Small-molecule compounds boost genome-editing efficiency of cytosine base editor [PDF]
Nucleic Acids Research, 2021 Abstract Cytosine base editor (CBE) enables targeted C-to-T conversions at single base-pair resolution and thus has potential therapeutic applications in humans. However, the low efficiency of the system limits practical use of this approach.
Qing Li, Zongming Song, Haoyi Wang
exaly +5 more sources
Efficient CRISPR-Cas9 based cytosine base editors for phytopathogenic bacteria
Communications Biology, 2023 A versatile cytosine base editor system is developed to achieve highly efficient C to T conversion in the genome of a series of phytopathogenic bacteria, including Xanthomonas, Pseudomonas, Erwinia, and Agrobacterium.
Chenhao Li +9 more
doaj +4 more sources
Engineering soybean with high levels of herbicide resistance with a Cas12‐SF01‐based cytosine base editor [PDF]
Plant Biotechnology JournalQingfeng Niu +2 more
exaly +4 more sources
Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors
Communications Biology, 2022 Cas12a-mediated cytosine (CBE) and adenine base editor (ABE) systems with elevated efficiencies and expanded target scopes are developed, by combining highly active deaminases with Cas12a variants, enCas12a, RR and RVR, recognizing non-TTTV PAMs.
Fangbing Chen +23 more
doaj +3 more sources
Development of plant cytosine base editors with the Cas12a system
Crop Journal, 2023 Base editors of the Cas9 system have been widely used for precise nucleotide substitution in crops. In this study, Cas12a was applied to construct plant cytosine base editors (CBEs).
Huanhuan Wang +15 more
doaj +2 more sources
Cytosine base editors optimized for genome editing in potato protoplasts
Frontiers in Genome Editing, 2023 In this study, we generated and compared three cytidine base editors (CBEs) tailor-made for potato (Solanum tuberosum), which conferred up to 43% C-to-T conversion of all alleles in the protoplast pool.
Ida Westberg +3 more
doaj +5 more sources
Cytosine base editors (CBEs) for inducing targeted DNA base editing in Nicotiana benthamiana
BMC Plant Biology, 2023 Background The base editors can introduce point mutations accurately without causing double-stranded DNA breaks or requiring donor DNA templates. Previously, cytosine base editors (CBEs) containing different deaminases are reported for precise and ...
Juan Luo +6 more
doaj +4 more sources
Microbiology Spectrum, 2022 Genome editing technology is a powerful tool for programming microbial cell factories. However, rat APOBEC1-derived cytosine base editor (CBE) that converts C•G to T•A at target genes induced DNA off-targets, regardless of single-guide RNA (sgRNA ...
Yu Been Heo +4 more
doaj +3 more sources
Research progress of base editing and prime editing tools based on the CRISPR/Cas system [PDF]
Molecular Therapy: Nucleic AcidsThe base editor (BE) and prime editing guide RNA (pegRNA)-based prime editor (PE) technologies relying on the CRISPR/Cas system are very efficient gene editors that have been developed in recent years.
Herui Gao +4 more
doaj +2 more sources
Eliminating predictable DNA off-target effects of cytosine base editor by using dual guiders including sgRNA and TALE [PDF]
Molecular Therapy, 2022 Yang Yang +2 more
exaly +2 more sources