Adenine base editor engineering reduces editing of bystander cytosines [PDF]
Nature Biotechnology, 2021 Adenine base editors (ABEs) catalyze specific A-to-G conversions at genomic sites of interest. However, ABEs also induce cytosine deamination at the target site.
Hong, Sung-Ah +8 more
core +4 more sources
Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells [PDF]
Genome BiologyBase editors are precise editing tools that employ deaminases to modify target DNA bases. The DYW-family of cytosine deaminases is structurally and phylogenetically distinct and might be harnessed for genome editing tools.
Dingbo Zhang +5 more
doaj +2 more sources
Engineering TadA ortholog-derived cytosine base editor without motif preference and adenosine activity limitation [PDF]
Nature CommunicationsThe engineered TadA variants used in cytosine base editors (CBEs) present distinctive advantages, including a smaller size and fewer off-target effects compared to cytosine base editors that rely on natural deaminases.
Guoling Li +17 more
doaj +2 more sources
Cytosine Base Editor-Mediated Multiplex Genome Editing to Accelerate Discovery of Novel Antibiotics in Bacillus subtilis and Paenibacillus polymyxa. [PDF]
Front Microbiol, 2021 Genome-based identification of new antibiotics is emerging as an alternative to traditional methods. However, uncovering hidden antibiotics under the background of known antibiotics remains a challenge.
Kim MS, Kim HR, Jeong DE, Choi SK.
europepmc +2 more sources
Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors
Communications Biology, 2022 Cas12a-mediated cytosine (CBE) and adenine base editor (ABE) systems with elevated efficiencies and expanded target scopes are developed, by combining highly active deaminases with Cas12a variants, enCas12a, RR and RVR, recognizing non-TTTV PAMs.
Fangbing Chen +23 more
doaj +3 more sources
Development of plant cytosine base editors with the Cas12a system
Crop Journal, 2023 Base editors of the Cas9 system have been widely used for precise nucleotide substitution in crops. In this study, Cas12a was applied to construct plant cytosine base editors (CBEs).
Huanhuan Wang +15 more
doaj +2 more sources
Cytosine Base Editor (hA3A-BE3-NG)-Mediated Multiple Gene Editing for Pyramid Breeding in Pigs [PDF]
Frontiers in Genetics, 2020 Pig is an important agricultural economic animal, providing large amount of meat products. With the development of functional genomics and bioinformatics, lots of genes and functional single nucleotide polymorphisms (SNPs) related to disease resistance ...
Yu Wang +16 more
doaj +2 more sources
Adenine base editors catalyze cytosine conversions in human cells
Nature Biotechnology, 2019 Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window ...
Sangsu Bae +9 more
core +3 more sources
Nature CommunicationsCytosine base editing is a powerful tool for making precise single nucleotide changes in cells and model organisms like zebrafish, which are valuable for studying human diseases.
Yu Zhang +13 more
doaj +2 more sources
Highly efficient CRISPR-SaKKH tools for plant multiplex cytosine base editing
Crop Journal, 2020 Base editing, as an expanded clustered regularly interspaced short palindromic repeats (CRISPR)-Cas genome editing strategy, permits precise and irreversible nucleotide conversion. SaKKH, an efficient variant of a Cas9 ortholog from Staphylococcus aureus
Chengwei Zhang +6 more
doaj +3 more sources