Results 11 to 20 of about 15,248 (235)

Cytosine base editor-DNA binding domain fusions for editing window modulation in the RNP format [PDF]

BMC Biotechnology
Summary Base editing technologies allow for the precise and efficient installation of defined nucleotide substitutions into a target genome without the introduction of double strand breaks or DNA templates.
Erin Brettmann, Fuqiang Chen, Stephen Beishir, Graeme Garvey   +3 more
doaj   +2 more sources

Gene editing and scalable functional genomic screening in Leishmania species using the CRISPR/Cas9 cytosine base editor toolbox LeishBASEedit [PDF]

eLife, 2023
CRISPR/Cas9 gene editing has revolutionised loss-of-function experiments in Leishmania, the causative agent of leishmaniasis. As Leishmania lack a functional non-homologous DNA end joining pathway however, obtaining null mutants typically requires ...
Markus Engstler, Tom Beneke
doaj   +2 more sources

Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors

Communications Biology, 2022
Cas12a-mediated cytosine (CBE) and adenine base editor (ABE) systems with elevated efficiencies and expanded target scopes are developed, by combining highly active deaminases with Cas12a variants, enCas12a, RR and RVR, recognizing non-TTTV PAMs.
Fangbing Chen, Meng Lian, Bingxiu Ma, Shixue Gou, Xian Luo, Kaiming Yang, Hui Shi, Jingke Xie, Weika Ge, Zhen Ouyang, Chengdan Lai, Nan Li, Quanjun Zhang, Qin Jin, Yanhui Liang, Tao Chen, Jiaowei Wang, Xiaozhu Zhao, Lei Li, Manya Yu, Yinghua Ye, Kepin Wang, Han Wu, Liangxue Lai   +23 more
doaj   +3 more sources

Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity [PDF]

Molecular Cell, 2020
Cytosine base editors (CBEs) generate C-to-T nucleotide substitutions in genomic target sites without inducing double-strand breaks. However, CBEs such as BE3 can cause genome-wide off-target changes via sgRNA-independent DNA deamination. By leveraging the orthogonal R-loops generated by SaCas9 nickase to mimic actively transcribed genomic loci that ...
, Yong Chen
exaly   +3 more sources

Development of plant cytosine base editors with the Cas12a system

Crop Journal, 2023
Base editors of the Cas9 system have been widely used for precise nucleotide substitution in crops. In this study, Cas12a was applied to construct plant cytosine base editors (CBEs).
Huanhuan Wang, Jing Liang, Like Chen, Bufang Deng, Dongfang Gu, Xiaoshuang Liu, Shan Jin, Rongfang Xu, Ruiying Qin, Yitong Zhu, Liangxia Zhao, Dourong Kou, Yanjun Chen, Yingli Jiang, Juan Li, Pengcheng Wei   +15 more
doaj   +2 more sources

Cytosine Base Editor (hA3A-BE3-NG)-Mediated Multiple Gene Editing for Pyramid Breeding in Pigs [PDF]

Frontiers in Genetics, 2020
Pig is an important agricultural economic animal, providing large amount of meat products. With the development of functional genomics and bioinformatics, lots of genes and functional single nucleotide polymorphisms (SNPs) related to disease resistance ...
Yu Wang, Yu Wang, Dengfeng Bi, Dengfeng Bi, Guosong Qin, Ruigao Song, Ruigao Song, Jing Yao, Chunwei Cao, Chunwei Cao, Qiantao Zheng, Naipeng Hou, Naipeng Hou, Yanfang Wang, Yanfang Wang, Jianguo Zhao, Jianguo Zhao   +16 more
doaj   +2 more sources

A universal and wide-range cytosine base editor via domain-inlaid and fidelity-optimized CRISPR-FrCas9 [PDF]

Nature Communications
CRISPR-based base editor (BE) offer diverse editing options for genetic engineering of microorganisms, but its application is limited by protospacer adjacent motif (PAM) sequences, context preference, editing window, and off-target effects.
Lan Hu, Jing Han, Hao-Da Wang, Zhou-Hua Cheng, Chang-Ce Lv, Dong-Feng Liu, Han-Qing Yu   +6 more
doaj   +2 more sources

Cytosine base editors (CBEs) for inducing targeted DNA base editing in Nicotiana benthamiana

BMC Plant Biology, 2023
Background The base editors can introduce point mutations accurately without causing double-stranded DNA breaks or requiring donor DNA templates. Previously, cytosine base editors (CBEs) containing different deaminases are reported for precise and ...
Juan Luo, Muhammad Abid, Jing Tu, Xinxia Cai, Yi Zhang, Puxin Gao, Hongwen Huang   +6 more
doaj   +4 more sources

Engineered cytosine base editor enabling broad-scope and high-fidelity gene editing in Streptomyces [PDF]

Nature Communications
Base editing (BE) faces protospacer adjacent motif (PAM) constraints and off-target effects in both eukaryotes and prokaryotes. For Streptomyces, renowned as one of the most prolific bacterial producers of antibiotics, the challenges are more pronounced ...
Jian Wang, Ke Wang, Zhe Deng, Zhiyu Zhong, Guo Sun, Qing Mei, Fuling Zhou, Zixin Deng, Yuhui Sun   +8 more
doaj   +2 more sources

Increasing Cytosine Base Editing Scope and Efficiency With Engineered Cas9-PmCDA1 Fusions and the Modified sgRNA in Rice

Frontiers in Genetics, 2019
Base editors that do not require double-stranded DNA cleavage or homology-directed repair enable higher efficiency and cleaner substitution of targeted single nucleotides in genomic DNA than conventional approaches.
Ying Wu, Wen Xu, Feipeng Wang, Si Zhao, Feng Feng, Jinling Song, Chengwei Zhang, Jinxiao Yang   +7 more
doaj   +3 more sources