, Changbao Liu, Junzhao Zhao, Feng Gu   +12 more
doaj   +1 more source

CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants

Synthetic and Systems Biotechnology, 2020
CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) has been extensively exploited as a genetic tool for genome editing.
Abdullah, Zhengzheng Jiang, Xulin Hong, Shun Zhang, Ruilian Yao, Yi Xiao   +5 more
doaj   +1 more source

Improved cytosine base editors generated from TadA variants

Nature Biotechnology, 2023
AbstractCytosine base editors (CBEs) enable programmable genomic C·G-to-T·A transition mutations and typically comprise a modified CRISPR–Cas enzyme, a naturally occurring cytidine deaminase, and an inhibitor of uracil repair. Previous studies have shown that CBEs utilizing naturally occurring cytidine deaminases may cause unguided, genome-wide ...
Lam, Dieter K., Feliciano, Patricia R., Arif, Amena, Bohnuud, Tanggis, Fernandez, Thomas P., Gehrke, Jason M., Grayson, Phil, Lee, Kin D., Ortega, Manuel A., Sawyer, Courtney, Schwaegerle, Noah D., Peraro, Leila, Young, Lauren, Lee, Seung-Joo, Ciaramella, Giuseppe, Gaudelli, Nicole M.   +15 more
openaire   +3 more sources

In vivo mitochondrial base editing via adeno-associated viral delivery to mouse post-mitotic tissue

Nature Communications, 2022
Mutations in mitochondrial DNA can lead to clinically heterogeneous disease. Here the authors demonstrate in vivo base editing of mouse mitochondrial DNA in a post-mitotic tissue by AAV delivery of DddA-derived cytosine base editor (DdCBE).
Pedro Silva-Pinheiro, Pavel A. Nash, Lindsey Van Haute, Christian D. Mutti, Keira Turner, Michal Minczuk   +5 more
doaj   +1 more source

Targeting fidelity of adenine and cytosine base editors in mouse embryos [PDF]

Nature Communications, 2018
AbstractBase editing directly converts a target base pair into a different base pair in the genome of living cells without introducing double-stranded DNA breaks. While cytosine base editors (CBE) and adenine base editors (ABE) are used to install and correct point mutations in a wide range of organisms, the extent and distribution of off-target edits ...
Hye Kyung Lee, Michaela Willi, Shannon M. Miller, Sojung Kim, Chengyu Liu, David R. Liu, Lothar Hennighausen   +6 more
openaire   +3 more sources

Examination of the Cell Cycle Dependence of Cytosine and Adenine Base Editors

Frontiers in Genome Editing, 2022
Base editors (BEs) are genome editing agents that install point mutations with high efficiency and specificity. Due to their reliance on uracil and inosine DNA damage intermediates (rather than double-strand DNA breaks, or DSBs), it has been hypothesized that BEs rely on more ubiquitous DNA repair pathways than DSB-reliant genome editing methods, which
Cameron A. Burnett, Ashley T. Wong, Carlos A. Vasquez, Colleen A. McHugh, Gene W. Yeo, Gene W. Yeo, Alexis C. Komor   +6 more
openaire   +5 more sources

C-to-G Base Editing Enhances Oleic Acid Production by Generating Novel Alleles of FATTY ACID DESATURASE 2 in Plants

Frontiers in Plant Science, 2021
The demand for vegetable oil, which is mainly used for dietary purposes and cooking, is steadily increasing worldwide. It is often desirable to reduce unsaturation levels of fatty acids in order to increase storage stability and reduce trans-fat ...
Mid-Eum Park, Jae-Young Yun, Hyun Uk Kim, Hyun Uk Kim   +3 more
doaj   +1 more source

Optimization of the base editor BE4max in chicken somatic cells

Poultry Science, 2022
: Advanced animal reproductive and breeding biotechnology has made it possible to alter traits or create new genetic resources by the direct knock-in or knock-out of target genes.
Tianpeng Xu, Jing Zhong, Zhenwen Huang, Lintian Yu, Jitan Zheng, Long Xie, Lingling Sun, Xingting Liu, Yangqing Lu   +8 more
doaj   +1 more source

Highly efficient CRISPR-SaKKH tools for plant multiplex cytosine base editing

Crop Journal, 2020
Base editing, as an expanded clustered regularly interspaced short palindromic repeats (CRISPR)-Cas genome editing strategy, permits precise and irreversible nucleotide conversion. SaKKH, an efficient variant of a Cas9 ortholog from Staphylococcus aureus
Chengwei Zhang, Feipeng Wang, Si Zhao, Guiting Kang, Jinling Song, Lu Li, Jinxiao Yang   +6 more
doaj   +1 more source

Sequence-specific prediction of the efficiencies of adenine and cytosine base editors

Nature Biotechnology, 2020
Base editors, including adenine base editors (ABEs)1 and cytosine base editors (CBEs)2,3, are widely used to induce point mutations. However, determining whether a specific nucleotide in its genomic context can be edited requires time-consuming experiments.
Myungjae Song, Hui Kwon Kim, Sungtae Lee, Younggwang Kim, Sang-Yeon Seo, Jinman Park, Jae Woo Choi, Hyewon Jang, Jeong Hong Shin, Seonwoo Min, Zhejiu Quan, Ji Hun Kim, Hoon Chul Kang, Sungroh Yoon, Hyongbum Henry Kim   +14 more
openaire   +3 more sources